Spheroids Figures, text etc (Column 1) Synthesis of Calcium - - PowerPoint PPT Presentation

L.I. Fatkhutdinova1, H. Bahrom2, A.A. Goncharenko3, O. Peltek1, A. Muslimov4, A. Manchev2,

• I. Shishkin1, R.E. Noskov2, A.S. Timin3, P. Ginzburg2 and M.V. Zyuzin1

Figures, text etc (Column 1)

Spheroids

1 ITMO University,

Saint Petersburg, Russian Federation

2 Tel-Aviv University,

Tel-Aviv, Israel

3 Peter the Great St. Petersburg

Polytechnic University, Saint Petersburg, Russian Federation

4 St. Petersburg Academic University,

Saint Petersburg, Russian Federation

Synthesis of Calcium Carbonate Particles with Different Geometries

Introduction Particle formation Thermal behaviour Surface properties In vitro studies Conclusions

L.I. Fatkhutdinova1, H. Bahrom2, A.A. Goncharenko3, O. Peltek1, A. Muslimov4, A. Manchev2,

• I. Shishkin1, R.E. Noskov2, A.S. Timin3, P. Ginzburg2 and M.V. Zyuzin1

Synthesis of Calcium Carbonate Particles with Different Geometries

1 ITMO University,

Saint Petersburg, Russian Federation

2 Tel-Aviv University,

Tel-Aviv, Israel

3 Peter the Great St. Petersburg

Polytechnic University, Saint Petersburg, Russian Federation

4 St. Petersburg Academic University,

Saint Petersburg, Russian Federation

Relevance

controllable synthesis of different shaped particles

nanoparticles:

by changing the synthetic conditions control size, shape and porosity controllable physicochemical properties

loading capacity stability sustained drug release delivery platform for biologically active compounds

Why Calcium Carbonate? low cost

biocompatibility biodegradability

safety pH-sensitivity

biocompatible containers for drug delivery into cells

porosity Vaterite [1]

References

[1] Trofimov, A. D. et. al. Pharmaceutics, 10(4), 167

Introduction Particle formation Thermal behaviour Surface properties In vitro studies Conclusions

Introduction Particle formation Thermal behaviour Surface properties In vitro studies Conclusions

Influence of salt concentrations

L.I. Fatkhutdinova, H. Bahrom, A.A. Goncharenko, O. Peltek, A. Muslimov, A. Manchev,

• I. Shishkin, R.E. Noskov, A.S. Timin, P. Ginzburg and M.V. Zyuzin

Reaction A-I Reaction A-II Reaction A-III

SEM images of products of reactions A-I, A-II, A-III (Molar salt ratio = 1:1) in 5 min

c(CaCl2) = 5 ∙ 10-4 M c(Na2CO3) = 5 ∙ 10-4 M c(CaCl2) = 5 ∙ 10-3 M c(Na2CO3) = 5 ∙ 10-3 M c(CaCl2) = 5 ∙ 10-2 M c(Na2CO3) = 5 ∙ 10-2 M Molar salt ratio = 1:1 Molar salt ratio = 1:1 Molar salt ratio = 1:1

Synthesis of Calcium Carbonate Particles with Different Geometries

Introduction Particle formation Thermal behaviour Surface properties In vitro studies Conclusions

Dependence on the reaction time & ion excess concentration

L.I. Fatkhutdinova, H. Bahrom, A.A. Goncharenko, O. Peltek, A. Muslimov, A. Manchev,

• I. Shishkin, R.E. Noskov, A.S. Timin, P. Ginzburg and M.V. Zyuzin

Synthesis of Calcium Carbonate Particles with Different Geometries

B-I B-II B-III 30 min 60 min 24 h CaCl2 : Na2CO3 5:1 1:1 1:5 SEM images of reactions B-I, B-II, B-III in 30 min, 60 min, 24 h Reaction

Introduction Particle formation Thermal behaviour Surface properties In vitro studies Conclusions

Influence of HCO3

• ions

L.I. Fatkhutdinova, H. Bahrom, A.A. Goncharenko, O. Peltek, A. Muslimov, A. Manchev,

• I. Shishkin, R.E. Noskov, A.S. Timin, P. Ginzburgand M.V. Zyuzin

Synthesis of Calcium Carbonate Particles with Different Geometries

C-I 5:1 C-II 1:1 C-III 1:5 C-IV 1:10 C-V 1:15 CaCl2 : NaHCO3

Reaction

Introduction Particle formation Thermal behaviour Surface properties In vitro studies Conclusions

Influence of organic additive

L.I. Fatkhutdinova, H. Bahrom, A.A. Goncharenko, O. Peltek, A. Muslimov, A. Manchev,

• I. Shishkin, R.E. Noskov, A.S. Timin, P. Ginzburg and M.V. Zyuzin

Synthesis of Calcium Carbonate Particles with Different Geometries

SEM images of products of reactions D-I, D-II, D-III, in 24 h

Scheme of toroid formation by introducing PSS as organic additive XRD analysis of CaCO3 samples obtained by reactions D-I, D-II, D-III Molar ratio CaCl2 : Na2CO3

D-I D-II D-III 5:1 5:1 5:1

Reaction с(PSS), mg/mL 0.5 1

poly(styrene sulfonate) sodium – PSS

2

Introduction Particle formation Thermal behaviour Surface properties In vitro studies Conclusions

Conclusion

L.I. Fatkhutdinova, H. Bahrom, A.A. Goncharenko, O. Peltek, A. Muslimov, A. Manchev,

• I. Shishkin, R.E. Noskov, A.S. Timin, P. Ginzburg and M.V. Zyuzin

Synthesis of Calcium Carbonate Particles with Different Geometries

Introduction Particle formation Surface properties In vitro studies Conclusions L.I. Fatkhutdinova, H. Bahrom, A.A. Goncharenko, O. Peltek, A. Muslimov, A. Manchev,

• I. Shishkin, R.E. Noskov, A.S. Timin, P. Ginzburg and M.V. Zyuzin

Synthesis of Calcium Carbonate Particles with Different Geometries

Thermal behaviour

Differential scanning calorimetry (DSC) Thermogravimetric analysis (TGA)

Introduction Particle formation Thermal behaviour Surface properties In vitro studies Conclusions

Surface properties of differently shaped CaCO3 particles

L.I. Fatkhutdinova, H. Bahrom, A.A. Goncharenko, O. Peltek, A. Muslimov, A. Manchev,

• I. Shishkin, R.E. Noskov, A.S. Timin, P. Ginzburg and M.V. Zyuzin

Synthesis of Calcium Carbonate Particles with Different Geometries

Percentage of released TRITC from CaCO3 particles shaken in water with time (0-24 h) Adsorption isotherm curves for TRITC adsorption of CaCO3 particles. Adsorption capacity of spherical (red), toroidal (blue) and ellipsoidal (green) CaCO3 particles incubated with TRITC at concentration Ce = 0.5 mg/mL.

b – the Langmuir isotherm constant Qmax – theoretical monolayer saturation capacity of the TRITC C – equilibrium concentration of TRITC

Introduction Particle formation Thermal behaviour Surface properties In vitro studies Conclusions

Surface properties of differently shaped CaCO3 particles

L.I. Fatkhutdinova, H. Bahrom, A.A. Goncharenko, O. Peltek, A. Muslimov, A. Manchev,

• I. Shishkin, R.E. Noskov, A.S. Timin, P. Ginzburg and M.V. Zyuzin

Synthesis of Calcium Carbonate Particles with Different Geometries

Pore volume, cm3/g Pore diameter, nm ζ-potential, mV

Spheroids Toroids Ellipsoids

0.127

• 2

0.084 3.57

• 12

0.172

• 0.8

Specific surface area, m2/g 40.6 75.5 43.1 12.7 15.7

Introduction Particle formation Thermal behaviour Surface properties In vitro studies Conclusions L.I. Fatkhutdinova, H. Bahrom, A.A. Goncharenko, O. Peltek, A. Muslimov, A. Manchev,

• I. Shishkin, R.E. Noskov, A.S. Timin, P. Ginzburg and M.V. Zyuzin

Synthesis of Calcium Carbonate Particles with Different Geometries

Cytotoxicity Uptake efficiency

Z-stack CLSM image confirming the internalization of particles labeled with TRITC with corresponding 3D reconstruction image of cells with internalized particles.

2 4 6 8 10 12 14 4 8 12 16 20 24 28

f(x) x

Spheres Toroids Ellipsoids

Frequency f(x)

• f

cells which have internalized x particles per cell after 24 h of incubation cell-to-particle ratio = 1:10

C6 glioma cells viability after incubation with different shaped CaCO3 particles.

Toroids

Cntr Cntr Cntr

Introduction Particle formation Thermal behaviour Surface properties In vitro studies Conclusions

Surface properties of differently shaped CaCO3 particles

L.I. Fatkhutdinova, H. Bahrom, A.A. Goncharenko, O. Peltek, A. Muslimov, A. Manchev,

• I. Shishkin, R.E. Noskov, A.S. Timin, P. Ginzburg and M.V. Zyuzin

Synthesis of Calcium Carbonate Particles with Different Geometries

uptake efficiency study on a sample of C6 glioma cells: cell uptake depended on the shape of the CaCO3 particles ellipsoids had the highest internalizing rate cytotoxicity study: the different shaped CaCO3 particles used did not show any cytotoxic effects the crystallinity of the resultant CaCO3 particles depends on reaction conditions variations in reaction conditions allowed the formation of different shaped CaCO3 particles (spheroids, ellipsoids and toroids) adsorption efficiency: toroids are characterized by the highest adsorption capacity non-toxicity biocompatibility The outstanding surface properties of these CaCO3 particles together with their solubility enables them to be used as drug delivery carriers