Resources and Opportunities Johns Hopkins Malaria Research Institute - - PowerPoint PPT Presentation

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Resources and Opportunities Johns Hopkins Malaria Research Institute - - PowerPoint PPT Presentation

Oct 13, 2022 363 likes •865 views

Special Seminar 21 September 2018 Resources and Opportunities Johns Hopkins Malaria Research Institute Department of Molecular Microbiology and Immunology Johns Hopkins Bloomberg School of Public Health Connection with MMI Since 2001

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Resources and Opportunities

Special Seminar

21 September 2018

Johns Hopkins Malaria Research Institute

Department of Molecular Microbiology and Immunology Johns Hopkins Bloomberg School of Public Health

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Since 2001 Bloomberg Philanthropies has given $125 million to the Bloomberg School to fund a state-of-the-art research facility to mount a broad program

  • f

basic-science research to treat and control malaria, develop a vaccine and find new drug targets to prevent and cure this deadly disease.

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Diane Griffin, MD, PhD Founding Director, JHMRI Former Chair, MMI Dept

The Institute resides in the Department of Molecular Microbiology and Immunology (MMI).

Arturo Casadevall, MD, PhD Current Chair, MMI Dept

Connection with MMI

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Leadership

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Peter Agre, MD

Bloomberg Distinguished Professor & JHMRI Director

George Dimopoulos, PhD, MBA

Professor, JHMRI Deputy Director, Parasite Core Director

Bill Moss, MD, MPH

Professor, JHMRI Deputy Director, & ICEMR Program Director

Photini Sinnis, MD

Professor & JHMRI Deputy Director

Sean Prigge, PhD

Professor, JHMRI Deputy Director, & Co-Director Biophysics Core

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School of Public Health – Molecular Microbiology & Immunology – Epidemiology School of Medicine – Cell Biology – Neuroscience – Pharmacology

  • Insectary
  • Parasite Core
  • Biophysics Core
  • Spatial Science Core
  • MMI Genetic Analysis &

Sequencing Facility

  • MMI Imaging & Microscopy
  • MMI Flow Cytometry/Cell

Sorting Core

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Macha, Zambia

21 Faculty/ Labs Core Facilities Field Site

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Field station in rural Zambia Molecular laboratory Insectary Malaria “sphere” Flight cage

Macha Research Trust Southern Province, Zambia

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  • Dr. Phil Thuma, MD

Senior Scientific advisor

  • Dr. Jennifer Stevenson, PhD

Lead Entomologist

JHBSPH Staff Based in Macha

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Biophysics Core

Facility Co-Directors

Scott Bailey, PhD Email: scott.bailey@jhu.edu Sean Prigge, PhD Email: sprigge2@jhu.edu

Biophysics Core Johns Hopkins Bloomberg School of Public Health 615 N. Wolfe Street, Room W8620 Baltimore, Maryland 21205 Telephone: 443-287-48227 fax: 410-955-0105

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Biophysics Core

Isothermal Titration Calorimetry: This technique measures the heat of binding between two molecules, providing a direct measurement of binding affinity. Equipment: MicroCal VP-ITC Location: W8620 The Biophysics Core Facility provides equipment for JHMRI investigators who want to characterize macromolecules or macromolecular complexes using biophysical techniques

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Biophysics Core

Dynamic Light Scattering. This technique provides information about the size, shape and oligomerization state of biomolecules. Equipment: Proterion DynaPro with Peltier temperature control Location: W8620

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Biophysics Core

Circular Dichroism. This technique measures the overall secondary structure content

  • f proteins and quantifies changes in structure under different conditions.

Equipment: Jasco J-810 Spectropolarimeter with Peltier temperature control, automated dual titration system, and scanning emission monochromator Location: W8620

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Environmental Surveillance Core Spatial Science Core

Facility Co-Director

Timothy Shields, MA Department of Epidemiology Email: tshields@jhu.edu

Facility Co-Director

Frank Curriero, PhD Department of Epidemiology Email: fcurriero@jhu.edu

Environmental Surveillance Core Facility Johns Hopkins Bloomberg School of Public Health 615 N. Wolfe Street, Room E3025 Baltimore, Maryland 21205 Telephone: 410-502-9077 fax: 410-955-0105

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Overview - Spatial Analysis

Rationale

Identified malaria outcomes and measured environmental determinants likely vary spatially. Collect and harness this information to improve

  • study design
  • analysis
  • prevention, intervention, control and policy efforts

Obtain, Collect, Create Images (Satellite, Google Earth) Spatial Data Accuracy Non-spatial data Maps Spatial Database/Integration View Patterns Simultaneously Generate/Refine Hypothesis Quantify Relationships Test Hypothesis Study Design Go Beyond the Map!

Spatial Data GIS* Spatial Statistics

*Geographic Information System

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Spatial Data

  • Satellite Imagery
  • Geography, Hydrology
  • Land Use/Land Cover
  • Climate, Census
  • GPS’d locations
  • Outcome locations
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New Analysis Opportunities

Integration, Hypothesis Generation

GIS

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Spatial Statistics

  • Risk map estimation
  • Identify Env factors
  • Assess clustering
  • Trends over space & time
  • Detect clusters
  • Movement over space & time

Choma Mutasa Nchelenge

Rainy Dry

Gambiae Counts GPS Logged Movement

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DESCRIPTION

  • Location : SPH 4th floor, Room

W4700

  • Size: 3000 sq ft. divided into

two operational areas – High (Area 2) and Low containment (Areas 1, 3 & 4).

  • There are seven procedure

rooms and an autoclave.

INSECTARY MAP

The JHMRI Insect Core Facility

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  • 7 environmentally controlled

walk-in incubators for mosquito rearing.

  • 10 reach-in incubators whose

temperature and humidity can be custom adjusted.

Walk-in incubator for mosquito rearing

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Services

  • Mosquito rearing : An gambiae

and An. stephensi

  • Provide space and assistance for

rearing of other mosquito species.

  • Training: insectary use,

mosquito rearing, handling and identification techniques.

  • Mosquito transformation

(transgenic mosquitoes)

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For new insectary users:

  • Need orientation training by insectary manager before access

to the insectary is granted.

  • Need specialized training to work with Plasmodium

falciparum-infected mosquitoes.

  • Log in to iLabs to place mosquito orders.

https://johnshopkins.corefacilities.org/service_center/show_ex ternal/3806?name=malaria-mosquito-parasite-core

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Contacts

  • Facility Director:
  • Dr. Marcelo Jacobs-Lorena

School of Public Health Rm E4632 Tel: 443-287-0839 Email: ljacob13@jhu.edu

  • Facility Manager:
  • Mr. Christopher Kizito

School of Public Health Rm W4008 Tel: 443-287-0789 Email: ckizito1@jhu.edu

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http://www.parasitecore.org/

Facility Director:

George Dimopoulos, PhD, MBA gdimopo1@jhu.edu

Facility Co-Manager:

Godfree Mlambo, PhD gmlambo1@jhu.edu Facility Co-Manager: Abhai K. Tripathi, PhD atripat2@jhu.edu

Parasite Core Facility Johns Hopkins Bloomberg School of Public Health 615 N. Wolfe Street, Room W4212 Baltimore, Maryland 21205 Telephone: 410-502-7744 fax: 410-955-0105

Parasite Core Facility

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Parasite Core Facility

  • TISSUE/CELL/PARASITE CULTURE ROOM W4214
  • OFFICE AND MOLECULAR LAB ON 5TH FLOOR, W5315

Location

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Parasite Core Facility https://johnshopkins.corefacilities.org

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Parasite Core Facility

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Parasite Core Facility Custom Services or Collaboration Opportunities

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  • P. falciparum asexual inhibition assay
  • P. falciparum gametocytocidal assay
  • Transmission blocking assay (Membrane Feeding Assay)
  • P. berghei Ookinete development inhibition assay
  • In vivo drug efficacy assay in rodent model (Blood stage

challenge and Sporozoite challenge)

  • Generation of Transgenic parasite lines
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Genomic Analysis and Sequencing Core Facility

Genomic Analysis and Sequencing Core Facility Johns Hopkins Bloomberg School of Public Health 615 N. Wolfe Street, Room E4208 Baltimore, Maryland 21205 Telephone: 443-287-5967 fax: 410-955-0105

Facility Director:

Andrew Pekosz, PhD Email: apekosz1@jhu.edu

Facility Manager:

Anne Jedlicka, MS Email: ajedlic1@jhu.edu Amanda Dziedzic, MS Email: adziedz1@jhu.edu

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Core Services

  • Microarray experiments (Agilent, Affymetrix, Custom)
  • Next Generation Sequencing Library Prep (Illumina)
  • RNA-seq
  • DNA-seq (Genomic, Metagenomic, Targeted)
  • Genotyping studies (Affymetrix SNP, TaqMan allelic discrimination)
  • Real-Time PCR assays (TaqMan, PCRarray)
  • DNA and RNA purification and Quality Assessment
  • Detailed Data Analysis (microarray, qPCR, genotyping)
  • Consultation on experimental design, implementation, and/or analysis
  • Protocol adaptation and development
  • Instrument and Applications training (qPCR, imagers, etc)
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Recent JHMRI projects

  • Agilent Gene Expression Microarrays (Extraction through Analysis)
  • Mouse/Human (Stins)
  • Custom P. falciparum (Prigge)
  • Custom P. berghei (Sullivan)
  • Next-Gen Sequencing (Targeted)
  • Amplicons (Norris)
  • Sequence-capture, genomic
  • Parasite quant qPCR assays (Sinnis, Dinglasan)
  • Instruments and Software
  • Agilent SureScan Microarray Scanner (JHMRI)
  • ABI StepOne Plus Systems (MMI/JHMRI)
  • Partek Genomic Suite Software (MMI)
  • Illumina MiSeq (JHMI Biological Chemistry Core)
  • Illumina HiSeq 2500’s (JHMI High Throughput Seq Core)
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Imaging and Microscopy Resource Facility

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http://jhmmi.jhsph.edu/department/microscope1/FrameForm.cfm

Facility Director:

Isabelle Coppens, PhD Email: icoppen1@jhu.edu

Deconvolution/TIRF Microscope:

Anne Hamacher-Brady, PhD Email: abrady9@jhu.edu

Imaging and Microscopy Resource Facility Johns Hopkins Bloomberg School of Public Health 615 N. Wolfe Street, Room E2214 Baltimore, Maryland 21205 Telephone: 443-287-1589 fax: 410-955-0105

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Facility Services

The facility provides advanced instrumentation for the digital documentation

  • f everything from macromolecules to cells and tissues
  • Available Equipment
  • Nikon Eclipse E800 upright microscope
  • Nikon TE200 inverted microscope
  • Nikon Eclipse90i upright microscope
  • Zeiss AxioImager M2 upright microscope
  • New Users must first register and pass a quizz before gaining access to the facility

microscopes

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with automated systems with internal z motors, equipped with a videomicroscopy system and Volocity software

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DeltaVision Elite Deconvolution/TIRF microscope system (Installed: 6/16/2016)

Wide-field, fluorescence 3D deconvolution (image restoration) system, with a solid state illumination system and 488nm and 561nm laser lines. Deconvolution is an algorithm uses the point spread function (diffraction pattern) to deblur and remap out-of-focus fluorescent light, improving image resolution and contrast. Long-term live cell imaging experiments possible with incubation system with temperature control and an Ultimate Focus laser that monitors the position of the stage to eliminate z-drift during time-lapse studies. Further, precision stage allows continuous monitoring of multiple fields of view over extended periods of time. Advanced applications : TIRF (total internal reflection microscopy), a technique used to image samples within ~100-200nm of the coverslip surface, useful for cell surface, single molecule imaging (e.g. receptor mediated endocytosis). Photoactivation (PA), Fluorescent Recovery After Photobleaching (FRAP), and Fluorescent Loss in Photobleaching (FLIP), for investigating intracellular dynamics, such as the kinetics of protein diffusion and organelle interactions

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BD LSR II BD FACSCalibur BD FACSAria Cell Sorter MSD SECTOR Imager 2400 CTL ImmunoSpot

The Becton Dickinson Immune Function Laboratory and Cell Sorting Core

Faculty Co-Directors Jay Bream, Ph.D. jbream1@jhu.edu Joe Margolick, M.D., Ph.D. jmargol1@jhu.edu

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Interdisciplinary Research in Immunology and Beyond

  • Biological correlates

Vaccines/interventions Disease association

  • Soluble Biomarkers

cytokines, chemokines, inflammatory markers high sensitivity, multiplex, small volume, multiple sample types

  • Cell-based assays

cell distribution, activation, function, sorting, cell survival, signaling, cell cycle, proliferation, ect… T, B cells, innate immunity

  • We can help!

Study design, data acquisition, analysis, interpretation Training and/or collaboration No experience necessary! Tricia Nilles, Manager (2-9290) tnilles1@jhu.edu

Instrumentation:

  • BD FACSCalibur
  • BD LSR II
  • BD FACSAria Cell Sorter
  • MSD SECTOR Imager
  • CTL ImmunoSpot
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Useful Information

Reimbursements Send reimbursement requests approved by PI to Thom Hitzelberger at MMI-AP@jhu.edu Printing Posters Send requests to Thom at thitzel1@jhu.edu or to Tim Shields at tshields@jhu.edu Color Printers Request access to color printers in E5003 & E5651 Packages/FedEx

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Travel Funds

  • Matching PI funds up to $600 per academic year
  • For JHMRI Pre- and Post-docs to attend a conference to present

a poster or to give a talk

  • PI approval required
  • Submit request to Trish Ward

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MMI Travel Funds

  • 1st year MMI students are eligible for $500 per academic year
  • Submit request to Thom Hitzelberger

JHMRI Matching Travel Funds

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JHMRI Fellowships for Trainees

Maximum two years of support for malaria research Eligibility

  • Currently in a JHMRI laboratory
  • Pre-docs must be a PhD candidate, have completed all

coursework, lab rotations and departmental/school examinations including oral exam Letter of Intent due November 1; Application due December 1 http://malaria.jhsph.edu/opportunities/fellowships/

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JHMRI Pilot Grants for Faculty

Development of new malaria research ideas to lead to future external funding $150,000 maximum support over two years Pre-applications due May 1st Favorably reviewed pre-applicants are invited to apply http://malaria.jhsph.edu/opportunities/pilot-grants/

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malaria.jhsph.edu/

Conferences & Seminars

Future of Malaria Research Symposium in Rockville, MD

Oct/Nov

World Malaria Day Symposium

April 25

Vector Encounter

June

Malaria Friday Seminars

Sept - May

MMI/MRI Bulletin Board

  • pposite the MMI suite E5132
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Points of Contact

  • Trish Ward, trish.ward@jhu.edu

Room E5143, 443-287-8745 Items related to Dr. Agre, Conferences, MRI Travel Funds

  • Genevieve Williams, genevieve.williams@jhu.edu

Room E5141, 410-614-4883 Seminars, Pilot Grants, Fellowships, Conferences, Website, Listserve, and Macha, Zambia items

  • Meredith Piplani, meredith.piplani@jhu.edu

Room E5141, 410-614-4883 JHMRI Budget Items

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  • Thom Hitzelberger, thitzel1@jhu.edu

Room E5004, 443-287-5148 Reimbursements, MMI Travel Funds, Printers/Copiers, Poster Printing

  • Leonid Shats, lshats1@jhu.edu

Room E1305 (restricted access), 410-502-0510 Lab Equipment Training, Orientation, Safety, Room Access, and Equipment Maintenance

  • Lawanda Lewis, llewis36@jhu.edu

Room E5014, 443-287-4775 Human Resources and Payroll

  • Gail O’Connor, gail@jhu.edu

Room E5008, 410-614-4232 Academic advice and advocacy, BSPH procedures, student records, and MMI student recruitment

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Library Informationists

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Peggy Gross, peggy.gross@jhmi.edu Health Informationist, Welch Medical Library Room E5640, 410-502-7574 MMI Office Hours: Tuesdays 10:30am - 3:00 pm Rob Wright, rwrigh32@jhmi.edu Basic Science Informationist, Welch Medical Library SPH E5640/ (410) 502-6811 MMI Office Hours: Thursdays and Fridays, 10am-3pm Graduate student support prezi: https://bit.ly/2MHk8Z6

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JHMRI Friday Lunches w ith Invited Guest Speakers

Where: E5130 When: Fridays at noon when there is an invited guest speaker. Be sure to sign up via the link that is emailed at the beginning of the week!

Tori Balta Sullivan Lab: W4612 Dorian Jackson McMeniman Lab: W4104-3

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MMI Diversity & Inclusion Committee

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Dorian Jackson McMeniman Lab: W4104-3 djack102@jhmi.edu

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Student Research Group

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MMI Organizer: Madhura Kulkarni MMI Department PhD Candidate MMI Department Student Group President mkulkar5@jhmi.edu 978-758-4765

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MMI/JHMRI Postdoc Organization

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Postdoc Representative:

Genevieve Tauxe McMeniman Lab Email: gtauxe1@jhu.edu

Social event Coordinator:

Krithika Rajaram Prigge Lab Email: krajaram@jhu.edu

MMI Faculty Liaison:

Douglas Norris Douglas.Norris@jhu.edu

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Postdoc-focused events

  • MMI (and BMB) Postdoc Summer Seminar Series

– Weekly seminar series for postdocs to present their work

  • Quarterly Happy Hours with MMI Faculty

– Unwind and engage with faculty members

  • Informal monthly lunch hour with MMI and MRI postdocs

– Share your successes and woes, discuss events in the department and plan social gatherings. Looking for volunteer to lead meetings

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Resources and Communities for Postdocs

  • Hopkins Postdocs @ SPH
  • Hopkins Postdoctoral Association (JHPDA) https://jhpda.jhmi.edu
  • Professional Development and Career Office (PDCO)

https://pdco.med.jhmi.edu

  • Valeria Culotta: Provost’s Fellow and SPH Director of Postdoctoral

Training (vculott1@jhu.edu)

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Register today!

malaria.jhsph.edu