Phase III Flight Safety CGBA PGBA-04 Payload PGBA BioServe Space - - PowerPoint PPT Presentation

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Phase III Flight Safety CGBA PGBA-04 Payload PGBA BioServe Space - - PowerPoint PPT Presentation

Dec 04, 2023 181 likes •529 views

Phase III Flight Safety CGBA PGBA-04 Payload PGBA BioServe Space Technologies A Life Sciences NASA Commercial Space Center Alex Hoehn Assoc. Director Engineering / Safety, BioServe Sherry Woodard Engineering / Safety, BioServe BioServe

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CGBA + PGBA Phase 3 (STS-110, STS-112)

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Phase III Flight Safety PGBA-04 Payload BioServe Space Technologies A Life Sciences – NASA Commercial Space Center

Alex Hoehn

  • Assoc. Director Engineering / Safety, BioServe

Sherry Woodard Engineering / Safety, BioServe

BioServe Space Technologies, 429, UCB, Boulder, CO 80309-0429, Tel. (303) 492-1005, Fax (303) 492-8883

CGBA PGBA

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CGBA + PGBA Phase 3 (STS-110, STS-112)

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Acronyms

Gas Exchange (employs hydrophobic membrane for liquid containment). GE Group Activation Pack. GAP Isothermal Containment Module – a modular incubator for CGBA payload. ICM Internal Motor – Group Activation Pack. IM-GAP Failure Review Board. FRB Express Rack. ER Liquid Crystal Display, used in PGBA. LCD EXpedite the PRocessing of Experiments to Space Station. EXPRESS Electro-luminescent Display (yellow on back background), used in CGBA. EL-Display Emergency Warning and Caution (0=nominal, 1=Advisory, 3=Warning=Shutdown). EWAC / ECW Fluid Processing Apparatus. FPA Plant Generic BioProcessing Apparatus – a spaceflight greenhouse. PGBA End of Mission in GMT time (expected Shuttle landing time). EOM Commercial Generic BioProcessing Apparatus – payload name, uses ICM. CGBA

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CGBA + PGBA Phase 3 (STS-110, STS-112)

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PGBA-4 – STS-112 / ISS.9A Payload Overview PGBA-4 / STS-112 – ISS.9A -> ISS.11A – Inc.5 :

  • Science Summary.
  • Reflight (4th) of PGBA, first ISS flight.
  • Modifications since STS-94.

» Split coolant system / dual pump. » Modified nutrient delivery for timed germination.

Plant Access on Orbit:

  • Reverification of plant chamber seal.

Re-baseline PGBA hazard reports:

  • Generic HR now in STD-PGBA (JSC-1230).
  • Updated unique HR to latest ISS references.
  • Review of Hazard Reports.
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CGBA + PGBA Phase 3 (STS-110, STS-112)

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Science Overview Investigate time course of genetic changes due to microgravity exposure: 30 Arabidopsis thaliana plants:

Biologically simple (mustard-type plant). Fast growth rate – matures in 4-6 weeks. Entire genome sequenced.

Two crops during Inc.5, automated, time-

staggered germination, some germinated pre-launch:

1st crop sown pre-flight, multiple automated

germinations, single harvest, fixed on-orbit.

2nd crop sown in-flight, automated germination,

fixed on ground post-flight.

Mature plant, 4-6 weeks. Plant as harvested from PGBA, 3 weeks.

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CGBA + PGBA Phase 3 (STS-110, STS-112)

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PGBA Payload Overview

PGBA-03: STS-94 in Proto-EXPRESS / Spacelab. PGBA-04: Mufflers for ISS.

STS-77: 10 day mission STS-83: 4 day aborted MSL-1 STS-94: 16 day MSL-1 No PGBA anomalies,but:

  • transfer issues / lesson learned
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CGBA + PGBA Phase 3 (STS-110, STS-112)

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PGBA Faceplate with mufflers (ISS) 4 air inlets on inlet muffler. Mufflers attached with Velcro. 1 air outlet on exhaust muffler. Standard EXPRESS power and data interfaces. 4 standard NSTS locker latches.

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CGBA + PGBA Phase 3 (STS-110, STS-112)

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PGBA Faceplate without mufflers (NSTS) LCD display with touch screen, which can be enabled / disabled.

Enabled: Touch screen responsive. Disabled: Touch screen will not respond.

Air inlet screen. Air outlet Standard NSTS locker latches with modified handles.

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CGBA + PGBA Phase 3 (STS-110, STS-112)

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PGBA Connectors & Switches

Standard EXPRESS power and data interfaces. Mandatory tethered connector caps with Velcro. Main Power circuit breaker / LED and touch screen enable switch (guarded). Brace for Air Inlet muffler (Velcro attachment).

PFE Access

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CGBA + PGBA Phase 3 (STS-110, STS-112)

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PGBA Installation / GSE Equipment

  • Red eyebolts for Spacehab Tunnel Installation
  • GSE handles for transport / overhead installation

(captive fasteners not shown).

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CGBA + PGBA Phase 3 (STS-110, STS-112)

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Transfer Operations: PGBA to / from ISS

  • Powered transfer to maintain PGBA

environmental control.

  • Limit to 60 minutes power off time.
  • Locker replacement unit requires 8 captive

fastener for mounting (generic ISS tools).

  • Changes since MSL-1:
  • Captive fasteners / radial float on PGBA.
  • PMPs remain in middeck.
  • No tether requirement for rapid safing.
  • Assume: transfers now ‘routine’.
  • PD does not train astronauts on transfer
  • perations.

PGBA Transfer: Middeck to Spacelab, STS-94.

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CGBA + PGBA Phase 3 (STS-110, STS-112)

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ISS Operations – Status Check / Maintenance

CGBA (ER#4, position 3):

  • √ Power LED On ?
  • √ Airflow at Exhaust ?
  • √ Set Temp = Actual Temp ?
  • √ Time Incrementing ?
  • Maintenance: Clean inlet screen on muffler.

(Mufflers remain attached)

Status Check per Cue Card:

PGBA (ER#4, position 7/8) :

  • √ Power LED On ?
  • √ Airflow at Exhaust ?
  • √ EWAC=0 ?
  • √ Time Incrementing ?
  • Maintenance: Clean inlet screens

(4) on muffler. (Mufflers remain attached)

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CGBA + PGBA Phase 3 (STS-110, STS-112)

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Operations: Open PGBA Faceplate

  • 4 locker latches open.
  • Faceplate rotated around bottom edge

(hinged). Plant Chamber lid still closed: Inspect for free water.

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CGBA + PGBA Phase 3 (STS-110, STS-112)

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Remove Plant Chamber Lid Screws

10 captive fasteners (1-2 turns). Pull lid uniformly (parallel) from chamber.

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Harvesting Plant

Reach in, rotate each insert 2-3 turns ccw, then remove insert with plant. Repeat for all 30 plants, fix and store in CGBA. Insert new seed plug, rotate cw until tight.

Harvesting / Insertion of new seed plug (30x) into PGBA.

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ISS Operations – Harvesting

  • Remove fixative tube from CGBA, harvest

plant, place plant in tube, attach plunger rod, inject fixative - slowly, store in CGBA.

  • Repeat for entire PGBA crop 30x.

Plunger rod – connect after plant insertion

Fixative Tube

End cap – remove to insert plant

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CGBA + PGBA Phase 3 (STS-110, STS-112)

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Operations – Sowing

  • Place new dry seed plugs into PGBA root tray,
  • ne at a time (30x).
  • Close chamber lid, hand-tighten 10 captive

fasteners 2-3 times to ensure good seal.

  • Close PGBA faceplate, aligning pins and locking

latches (4x).

  • Reconnect cables, power up PGBA.
  • Replace mufflers.
  • Perform PGBA Status Check.

Felt

Removable Plug

Plastic Covered Steel Wire Small Plastic Centrifuge Tube Plastic Line

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CGBA + PGBA Phase 3 (STS-110, STS-112)

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Re-Attach and Re-seal Plant Chamber Lid

Attach chamber lid and push down. Tighten 10 captive fasteners (1-2 turns) going around perimeter until finger-tight. Finger-tighten 1-2 more times to ensure proper seal. (Optional battery-powered screw driver with Phillips head bit.)

Tighten screws 2-3 times around 1 2 3 4 5 2-3 times

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PGBA Plant Chamber

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Atmosphere Treatment System

CO2 O2 Hydrophobic Filter Return to Cabin Intake from Cabin Hydrophobic Filter CO2 O2 Delta-P Delta-P BaOH Scrubber Activated Carbon Filter PGC Return Intake from PGC Sealed Plant Growth Chamber C2H4 Scrubber

CO

2

O

2

Light H

2O

Pressure Temperature Relative Humidity Mixing Circulation

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CGBA + PGBA Phase 3 (STS-110, STS-112)

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Plant Growth Chamber - Intentionally Vented Container

CO2 O2 Hydrophobic Filter Return to Cabin Intake from Cabin Hydrophobic Filter CO2 O2 Delta-P Delta-P BaOH Scrubber Activated Carbon Filter PGC Return Intake from PGC Sealed Plant Growth Chamber C2H4 Scrubber

  • Max. Differential Pressure across one (1) Relief Valve

0.0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 10 20 30 40 50 60 Time [sec]

  • Max. Trans-Membrane Diff. Pressure [psid]
  • 24 psi/min
  • 9 psi/min

40 liter plant chamber volume, Circle Seal 259-2PP Relief Valve, 0.3 psid

0.567 psid @ -24psi/min 0.398 psid @ -9psi/min

Water trap

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CGBA + PGBA Phase 3 (STS-110, STS-112)

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Atmosphere Treatment System

CO2 O2 Hydrophobic Filter Return to Cabin Intake from Cabin Hydrophobic Filter CO2 O2 Delta-P Delta-P BaOH Scrubber Activated Carbon Filter PGC Return Intake from PGC Sealed Plant Growth Chamber C2H4 Scrubber

Atmosphere Treatment Cartridge:

  • Barium Hydroxide
  • Activated Charcoal

3 levels of containment:

  • Stainless steel frit
  • Glass mesh
  • Stainless steel screen
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CGBA + PGBA Phase 3 (STS-110, STS-112)

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Humidity Control / Condensate Recovery

Root Pack Removable Lid Root Pack Bottom Soil Needle Rubber Septa Base Comb Root Pack Removable Lid Root Pack Bottom Soil Needle Rubber Septa Base Comb

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Electric Subsystem

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Data Acquisition and Control

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Shock Hazard / Power Distribution

LCD Backlighting LCD Backlighting

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Thermal Subsystem

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Pressurized Systems – Atmosphere Treatment System

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Pressurized Systems – Temperature Control Loop

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Pressurized Systems – Humidity Control Loop

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Pressurized Systems – Nutrient Delivery System

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On-orbit Plant Access

  • Plants are non-hazardous.
  • Hardware failure resulting in free water know prior to crew

access, or visible prior to lid opening.

  • Lid re-sealing only necessary if:

» Humidity control system would subsequently fail and free condensate is created. » Nutrient Delivery System malfunctions and delivers excess water to single bag rather

than distributed bag (bags can hold all water if uniformly delivered).

  • If 50% of screws are sealed in a symmetric pattern, lid is water

tight at MDP.

  • Computer verifies if lid is attached and chamber can be

pressurized to relief valve opening pressure.

» ECW = 1 if lid seal not established.

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PGBA “Old” Hazard Reports

HR Title STS-112 Status

PGBA-1 Sharp Edges deleted, see new STD-PGBA, item 04, updated. PGBA-2 Structural Failure due to Launch,… updated; PGBA is a dual replacement locker. PGBA-3 Frangible Materials deleted, see new STD-PGBA, item 05, updated. PGBA-4 Rotating Devices deleted, see new STD-PGBA, item 13, updated. PGBA-5 Toxic Offgassing deleted, see new STD-PGBA, item 07, updated. PGBA-6 Flammable Materials deleted, see new STD-PGBA, item 06, updated. PGBA-7 Leakage of Fluid Samples updated for on-orbit crew access / harvesting. PGBA-8 Temperature Extremes deleted, see new STD-PGBA, item 10, updated. PGBA-9 Ignition Sources / Electrical deleted, see new STD-PGBA, item 11, updated. PGBA-10 Electric Shock updated, PGBA contains high voltage AC sources. PGBA-11 Rupture/Leakage Batteries updated, PGBA battery doesn’t meet JSC form 1230- item 9. PGBA-12 EMI deleted, see new STD-PGBA, item 08, updated. PGBA-13 Pressurized System Plant Chamber: vented container, see STD-PGBA, item 03. Liquid Coolant System, updated.

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CGBA + PGBA Phase 3 (STS-110, STS-112)

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PGBA “New” Hazard Reports

HR Title STS-112 Status

STD- PGBA Generic PGBA Hazard Controls New – derived from individual hazard reports from STS-77. PGBA-2 Structural Failure due to Launch,… Updated; PGBA is a dual replacement locker. PGBA-7 Leakage of Fluid Samples Updated; for on-orbit crew access / harvesting. PGBA-10 Electric Shock updated; PGBA contains high voltage AC sources. PGBA-11 Rupture/Leakage Batteries updated; PGBA battery doesn’t meet JSC form 1230-item 9. PGBA-13 Pressurized System Plant Chamber: vented container, see STD-PGBA, item 03. Liquid Coolant System, updated.