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Nerolidol-mediated rendezvous of dynastid beetles in long melon flowers
Arodi P. Favaris; Amanda C. Túler; Weliton D. Silva; Walter S. Leal; José Maurício S. Bento
Lagenaria siceraria (Curcubitaceae)
Nerolidol-mediated rendezvous of dynastid beetles in long melon - - PowerPoint PPT Presentation
Nerolidol-mediated rendezvous of dynastid beetles in long melon - - PowerPoint PPT Presentation
2018 ESA,ESC and ESBC joint Annual Meeting Nerolidol-mediated rendezvous of dynastid beetles in long melon flowers Arodi P. Favaris; Amanda C. Tler; Weliton D. Silva; Walter S. Leal; Jos Maurcio S. Bento Arod Prado Favaris Lagenaria
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Lagenaria siceraria (Curcubitaceae) Long melon/Bottle gourd plant
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Cyclocephala paraguayensis (Coleoptera, Melolonthidae, Dynastinae)
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The genus Cyclocephala (tribe Cyclocephalini)
Gottsberger, Pl.Syst.Evol. (1986)
Beetle flower association
flower attraction
aggregation mating
- pportunity
volatiles
food resource shelter
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The genus Cyclocephala (tribe Cyclocephalini)
Gottsberger, Pl.Syst.Evol. (1986)
Beetle flower association
flower attraction
aggregation mating
- pportunity
volatiles
food resource shelter
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The genus Cyclocephala (tribe Cyclocephalini)
Gottsberger, Pl.Syst.Evol. (1986)
Beetle flower association
flower attraction
aggregation mating
- pportunity
volatiles
food resource shelter
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Do long melon flower volatiles attract Cyclocephala paraguayensis?
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Flower volatile collection
battery vaccum pump / adsorbent polymer
GC-EAD Field trial
Filter paper ‘flowers’ Treated: 100 μL synthetic compound Control: no compound
Chemical identification
GC-MS/ synthetic standard
Methods
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GC-EAD Field trial
Filter paper ‘flowers’ Treated: 100 μL synthetic compound Control: no compound
Chemical identification
GC-MS/ synthetic standard
Methods
Flower volatile collection
battery vaccum pump / adsorbent polymer
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Flower volatile collection
battery vaccum pump / adsorbent polymer
GC-EAD Field trial
Filter paper ‘flowers’ Treated: 100 μL synthetic compound Control: no compound
Chemical identification
GC-MS/ synthetic standard
Methods
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Flower volatile collection
battery vaccum pump / adsorbent polymer
GC-EAD Field trial
Filter paper ‘flowers’ Treated: 100 μL synthetic compound Control: no compound
Chemical identification
GC-MS/ synthetic standard
Methods
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Results
GC-EAD
♂ ♀
aeration extract
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Results
GC-EAD
♂ ♀
aeration extract
30 40 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 210 220 230 240 250 260 270 280 25 50 75 100 % 69 93 41 107 43 67 119 136 161 148 189 204 175 222 252 241 279 271Chemical identification: GC-MS
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Results
GC-EAD
♂ ♀
aeration extract
30 40 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 210 220 230 240 250 260 270 280 25 50 75 100 % 69 93 41 107 43 67 119 136 161 148 189 204 175 222 252 241 279 271Trans-nerolidol Chemical identification: GC-MS
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Results
GC-EAD
♂ ♀
aeration extract
30 40 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 210 220 230 240 250 260 270 280 25 50 75 100 % 69 93 41 107 43 67 119 136 161 148 189 204 175 222 252 241 279 271Trans-nerolidol Chemical identification: GC-MS Insect total catches: Treated: 6 Control: 0
Exact binomial test of goodness-
- f-fit (P=0.03)
Field trial
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Field experiment (Cassilândia, Brazil)
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Exact binomial test of goodness-of-fit (P<0.01)
Field experiment (Cassilândia, Brazil)
280 50 100 150 200 250 300
NEROLIDOL CONTROL
Total number of insects/treatment
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Conclusion
Nerolidol contributes to Cyclocephala paraguayensis (Coleoptera: Melolonthidae) aggregation in long melon flowers, Lagenaria siceraria (Curcubitaceae)
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