Lyon INSA Team SIX BILLION DOLLARS SIX BILLION DOLLARS 24 000 - - PowerPoint PPT Presentation

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Lyon INSA Team SIX BILLION DOLLARS SIX BILLION DOLLARS 24 000 - - PowerPoint PPT Presentation

May 26, 2023 198 likes •624 views

Lyon INSA Team SIX BILLION DOLLARS SIX BILLION DOLLARS 24 000 Ferraris ! SIX BILLION DOLLARS DISINFECTANTS WHY SO MUCH DISINFECTANT ? BIOFILM BIOFILMS : THE INDUSTRIAL ENEMY ! More pumping power / Pressure drops Source: N. Zelver,

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Lyon – INSA Team

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SIX BILLION DOLLARS

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SIX BILLION DOLLARS

24 000 Ferraris !

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SIX BILLION DOLLARS DISINFECTANTS

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WHY SO MUCH DISINFECTANT ? BIOFILM

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BIOFILMS : THE INDUSTRIAL ENEMY !

More pumping power / Pressure drops

A boom stretches out to contain the pipeline leak. A pipeline leak spews oil into a central Alberta lake

Maintenance Leaks + Environmental Impact

Source : Calgary Herald Source : Daveberta.ca

Source: N. Zelver, from biofilmbook.com Source : “Microbiology control for oil and gas applications” June 2012, courtesy of Dow and Schlumberger

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BIOFILMS : THE INDUSTRIAL ENEMY !

More pumping power / Pressure drops Maintenance Leaks + Environmental Impact Human health

Source: N. Zelver, from biofilmbook.com Source : “Microbiology control for oil and gas applications” June 2012, courtesy of Dow and Schlumberger Source : Biomerieux

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Understand

  • Biofilm issues
  • Cleaning processes

Cosmetics and health industry Oil industry Manufacturer of enzyme cocktails

  • Industrial installations

MEETING WITH INDUSTRIALISTS

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EXISTING SOLUTIONS

Chemical

Toxic reagents

Biological

Enzymatic cocktails

Stabilization Physical

Not fully efficient

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  • Reduce the cost
  • Improve the efficiency
  • Be Environmentally friendly

OUR OBJECTIVES

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Project

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WEAPON

Bacillus

TARGET

Biocide agent Scattering agent

FIRST STEP : DESTROY THE BIOFILM

LYSOSTAPHIN DISPERSIN

  • S. aureus

biofilm

LYSOSTAPHIN BioBrick™ BBa_K802000

Lyso

RBSb

DISPERSIN BioBrick™ BBa_K802001

Disp

RBSb

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Context Project

Bacillus subtilis « TORPEDO »

Houry, Gohar, Deschamps, Tischenko, Aymerich, Gruss & Briandet, Proc Natl Acad Sci U S A, 2012.

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Biocide and scattering agents secreted

inside the biofilm and not above,

thanks to the swimming capacity of Bacillus. Bacillus subtilis « TORPEDO »

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LYSOSTAPHIN

CONTROL

CONFOCAL LASER SCANNING MICROSCOPY RESULTS

  • S. aureus biofilm

30 µm

  • S. aureus biofilm treated with
  • B. subtilis + Lysostaphin BioBrick™
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CONTROL

CONFOCAL LASER SCANNING MICROSCOPY RESULTS

  • S. aureus biofilm
  • S. aureus biofilm treated with
  • B. subtilis + Dispersin BioBrick™

DISPERSIN

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CONTROL

CONFOCAL LASER SCANNING MICROSCOPY RESULTS

  • S. aureus biofilm

LYSOSTAPHIN + DISPERSIN

BIOFILM ELIMINATION : 99 %

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CONCLUSION OF THE FIRST STEP

KILL MODULE !

LYSOSTAPHIN + DISPERSIN : BETTER RESULT, CLEANER SURFACE

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COAT

Option SECOND STEP

Avoid new biofilm formation

STICK

Option

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TWO OPTIONS : WHY ?

Chemical industry

COAT

Poultry and animal farming , Oil industry

STICK

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sfp Pxyl abrB

RBSb RBSb

lacI

RBSb

TOGGLE SWITCH SYSTEM

SURFACTIN + BIOFILM BioBricks™ BBa_K802009

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sfp lacI abrB

Pxyl

STICK (biofilm) No surfactant, No biofilm repressor xylR Repression

IPTG

STICK Option

Plac

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sfp lacI abrB Surfactant regulator

Pxyl

COAT (planktonic cells) Biofilm repressor

XYLOSE

xylR

Plac

COAT Option

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STICK RESULTS

D abrB Biofilm D abrB/BBa_K802009 No biofilm

abrB is functional in our part

sfp Pxyl abrB

RBSb RBSb

lacI

RBSb

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  • Protective B. Subtilis biofilm (red)
  • Colonization by E.coli (green)

The protective biofilm is efficient

STICK : CONFOCAL MICROSCOPY RESULTS PROTECTIVE BIOFILM

CONTROL

E.coli biofilm

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COAT RESULTS

Surfactin production

Positive control: LB + SDS Supernatant empty plasmid Supernatant BBa_K802009

sfp Pxyl abrB

RBSb RBSb

lacI

RBSb

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COAT : CONFOCAL MICROSCOPY RESULTS

CONTROL

E.coli biofilm established on Glass Slide

SURFACTIN

  • Surface treated with B. Subtilis supernatant

+ Surfactin BioBrick™.

  • Colonization by E. coli.

The surfactin pre-treatment is efficient

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CONCLUSION OF THE SECOND STEP

COAT & STICK OPTIONS :

Both efficient !!

The surface is protected

from a new biofilm formation

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Switch control

MODELLING : OBJECTIVES

Behavior anticipation

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MODELLING

Physiological response

Stick Coat

IPTG Concentration

Stick Coat

Xylose Concentration Physiological response

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HOW TO DEVELOP AN INDUSTRIAL SOLUTION ?

Meeting with industrialists Meeting with researchers

Develop an industrial process

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Meeting with researchers

François KEPES: SynBio pioneer in France. Romain BRIANDET : Leader of the

Bioadhesion, Biofilms and Surface Hygiene lab at AgroParisTech INRA Massy (Paris).

Organisation of conferences :

  • To improve our knowledge

...and to share this knowledge

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1: Add BACILLUS into the tank 2: Tank cleaning (4 hours cycle in the hydraulic system A) 3: Circuit cleaning (4 hours cycle in the hydraulic system B/C) 4: Rinsing 5: Biological waste recovery (0.45 and 0.2 µm filter) 6: Optional: Disinfection

  • r COAT / STICK options

Develop an industrial solution

1 5

FILTER

4 2 3 6

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A new economic model ? Our Reflexion

Economic issues for SynBio

  • Analyze economic theories
  • To propose a viable economic

system for iGEM and SynBio associating industrial partners

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THE RESEARCHER’S NIGHT Outreach local community with positive applications of SynBio

Debating with the public

PUBLIC OPINION WITH A SURVEY

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By debating with students and staff from different institutes Outreach University members

Computer scientist Economist Mechanical engineer Master students

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HUMAN PRACTICE GOALS

Economic issues for SynBio Debating with the public

Build a community

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First meeting initiated this year by Lyon

  • Synbio programmation by

Governmental agencies

  • Biosafety and bioethics
  • SynBio teaching
  • Synbio Research and

development

Promote and develop :

Paris Bettencourt Paris Saclay Evry Lyon INSA Grenoble Bordeaux

iGEM FRANCE

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First meeting initiated this year by Lyon

  • Synbio programmation by

Governmental agencies

  • Biosafety and bioethics
  • SynBio teaching
  • Synbio Research and

development

Promote and develop :

iGEM FRANCE

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Main Achievements

LYSOSTAPHIN BioBrick™ /BBa_K802000 DISPERSIN BioBrick™/BBa_K802001 SURFACTIN + BIOFILM / BBa_K802009 2 shuttle plasmids BBa_K802003 and BBa_K802004

 A proposition for an Economic System for Synthetic Biology

Collaboration with UBC

 A promising solution to control biofilms on closed surfaces  Characterization of new parts :

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Acknowledgments

TEACHING FACILITIES INSA And all scientific, technical and administrative staff from :

  • Biosciences Department of INSA Lyon
  • MAP and BF2I laboratories
  • MICALIS INRA laboratory

And our sponsors :