HOW TO FIND THE RIGHT TREATMENT, AT THE RIGHT TIME: MICROFLUIDICS - PowerPoint PPT Presentation

HOW TO FIND THE RIGHT TREATMENT, AT THE RIGHT TIME: MICROFLUIDICS CAN HELP! Prof. Gabriele Dubini | Politecnico di Milano MASTERCLASS SERIES 2020 VIRTUAL EXPERIENCE

DRUG SCREENING Drug screening is the process by which potential drugs are identified and optimized before selection of a candidate drug to progress to clinical trials Drug n°1 Drug n°2 … Drug n°50 Tumour Cells from In vitro tests !!! Cancer patients biopsy biopsy on EFFICACY 2 MASTERCLASS SERIES – 2020 VIRTUAL EXPERIENCE – PROF. GABRIELE DUBINI

DRUG SCREENING Single drug Drug n°1 Drug n°2 … Drug n°50 In vitro tests on EFFICACY 3 ‹N› MASTERCLASS SERIES – 2020 VIRTUAL EXPERIENCE – PROF. GABRIELE DUBINI

DRUG SCREENING X 10… X 100… X 1000… Single drug Comb. of 2 drugs Multiple combinations Drug n°1 Diff. timing of Drug n°2 administration … Drug n°50 Dosages … In vitro tests on EFFICACY 4 ‹N› MASTERCLASS SERIES – 2020 VIRTUAL EXPERIENCE – PROF. GABRIELE DUBINI

DRUG SCREENING X 10… Common laboratory practices X 100… !!! X 1000… They are Time consuming 5 ‹N› MASTERCLASS SERIES – 2020 VIRTUAL EXPERIENCE – PROF. GABRIELE DUBINI

DRUG SCREENING X 10… Common laboratory practices X 100… Automation for high-throughput management X 1000… Cannot manage efficiently jelly components such as biological matrices !!! Cannot directly manage any flow !!! 6 ‹N› MASTERCLASS SERIES – 2020 VIRTUAL EXPERIENCE – PROF. GABRIELE DUBINI

DRUG SCREENING To screen the efficacy of the treatment and build up -PATIENT SPECIFIC- models or predictions to overcome current limitations WHAT DO WE NEED? 7 ‹N› MASTERCLASS SERIES – 2020 VIRTUAL EXPERIENCE – PROF. GABRIELE DUBINI

DRUG SCREENING What do we need? A patient-specific tumor microenvironment Biological/genetic materials DRUG secreted by cells Flow OXYGEN FEEDING WASTE Multiple combinations biological matrices Diff. timing of !!! administration flow Dosages !!! 8 ‹N› MASTERCLASS SERIES – 2020 VIRTUAL EXPERIENCE – PROF. GABRIELE DUBINI

DRUG SCREENING Common laboratory practices High-throughput robots Microfluidics can help! 9 MASTERCLASS SERIES – 2020 VIRTUAL EXPERIENCE – PROF. GABRIELE DUBINI

DRUG SCREENING Common laboratory practices High-throughput robots Microfluidics can help! HOW? 10 MASTERCLASS SERIES – 2020 VIRTUAL EXPERIENCE – PROF. GABRIELE DUBINI

DRUG SCREENING Common laboratory practices High-throughput robots HOW? Microfluidics can help! 11 MASTERCLASS SERIES – 2020 VIRTUAL EXPERIENCE – PROF. GABRIELE DUBINI

DRUG SCREENING Common laboratory practices High-throughput robots HOW? Microfluidics can help! • Miniaturization • Reduction of experiment time • Reduction of overall costs • Improvement of experiment precision • Reduction of sample and reagent consumption 12 MASTERCLASS SERIES – 2020 VIRTUAL EXPERIENCE – PROF. GABRIELE DUBINI

MICROFLUIDICS Microfluidics is the science of fluids manipulation at the micron ( µm ) scale m Red blood cells cm mm Hair 8 µm µ m 13 MASTERCLASS SERIES – 2020 VIRTUAL EXPERIENCE – PROF. GABRIELE DUBINI

MICROFLUIDICS Microfluidics is the science of fluids manipulation at the micron ( µm ) scale Microfluidics can be found in nature WHAT IS BEHIND? 14 MASTERCLASS SERIES – 2020 VIRTUAL EXPERIENCE – PROF. GABRIELE DUBINI

MICROFLUIDICS Microfluidics is the science of fluids manipulation at the micron ( µm ) scale WHAT IS BEHIND? Surface tension Capillarity 15 MASTERCLASS SERIES – 2020 VIRTUAL EXPERIENCE – PROF. GABRIELE DUBINI

MICROFLUIDICS APPLICATIONS Glycaemic Lab-on-chips tests Drug delivery Point of care systems diagnostics EVERYDAY (e.g. insulin) (e.g. COVID19) LIFE & LAB TOOLS Analytical Pregnancy devices tests 16 MASTERCLASS SERIES – 2020 VIRTUAL EXPERIENCE – PROF. GABRIELE DUBINI

ACCELERATOR PROJECT ACCELERATOR AWARD A26819 Objectives : study of drug-resistance mechanism and identification of the best therapeutic treatments for drug-resistant patients using a novel microfluidic platform Cells from tumour biopsy Biological matrix 17 MASTERCLASS SERIES – 2020 VIRTUAL EXPERIENCE – PROF. GABRIELE DUBINI

MICROFLUIDIC PLATFORM DROPS FOR DRUG SCREENING Matrigel drop FEEDING Organoid OXYGEN Well DRUG Medium/Drug WASTE Biological/genetic materials 18 18 MASTERCLASS SERIES – 2020 VIRTUAL EXPERIENCE – PROF. GABRIELE DUBINI

MICROFLUIDIC PLATFORM - DROPS FOR DRUG SCREENING DROPS GENERATION in MICROCHANNELS Matrigel drop 19 19 MASTERCLASS SERIES – 2020 VIRTUAL EXPERIENCE – PROF. GABRIELE DUBINI

MICROFLUIDIC PLATFORM - DROPS FOR DRUG SCREENING Multicomponent FLOW around the drop DROPS GENERATION in MICROCHANNELS FEEDING OXYGEN DRUG WASTE Matrigel drop Biological/genetic materials 20 20 MASTERCLASS SERIES – 2020 VIRTUAL EXPERIENCE – PROF. GABRIELE DUBINI

MICROFLUIDIC PLATFORM DROPS FOR DRUG SCREENING 2 cm 21 21 MASTERCLASS SERIES – 2020 VIRTUAL EXPERIENCE – PROF. GABRIELE DUBINI

RESEARCH GROUP ACCELERATOR AWARD A26819 Nicola Valeri Gabriele Dubini Giovanni Tonon Elena Bianchi Paola De Stefano Oronzina Botrugno Assistant Professor PhD Candidate Post Doc 22 22 MASTERCLASS SERIES – 2020 VIRTUAL EXPERIENCE – PROF. GABRIELE DUBINI

ACKNOWLEDGEMENTS We thank DiCE for participating in the project and bringing the patient’s voice into research 23 23 MASTERCLASS SERIES – 2020 VIRTUAL EXPERIENCE – PROF. GABRIELE DUBINI

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