HOW TO FIND THE RIGHT TREATMENT, AT THE RIGHT TIME: MICROFLUIDICS - - PowerPoint PPT Presentation

• Prof. Gabriele Dubini | Politecnico di Milano

HOW TO FIND THE RIGHT TREATMENT, AT THE RIGHT TIME: MICROFLUIDICS CAN HELP!

MASTERCLASS SERIES 2020 VIRTUAL EXPERIENCE

MASTERCLASS SERIES – 2020 VIRTUAL EXPERIENCE – PROF. GABRIELE DUBINI

DRUG SCREENING

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Drug screening is the process by which potential drugs are identified and optimized before selection of a candidate drug to progress to clinical trials

Cells from biopsy Drug n°1 Drug n°2 Cancer patients Tumour biopsy Drug n°50

…

!!!

In vitro tests

• n EFFICACY

‹N›

MASTERCLASS SERIES – 2020 VIRTUAL EXPERIENCE – PROF. GABRIELE DUBINI

Single drug

Drug n°1 Drug n°2 Drug n°50 …

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In vitro tests

• n EFFICACY

DRUG SCREENING

‹N›

MASTERCLASS SERIES – 2020 VIRTUAL EXPERIENCE – PROF. GABRIELE DUBINI

• Comb. of 2 drugs

Single drug

Drug n°1 Drug n°2 Drug n°50 …

…

X 10… X 100… X 1000…

In vitro tests

• n EFFICACY

Multiple combinations Dosages

• Diff. timing of

administration

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DRUG SCREENING

‹N›

MASTERCLASS SERIES – 2020 VIRTUAL EXPERIENCE – PROF. GABRIELE DUBINI

Common laboratory practices

!!! X 10… X 100… X 1000…

They are Time consuming

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DRUG SCREENING

‹N›

MASTERCLASS SERIES – 2020 VIRTUAL EXPERIENCE – PROF. GABRIELE DUBINI

Common laboratory practices Automation for high-throughput management

Cannot manage efficiently jelly components such as biological matrices Cannot directly manage any flow

!!! !!!

X 10… X 100… X 1000…

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DRUG SCREENING

‹N›

MASTERCLASS SERIES – 2020 VIRTUAL EXPERIENCE – PROF. GABRIELE DUBINI

To screen the efficacy of the treatment and build up

• PATIENT SPECIFIC-

models or predictions to overcome current limitations

WHAT DO WE NEED?

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DRUG SCREENING

‹N›

MASTERCLASS SERIES – 2020 VIRTUAL EXPERIENCE – PROF. GABRIELE DUBINI

FEEDING OXYGEN

Flow

WASTE

biological matrices flow

!!! !!!

Multiple combinations

• Diff. timing of

administration Dosages A patient-specific tumor microenvironment

DRUG Biological/genetic materials secreted by cells

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What do we need?

DRUG SCREENING

MASTERCLASS SERIES – 2020 VIRTUAL EXPERIENCE – PROF. GABRIELE DUBINI

DRUG SCREENING

High-throughput robots Common laboratory practices

Microfluidics can help!

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MASTERCLASS SERIES – 2020 VIRTUAL EXPERIENCE – PROF. GABRIELE DUBINI

DRUG SCREENING

HOW?

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High-throughput robots Common laboratory practices

Microfluidics can help!

MASTERCLASS SERIES – 2020 VIRTUAL EXPERIENCE – PROF. GABRIELE DUBINI

DRUG SCREENING

High-throughput robots Common laboratory practices

Microfluidics can help!

HOW?

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MASTERCLASS SERIES – 2020 VIRTUAL EXPERIENCE – PROF. GABRIELE DUBINI

DRUG SCREENING

Microfluidics can help!

• Miniaturization
• Reduction of experiment time
• Reduction of overall costs
• Reduction of sample and reagent consumption
• Improvement of experiment precision

HOW?

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Common laboratory practices High-throughput robots

MASTERCLASS SERIES – 2020 VIRTUAL EXPERIENCE – PROF. GABRIELE DUBINI

m

MICROFLUIDICS

Microfluidics is the science of fluids manipulation at the micron (µm) scale

cm mm

13 Red blood cells

8 µm µm

Hair

MASTERCLASS SERIES – 2020 VIRTUAL EXPERIENCE – PROF. GABRIELE DUBINI

MICROFLUIDICS

Microfluidics is the science of fluids manipulation at the micron (µm) scale Microfluidics can be found in nature

WHAT IS BEHIND?

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MASTERCLASS SERIES – 2020 VIRTUAL EXPERIENCE – PROF. GABRIELE DUBINI

MICROFLUIDICS

Microfluidics is the science of fluids manipulation at the micron (µm) scale

WHAT IS BEHIND?

Capillarity

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Surface tension

MASTERCLASS SERIES – 2020 VIRTUAL EXPERIENCE – PROF. GABRIELE DUBINI

MICROFLUIDICS APPLICATIONS

Point of care diagnostics (e.g. COVID19) Lab-on-chips Glycaemic tests Pregnancy tests Drug delivery systems (e.g. insulin) Analytical devices

EVERYDAY LIFE & LAB TOOLS

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MASTERCLASS SERIES – 2020 VIRTUAL EXPERIENCE – PROF. GABRIELE DUBINI

ACCELERATOR PROJECT

Objectives: study of drug-resistance mechanism and identification of the best therapeutic treatments for drug-resistant patients using a novel microfluidic platform

Biological matrix Cells from tumour biopsy

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ACCELERATOR AWARD A26819

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MASTERCLASS SERIES – 2020 VIRTUAL EXPERIENCE – PROF. GABRIELE DUBINI

Matrigel drop Organoid Well Medium/Drug 18

FEEDING OXYGEN WASTE DRUG Biological/genetic materials

DROPS FOR DRUG SCREENING

MICROFLUIDIC PLATFORM

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MASTERCLASS SERIES – 2020 VIRTUAL EXPERIENCE – PROF. GABRIELE DUBINI

MICROFLUIDIC PLATFORM - DROPS FOR DRUG SCREENING

Matrigel drop 19

DROPS GENERATION in MICROCHANNELS

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MASTERCLASS SERIES – 2020 VIRTUAL EXPERIENCE – PROF. GABRIELE DUBINI

MICROFLUIDIC PLATFORM - DROPS FOR DRUG SCREENING

Matrigel drop 20

FEEDING OXYGEN WASTE DRUG Biological/genetic materials

DROPS GENERATION in MICROCHANNELS Multicomponent FLOW around the drop

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MASTERCLASS SERIES – 2020 VIRTUAL EXPERIENCE – PROF. GABRIELE DUBINI

MICROFLUIDIC PLATFORM DROPS FOR DRUG SCREENING

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2 cm

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MASTERCLASS SERIES – 2020 VIRTUAL EXPERIENCE – PROF. GABRIELE DUBINI

Gabriele Dubini Elena Bianchi Assistant Professor Giovanni Tonon Paola De Stefano PhD Candidate

Nicola Valeri

Oronzina Botrugno Post Doc

RESEARCH GROUP

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ACCELERATOR AWARD A26819

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MASTERCLASS SERIES – 2020 VIRTUAL EXPERIENCE – PROF. GABRIELE DUBINI

ACKNOWLEDGEMENTS

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We thank DiCE for participating in the project and bringing the patient’s voice into research

THANK YOU