Green Synthesis of Gold and Silver Nanoparticles from Plant Extracts - - PowerPoint PPT Presentation

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Green Synthesis of Gold and Silver Nanoparticles from Plant Extracts - - PowerPoint PPT Presentation

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Green Synthesis of Gold and Silver Nanoparticles from Plant Extracts Cassandra Dyal, 1 Nguyen Nguyen, 1 Jodi Hadden, 1 Linfeng Gou, 2 Tan, Li, 2 Catherine J. Murphy, 2 Will Lynch, 1 Delana Nivens 1 1 Department of Chemistry and Physics, Armstrong

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Green Synthesis of Gold and Silver Nanoparticles from Plant Extracts

Cassandra Dyal,1 Nguyen Nguyen,1 Jodi Hadden,1 Linfeng Gou,2 Tan, Li,2 Catherine J. Murphy,2 Will Lynch,1 Delana Nivens1

1Department of Chemistry and Physics, Armstrong Atlantic

State University, 11935 Abercorn St., Savannah, GA 31419

2Department of Chemistry and Biochemistry, University of

South Carolina, Columbia, SC 29208

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Abstract

The synthesis of metal and semiconductor nanoparticles is an expanding research area due to the potential applications for the development of novel technologies. Generally, nanoparticles are prepared by a variety of chemical methods which are not environmentally friendly. We report a rapid and convenient method to reductively prepare gold and silver nanoparticles from auric chloride and silver nitrate respectively. In this report we use aqueous extracts from plants indigenous to southeastern Georgia, namely the Southern Live Oak (Quercus virginiana), Southern Magnolia (Magnolia grandiflora), Kudzu (Pueraria lobata) and Loblolly Pine (Pinus taeda). The extracts template the reductive preparation of both gold and silver nanoparticles which are found to be < 100 nm in size. The synthesis and characterization, including FT-IR, TGA and TEM analysis of these particles will be described.

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Purpose

Identify “green” pathways to produce nanoparticles using regionally (south Georgia) significant resources Presently, minimal information on using environmentally benign chemicals for particle synthesis

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SLIDE 4

Prepare aqueous extract – broth prepared by boiling 20 grams of plant leaf mass in 100 mL distilled water for 5 minutes followed by filtering. Broth stored in refrigerator at 4ºC. Use extracts from: Quercus virginiana – Southern Live Oak Magnolia grandiflora – Southern Magnolia Pueraria lobata – Kudzu Pinus taeda – Loblolly Pine Add 5 mLs of extract with 100 mLs of 1 mM AgNO3 or AuCl3 heated to 75ºC. Maintain this temperature for the entire reaction (different plant extracts require different times to complete the reaction) Monitor the UV-Vis spectrum of the particle formation (exciton band near 430 nm for silver and 550 nm for gold Centrifuge product, wash 3 x with di water and perform FT-IR, TGA and TEM analysis

Experimental

Gold Nanoparticles

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Ag Nanoparticle Formation Live Oak Magnolia

UV-Vis Spectra of the Formation of Silver Nanoparticles Using Live Oak Leaf Broth

0.5 1 1.5 2 2.5

200 300 400 500 600

Wavelength (nm)

Absorbance

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Au Nanoparticle Formation Live Oak Magnolia

0.5 1 1.5 300 400 500 600 700

Wavelength (nm) Absorbance

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Ag/Au Nanoparticle Formation – Other Plants

Pine (Cold) Pine (Hot) Kudzu

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Au Nanoparticle Formation – Temperature Dependence

Gold Nanoparticles Formation Rate

  • 0.50000

0.00000 0.50000 1.00000 1.50000 2.00000 4 8 12 16 20 Time (min) Absorbanc (Au) 40 60 23 80 70

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SLIDE 9

Au Nanoparticle Formation – Concentration Dependence

Gold Nanoparticles Formation Rate

0.00 0.50 4 8 12 16 20 Time (min) Absorbanc (AU) Room Temp. Room Temp. 2x Extract

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“Green” Nanoparticle IR Analysis Ag – Live Oak

76 77 78 79 80 400 1400 2400 3400 Wavenumber (cm-1) % T

2917 3351 3186 1613 1355 1044

Wavenumber (cm

  • 1) Functional group Biomolecule

1044 Alkane, C-O stretch Lipids 1613 Amide Amino acids 2917 Alkane C-H stretch Lipids 3186 Hydroxyl group Sugars 3351 N-H amines Amino acids

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“Green” Nanoparticle IR Analysis Au – Magnolia

15 25 35 45 55 500 1000 1500 2000 2500 3000 3500 4000 1627cm-1 1450 cm-1 1141 cm-1 2931 cm-1 3502cm-1

W a v e n u m b e r (cm

  • 1)

F u n ctio n a l g ro u p B io m

  • le

cu le 1 1 4 1 C

  • O

,C

  • N
  • r C

C S tre tc h L ip id s, A m in

  • a

cid s ,S u g a rs 1 4 5 C H , O H b e n d L ip id s, S u g a rs 1 6 2 7 A m id e A m in

  • a

cid s 2 9 3 1 A lka n e C

  • H

stre tch L ip id s 3 5 2 N

  • H

a m in e s A m in

  • a

cid s

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TGA Analysis

40 60 80 100 100 250 400 550 700 850 1000 Temperature (oC) Weight % 70 80 90 100 100 250 400 550 700 850 1000 Temperature (oC) Weight %

Scan Rate = 20 °C/min Live oak loss-28% Magnolia Loss 57% Gold - Magnolia Gold Live Oak

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TGA Analysis

55 70 85 100 100 250 400 550 700 850 1000 Temperature (oC) Weight %

Silver, Magnolia Loss of 44% Scan Rate = 40 °C/min

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Conclusions

  • 1. Green methods for NP preparation are being developed
  • 2. Pine and Live oak extracts seem to be the fastest for the

preparation of both gold and silver nanoparticles while Kudzu is the slowest.

  • 3. Rate of reaction is dependent upon T and [ Mn+ ]
  • 4. IR indicates biomolecule capping agent present
  • 5. Sizes are typically on the order of 20 nm by TEM imaging.
  • 6. Some interesting triangle shaped nanoparticles are seen in

the gold nanoparticles

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SLIDE 15

Acknowledgements

  • National Science Foundation Division of Undergraduate

Education: Nanotechnology in Undergraduate Education Grant CHE/NUE 0303994

  • National Science Foundation Course Curriculum and

Improvement Grant DUE/CCLI - 9952343

  • USG Matching Grant
  • AASU College of Arts and Sciences, Department of Chemistry

and Physics

  • AASU Research and Scholarship Grant / Gignilliat Scholarship