FUDAN 2011 Collaboration of E.coli FUDAN 2011 Unsolved Problems - - PowerPoint PPT Presentation

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FUDAN 2011 Collaboration of E.coli FUDAN 2011 Unsolved Problems - - PowerPoint PPT Presentation

Mar 17, 2023 27 likes •363 views

FUDAN 2011 Collaboration of E.coli FUDAN 2011 Unsolved Problems Our Solution The Project Human practice Achievement check-list Unsolved Problems Complicated genetic circuits Difficulty in Accumulation the Detection of harmful of

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SLIDE 1

Collaboration of E.coli

FUDAN 2011

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SLIDE 2

FUDAN 2011

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SLIDE 3

Unsolved Problems Our Solution The Project Human practice Achievement check-list

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SLIDE 4

Complicated genetic circuits Difficulty in the Detection

  • f growing

phases Accumulation

  • f harmful

byproducts

Unsolved Problems

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SLIDE 5

Solutions

  • Divide

complex processes into different cells

  • Create visible

mark for different growing phases

  • Automatic

harmful byproducts remover

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SLIDE 6

E-tree

  • Construct E.trunk and E.leaf
  • E.trunk detects nitrates in the medium
  • E.leaf changes color according to

various signals

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SLIDE 7

E.trunk

Rh1 I Las I rh1 I ter R NO3 ˉ NO3- Promoter GFP las I RFP

E-tree

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SLIDE 8

E.trunk

GFP YELLOW Rh1 R Las R pRh1 pLas NO3- Promoter

E.trunk

Rh1 I Las I rh1 I ter R NO3 ˉ NO3- Promoter GFP las I RFP

E.leaf

P TetR

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SLIDE 9

E-tree: Genetic Circuits

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SLIDE 10

E-tree: Prospective Results

Without nitrates

With nitrates

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SLIDE 11

E-tree: Results

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SLIDE 12

E-tree: Results

200 400 600 800 1000 1200 0mM 2mM 4mM 6mM 8mM 10mM 20mM 40mM

GFP Flourescent

KO3

pSB1C3 pSB2K3

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SLIDE 13

Solution

  • Divide

complex processes into different cells

  • Create visible

mark for different growing phases

  • Automatic

harmful byproducts remover

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SLIDE 14

Luciferase

  • --2010 CAMBRIDGE

Neon Light: Circuits & Parts

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SLIDE 15

Exponential phase stationary phase

Promoter-----2006 MIT

Neon Light: Circuits & Parts

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SLIDE 16

Neon Light:Overview

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SLIDE 17

Neon light: Prospective Result

Exponential phase stationary phase tube

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SLIDE 18
  • PCR
  • Promoter

BBa_J45996

  • Luciferase

e.g. BBa_K325229

  • Digest
  • Ligate

BBa_J45992 TCTAGA TCTAGA Mutated version of BBa_K325210 TCTAGA BBa_Q04401

Neon light: Method

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SLIDE 19

Exponential phase stationary phase tube

Neon Light:Overview

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SLIDE 20

Solutions

  • Divide the

whole process into different cells

  • Create visible

mark for different growing phases

  • Automatic

harmful byproducts remover

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SLIDE 21

Chef Customer

Dinner Service

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SLIDE 22

AHL AI-2 RFP YFP Lux R Lsr R aiiA Constitutive Promoter Constitutive Promoter Promoter suppressed by LsrR&AI-2 complex Promoter induced by LuxR&AHL complex

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SLIDE 23

Nutrients regulator RFP YFP aiiA Constitutive Promoter Constitutive Promoter Promoter suppressed by regulator Promoter induced by nutrients/byproduct Harmful byproduct

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Human Practice

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Human Practice:Pioneer

We introduce iGEM and thynthetic biology to many students in Fudan University

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SLIDE 26

Survey & Discussion

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SLIDE 27

Detailed Survey

Synthetic biology is widely known by students major in biological sciences, but less known by the

  • thers
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Detailed Survey

More worry about Biological weapons among students of Fudan University

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SLIDE 29

Detailed Survey

Teachers’ efforts & Students’ interests

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SLIDE 30

Team Collaboration

Conduct the survey together with Zhejiang University Keep contact with Shanghai Jiao Tong University

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SLIDE 31

Self Improvement

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CHECK LIST

Characterized the operation of

  • ne pathway and collect data

7 parts are sent to the registry Collaborate with ZJU and Shanghai-JiaoTong University Have a lot of fun in summer

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THANK YOU F