FUDAN 2011 Collaboration of E.coli FUDAN 2011 Unsolved Problems - PowerPoint PPT Presentation

FUDAN 2011 Collaboration of E.coli

FUDAN 2011

Unsolved Problems Our Solution The Project Human practice Achievement check-list

Unsolved Problems Complicated genetic circuits Difficulty in Accumulation the Detection of harmful of growing byproducts phases

Solutions • Automatic • Divide • Create visible harmful mark for complex byproducts different processes into remover growing phases different cells

E-tree • Construct E.trunk and E.leaf • E.trunk detects nitrates in the medium • E.leaf changes color according to various signals

NO 3 ˉ E.trunk E-tree ter R GFP rh1 I NO 3 - Promoter Rh1 I las I RFP Las I

NO 3 ˉ E.trunk E.trunk ter R GFP rh1 I NO 3 - NO 3 - Promoter Promoter Rh1 I las I RFP P TetR E.leaf Rh1 R Las I GFP pRh1 YELLOW Las R pLas

E-tree: Genetic Circuits

E-tree: Prospective Results With nitrates Without nitrates

E-tree: Results

E-tree: Results 1200 1000 GFP Flourescent 800 600 400 200 0 0mM 2mM 4mM 6mM 8mM 10mM 20mM 40mM KO 3 pSB1C3 pSB2K3

Solution • Divide • Automatic • Create visible complex harmful mark for processes byproducts different into different remover growing phases cells

Neon Light: Circuits & Parts Luciferase ---2010 CAMBRIDGE

Neon Light: Circuits & Parts Promoter-----2006 MIT Exponential stationary phase phase

Neon Light:Overview

Neon light: Prospective Result Exponential stationary phase phase tube

Neon light: Method  PCR • Promoter BBa_J45992 BBa_Q04401 BBa_J45996 TCTAGA TCTAGA • Luciferase TCTAGA e.g. Mutated version of BBa_K325210 BBa_K325229  Digest  Ligate

Neon Light:Overview Exponential stationary phase phase tube

Solutions • Automatic • Divide the • Create visible harmful mark for whole process byproducts different into different remover growing phases cells

Dinner Service Customer Chef

aiiA Constitutive Promoter RFP Constitutive Promoter AHL Promoter Lsr R suppressed by LsrR&AI-2 complex Promoter Lux R induced by LuxR&AHL YFP AI-2 complex

aiiA Constitutive Promoter RFP Constitutive Promoter Harmful Nutrients Promoter byproduct suppressed by regulator Promoter induced by YFP nutrients/byproduct regulator

Human Practice

Human Practice:Pioneer We introduce iGEM and thynthetic biology to many students in Fudan University

Survey & Discussion

Detailed Survey Synthetic biology is widely known by students major in biological sciences, but less known by the others

Detailed Survey More worry about Biological weapons among students of Fudan University

Detailed Survey Teachers’ efforts & Students’ interests

Team Collaboration Conduct the survey together with Zhejiang University Keep contact with Shanghai Jiao Tong University

Self Improvement

CHECK LIST Characterized the operation of one pathway and collect data 7 parts are sent to the registry Collaborate with ZJU and Shanghai-JiaoTong University Have a lot of fun in summer

THANK YOU F