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Uncertainty quantification for dynamic spatial proteomic experiments
European Bioconductor conference 2019
Healthy Control AP-4 Deficiency Mis-localisation is phenotypic in AP-4 deficiency syndrome A.K. Davies et al. Nat. Comms. 2018
dynamic spatial proteomic experiments European Bioconductor - - PowerPoint PPT Presentation
dynamic spatial proteomic experiments European Bioconductor - - PowerPoint PPT Presentation
Uncertainty quantification for dynamic spatial proteomic experiments European Bioconductor conference 2019 Mis-localisation is phenotypic in AP-4 deficiency syndrome Healthy Control AP-4 Deficiency A.K. Davies et al. Nat. Comms . 2018
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High-throughput methods for subcellular localisation
Christoforou et al. Nat Comms. 2016, Mulvey et al. Nat Prot. 2017, Thul et al. Science, 2017
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Dynamic spatial proteomics workflow Normal cell population Perturbed cell population Parallel mass-spectrometry workflow
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What proteins re-localise upon perturbation of the subcellular environment? Differential Localisation
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Chi-Squared test Pearson Correlation
Itzhak et al. Elife, Cell reports 2016/17
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Non-parametric model
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Joint allocation for protein i across datasets Conjugate prior Allows us to calculate the probability that a protein is differentially localisation
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