Detecting Tuberculosis SCSU iGEM 2016 What is Synthetic Biology? - - PowerPoint PPT Presentation

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Detecting Tuberculosis SCSU iGEM 2016 What is Synthetic Biology? - - PowerPoint PPT Presentation

Sep 24, 2023 165 likes •454 views

Detecting Tuberculosis SCSU iGEM 2016 What is Synthetic Biology? -Branch of biology that encompasses biotechnology, math, engineering, physics, chemistry, and computer science -Creating genetic circuits / biological components and systems

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Detecting Tuberculosis

SCSU iGEM 2016

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  • Branch of biology that encompasses

biotechnology, math, engineering, physics, chemistry, and computer science

  • Creating genetic circuits / biological

components and systems

What is Synthetic Biology?

ISBN: 978-1-491-90429-9

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What is iGEM?

  • The international genetically

engineered machine competition

  • Teams from around the world

present their projects at the “Giant Jamboree” in October in Boston

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iGEM Parts Registry

  • Catalog of standardized parts
  • All produced by iGEM teams
  • Available for iGEM labs/teams
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Tuberculosis (TB)

*all data taken from the CDC website

  • ⅓ of the world population is

infected with TB

  • In 2014 9.6 million people were

sick with TB

  • There were 1.5 million TB related

deaths in 2014

http://museumofhealthcare.ca/explore/ exhibits/breath/anti-tuberculosis.html

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Tuberculosis Fatality Across the Globe

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Tuberculosis Fatality Across the Globe

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Tuberculosis Detection Methods

doi: 10.1136/postgradmedj-2015-133278

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Our Goal

  • Breath Test
  • Fast Results
  • Distributable
  • Easy to read

http://www.safewaybreathalyzer.com/

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Tuberculosis

Project Overview

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VOCs Tuberculosis

Project Overview

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1-Methylnaphthalene Tuberculosis

Project Overview

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1-Methylnaphthalene Tuberculosis

Project Overview

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1-Methylnaphthalene Tuberculosis Indigo Dye

Project Overview

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3-Methylsalicylate 1-Methylnaphthalene

Indigo Dye

Engineering E. coli

http://pigeoncad.org/

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3-Methylsalicylate 1-Methylnaphthalene

Indigo Dye

Engineering E. coli

http://pigeoncad.org/

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NahR Transcription Factor

http://2013.igem.org/Team:Peking/Project/BioSensors/NahR

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NahR Transcription Factor

http://2013.igem.org/Team:Peking/Project/BioSensors/NahR

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NahR Transcription Factor

http://2013.igem.org/Team:Peking/Project/BioSensors/NahR

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3-Methylsalicylate 1-Methylnaphthalene

Indigo Dye

Engineering E. coli

http://pigeoncad.org/

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Nah Operon

http://www.genome.jp/kegg-bin/show_pathway?map=map00626&show_description=show

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Enzymes

NahA NahB NahC NahD NahE NahF

Genes

Nah Operon

http://www.genome.jp/kegg-bin/show_pathway?map=map00626&show_description=show

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NahR Transcription Factor

http://2013.igem.org/Team:Peking/Project/BioSensors/NahR

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NahR Transcription Factor

http://2013.igem.org/Team:Peking/Project/BioSensors/NahR

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3-Methylsalicylate 1-Methylnaphthalene

Indigo Dye

Engineering E. coli

http://pigeoncad.org/

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Our circuit produces Nah Operon

Indigo Production

http://www.ncbi.nlm.nih.gov/pubmed/6353574

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Enzymes

NahA NahB NahC NahD NahE NahF

Genes

naphthalene 1,2-dioxygenase

Nah Operon

http://www.genome.jp/kegg-bin/show_pathway?map=map00626&show_description=show