cse182 l14
play

CSE182-L14 Population Genetics: Basics Population Structure 377 - PowerPoint PPT Presentation

CSE182-L14 Population Genetics: Basics Population Structure 377 locations (loci) were sampled in 1000 people from 52 populations. 6 genetic clusters were obtained, which corresponded to 5 geographic regions (Rosenberg et al. Science


  1. CSE182-L14 Population Genetics: Basics

  2. Population Structure • 377 locations (loci) were sampled in 1000 people from 52 populations. • 6 genetic clusters were obtained, which corresponded to 5 geographic regions (Rosenberg et al. Science 2003) Oceania Eurasia East Asia America Africa

  3. Population Genetics • What is it about our genetic makeup that makes us measurably different? • These genetic differences are correlated with phenotypic differences • With cost reduction in sequencing and genotyping technologies, we will know the sequence for entire populations of individuals. • Here, we will study the basics of this polymorphism data, and tools that are being developed to analyze it.

  4. What causes variation in a population? • Mutations (may lead to SNPs) • Recombinations • Other genetic events (may lead to microsatellite repeats)

  5. Single Nucleotide Polymorphisms Infinite Sites Assumption: Each site mutates at most once 00000101011 10001101001 01000101010 01000000011 00011110000 00101100110

  6. Short Tandem Repeats GCTAGATCATCATCATCATTGCTAG GCTAGATCATCATCATTGCTAGTTA GCTAGATCATCATCATCATCATTGC GCTAGATCATCATCATTGCTAGTTA GCTAGATCATCATCATTGCTAGTTA GCTAGATCATCATCATCATCATTGC 4 3 5 3 3 5

  7. STR can be used as a DNA fingerprint • Consider a collection of regions with variable length 4 2 3 3 repeats. individuals 5 1 • Variable length repeats will 3 2 lead to variable length DNA 3 1 5 3 • Vector of lengths is a finger- print positions

  8. Recombination 00000000 11111111 00011111

  9. What if there were no recombinations? • Life would be simpler • Each seqence would have a single parent • The relationship is expressed as a tree.

  10. The Infinite Sites Assumption 0 0 0 0 0 0 0 0 3 0 0 1 0 0 0 0 0 5 8 0 0 1 0 1 0 0 0 0 0 1 0 0 0 0 1 The different sites are linked. A 1 in position 8 implies 0 in • position 5, and vice versa. Some phenotypes could be linked to the polymorphisms • Some of the linkage is “destroyed” by recombination •

  11. Infinite sites assumption and Perfect Phylogeny • Each site is mutated at most once in the history. i • All descendants must carry the mutated value, and all others must carry the ancestral value 1 in position i 0 in position i

  12. Perfect Phylogeny • Assume an evolutionary model in which no recombination takes place, only mutation. • The evolutionary history is explained by a tree in which every mutation is on an edge of the tree. All the species in one sub-tree contain a 0, and all species in the other contain a 1. Such a tree is called a perfect phylogeny . • How can one reconstruct such a tree?

  13. The 4-gamete condition • A column i partitions the i set of species into two sets A 0 i 0 , and i 1 i 0 B 0 • A column is homogeneous C 0 D 1 w.r.t a set of species, if it E 1 i 1 has the same value for all F 1 species. Otherwise, it is heterogenous. • EX: i is heterogenous w.r.t {A,D,E}

  14. 4 Gamete Condition • 4 Gamete Condition – There exists a perfect phylogeny if and only if for all pair of columns (i,j), either j is not heterogenous w.r.t i 0 , or i 1 . – Equivalent to – There exists a perfect phylogeny if and only if for all pairs of columns (i,j), the following 4 rows do not exist (0,0), (0,1), (1,0), (1,1)

  15. 4-gamete condition: proof • Depending on which edge the mutation j occurs, either i 0 , or i 1 should be homogenous. i • (only if) Every perfect phylogeny satisfies the 4-gamete condition • (if) If the 4-gamete condition is satisfied, does a prefect i 0 i 1 phylogeny exist?

  16. An algorithm for constructing a perfect phylogeny • We will consider the case where 0 is the ancestral state, and 1 is the mutated state. This will be fixed later. • In any tree, each node (except the root) has a single parent. – It is sufficient to construct a parent for every node. • In each step, we add a column and refine some of the nodes containing multiple children. • Stop if all columns have been considered.

  17. Inclusion Property • For any pair of columns i,j – i < j if and only if i 1 ⊇ j 1 • Note that if i<j then the edge containing i is an i ancestor of the edge containing i j

  18. Example r 1 2 3 4 5 A 1 1 0 0 0 B 0 0 1 0 0 C 1 1 0 1 0 A B C D E D 0 0 1 0 1 E 1 0 0 0 0 Initially, there is a single clade r, and each node has r as its parent

  19. Sort columns • Sort columns according to the inclusion property (note that the columns are already sorted here). • This can be achieved by considering the columns as 1 2 3 4 5 binary representations of A 1 1 0 0 0 numbers (most significant bit B 0 0 1 0 0 in row 1) and sorting in C 1 1 0 1 0 decreasing order D 0 0 1 0 1 E 1 0 0 0 0

  20. Add first column 1 2 3 4 5 A 1 1 0 0 0 • In adding column i B 0 0 1 0 0 C 1 1 0 1 0 – Check each edge and D 0 0 1 0 1 E 1 0 0 0 0 decide which side you r belong. – Finally add a node if u you can resolve a clade B D A C E

  21. Adding other columns 1 2 3 4 5 A 1 1 0 0 0 • Add other B 0 0 1 0 0 C 1 1 0 1 0 columns on D 0 0 1 0 1 E 1 0 0 0 0 edges using the r ordering 1 3 property E 2 B 5 4 D A C

  22. Unrooted case • Switch the values in each column, so that 0 is the majority element. • Apply the algorithm for the rooted case

  23. Handling recombination • A tree is not sufficient as a sequence may have 2 parents • Recombination leads to loss of correlation between columns

  24. Linkage (Dis)-equilibrium (LD) • Consider sites A &B A B • Case 1: No recombination 0 1 – Pr[A,B=0,1] = 0.25 0 1 0 0 • Linkage disequilibrium 0 0 1 0 • Case 2:Extensive 1 0 recombination 1 0 – Pr[A,B=(0,1)=0.125 1 0 • Linkage equilibrium

Recommend


More recommend