Cross-Kingdom Similarity of Transcriptional Regulation in Innate - PowerPoint PPT Presentation

Platzhalter für Bild, Bild auf Titelfolie hinter das Logo einsetzen Cross-Kingdom Similarity of Transcriptional Regulation in Innate Immunity Reinhard Hehl, Konstantin Kanofsky, Annika Möller, Alexander Sandmann Institute of Genetics, TU Braunschweig

From the Greening of the TRANSFAC Database............

............to a Rich Harvest

............ and to our own Databases like AthaMap Steffens et al., (2004) Nucleic Acids Res. 32: D368-372. Hehl et al., (2016) Plant Cell Physiol 57: e4

............and PathoPlant Bülow et al., (2004) In Silico Biol. 4: 529–536 Bolívar et al., (2014) Database (Oxford) 2014: bau030

Identification of Novel Cis -Regulatory Sequences Involved in Plant Innate Immunity Bent and Mackey (2007) Annu Rev Phytopathol 45: 399-436

Novel Cis -Regulatory Sequences for Synthetic Pathogen-Responsive Promoters Lehmeyer et al. (2016) Plant Biotechnology Journal 14: 61-71

Application of Synthetic Pathogen-Responsive Promoters Bent and Mackey (2007) Annu Rev Phytopathol 45: 399-436

Application of Synthetic Pathogen-Responsive Promoters Dietmar Stahl, KWS SAAT AG, modified

Application of Synthetic Pathogen-Responsive Promoters (Helicase of TMV) Agrobacterium tumefaciens (N) Dietmar Stahl, KWS SAAT AG, modified

Application of Synthetic Pathogen-Responsive Promoters Niemeyer et al., (2014) Plant Mol Biol 84: 111-123

Identification of Novel Cis -Regulatory Sequences Involved in Plant Innate Immunity Hehl and Bülow (2008) Current Genomics 9: 375-380

Identification of Novel Cis -Regulatory Sequences Involved in Plant Innate Immunity Koschmann et al., (2012) Plant Physiol 160: 178-191

Identification of Novel Cis -Regulatory Sequences Involved in Plant Innate Immunity 3. Step Motif classification using STAMP 37 motif groups identified Koschmann et al., (2012) Plant Physiol 160: 178-191

Identification of Novel Cis -Regulatory Sequences Involved in Plant Innate Immunity Spe I Xba I 4 x cis -sequences TATA uid A Koschmann et al., (2012) Plant Physiol 160: 178-191

Identification of Novel Cis -Regulatory Sequences Involved in Plant Innate Immunity Koschmann et al., (2012) Plant Physiol 160: 178-191

Identification of Novel Cis -Regulatory Sequences Involved in Plant Innate Immunity WT-box Type Binding TF CGACTTTT I WRKY70 TGACTTTT I WRKY70 GGACTTTT II ? GGACTTTG II ? GGACTTTC II ? Kanofsky et al., (2017) Plant Cell Rep. 36: 971-986 Lehmeyer et al., (2016) Plant Biotechnol J 14: 61–71 Machens et al., (2014) Plant Mol Biol 84: 371-385

Identification of Novel Cis -Regulatory Sequences Involved in Plant Innate Immunity WRKY70 CRM1 WRKY26 mut2 mut5 W-box WT-box W-box Kanofsky et al., (2017) Plant Cell Rep. 36: 971-986

Identification of Novel Cis -Regulatory Sequences Involved in Plant Innate Immunity WRKY26 W-box WT-box W-box Kanofsky et al., (2017) Plant Cell Rep. 36: 971-986

Identification of Novel Cis -Regulatory Sequences Involved in Plant Innate Immunity WT-box Type Binding TF CGACTTTT I WRKY70 TGACTTTT I WRKY70 GGACTTTT II ? GGACTTTG II ? GGACTTTC II WRKY26 Kanofsky et al., (2017) Plant Cell Rep. 36: 971-986

Bioinformatic Prediction of Type II WT-box Interacting Proteins FootprintDB Contreras-Moreira (2010) Nucleic Acids Res 38: D91-97 Sebastian and Contreras-Moreira (2014) Bioinformatics 30: 258-265

Bioinformatic Prediction of Type II WT-box Interacting Proteins Kanofsky et al., unpublished

Bioinformatic Prediction of Type II WT-box Interacting Proteins Ausubel (2005) Nat Immunol 6: 973-979, modified

Bioinformatic Prediction of Type II WT-box Interacting Proteins 140000 ¡ 120000 ¡ pmol ¡4-­‑MU/min/mg ¡ 100000 ¡ 80000 ¡ 60000 ¡ 40000 ¡ 20000 ¡ 0 ¡ S15 ¡ S15mut1 ¡ S15mut2 ¡ S15mut3 ¡ pORE ¡ p65-­‑pORE ¡ S15 AGGACTTTTCACCAGTTGGACTTTGAAGCCACCAA S15mut1 --AGTCCCC-------------------------- S15mut2 ---------TCTTCACCA----------------- S15mut3 ------------------AGTCCCA---------- Kanofsky et al., unpublished

Bioinformatic Prediction of Type II WT-box Interacting Proteins 120.000 ¡ 100.000 ¡ pmol ¡4-­‑MU/min/mg ¡ 80.000 ¡ 60.000 ¡ 40.000 ¡ 20.000 ¡ 0 ¡ S24 ¡ S24mut8 ¡ S24mut5 ¡ S24mut9 ¡ S24mut4 ¡ pORE ¡ p65-­‑pORE ¡ S24 TGGTCAGCATGTTGGACTTTCCAAATTCATTGACC S24mut8 ------ATGCACC---------------------- S24mut5 -------------AAGTCCCTT------------- S24mut9 ----------------------GGGCCTG------ S24mut4 -----------------------------CCAGTT Kanofsky et al., unpublished

Bioinformatic Prediction of Type II WT-box Interacting Proteins Kanofsky et al., unpublished

Identification of Novel Cis -Regulatory Sequences Involved in Plant Innate Immunity WT-box Type Binding TF CGACTTTT I WRKY70 TGACTTTT I WRKY70 GGACTTTT II NF- ! B p65 GGACTTTG II ? GGACTTTC II WRKY26 NF- ! B p65 Kanofsky et al., (2017) Plant Cell Rep. 36: 971-986 Kanofsky et al., unpublished

Bioinformatic Prediction of Type II WT-box Interacting Proteins ? Ausubel (2005) Nat Immunol 6: 973-979, modified

Edgar‘s Post-Retirement Endeavour in Swakopmund, Namibia Edgar‘s

Thank You Spe I Xba I 4 x cis -sequences TATA uid A