Cross-Kingdom Similarity of Transcriptional Regulation in Innate - - PowerPoint PPT Presentation

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Cross-Kingdom Similarity of Transcriptional Regulation in Innate - - PowerPoint PPT Presentation

Oct 15, 2023 269 likes •582 views

Platzhalter fr Bild, Bild auf Titelfolie hinter das Logo einsetzen Cross-Kingdom Similarity of Transcriptional Regulation in Innate Immunity Reinhard Hehl, Konstantin Kanofsky, Annika Mller, Alexander Sandmann Institute of Genetics, TU

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Platzhalter für Bild, Bild auf Titelfolie hinter das Logo einsetzen

Reinhard Hehl, Konstantin Kanofsky, Annika Möller, Alexander Sandmann Institute of Genetics, TU Braunschweig

Cross-Kingdom Similarity of Transcriptional Regulation in Innate Immunity

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From the Greening of the TRANSFAC Database............

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............to a Rich Harvest

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............ and to our own Databases like AthaMap

Steffens et al., (2004) Nucleic Acids Res. 32: D368-372. Hehl et al., (2016) Plant Cell Physiol 57: e4

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............and PathoPlant

Bülow et al., (2004) In Silico Biol. 4: 529–536 Bolívar et al., (2014) Database (Oxford) 2014: bau030

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Identification of Novel Cis-Regulatory Sequences Involved in Plant Innate Immunity

Bent and Mackey (2007) Annu Rev Phytopathol 45: 399-436

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Novel Cis-Regulatory Sequences for Synthetic Pathogen-Responsive Promoters

Lehmeyer et al. (2016) Plant Biotechnology Journal 14: 61-71

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Application of Synthetic Pathogen-Responsive Promoters

Bent and Mackey (2007) Annu Rev Phytopathol 45: 399-436

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Dietmar Stahl, KWS SAAT AG, modified

Application of Synthetic Pathogen-Responsive Promoters

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Dietmar Stahl, KWS SAAT AG, modified

Application of Synthetic Pathogen-Responsive Promoters

Agrobacterium tumefaciens (Helicase of TMV) (N)

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Application of Synthetic Pathogen-Responsive Promoters

Niemeyer et al., (2014) Plant Mol Biol 84: 111-123

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Hehl and Bülow (2008) Current Genomics 9: 375-380

Identification of Novel Cis-Regulatory Sequences Involved in Plant Innate Immunity

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Identification of Novel Cis-Regulatory Sequences Involved in Plant Innate Immunity

Koschmann et al., (2012) Plant Physiol 160: 178-191

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Identification of Novel Cis-Regulatory Sequences Involved in Plant Innate Immunity

Koschmann et al., (2012) Plant Physiol 160: 178-191

Motif classification using STAMP 37 motif groups identified

  • 3. Step
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SpeI XbaI 4 x cis-sequences TATA uidA

Identification of Novel Cis-Regulatory Sequences Involved in Plant Innate Immunity

Koschmann et al., (2012) Plant Physiol 160: 178-191

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Identification of Novel Cis-Regulatory Sequences Involved in Plant Innate Immunity

Koschmann et al., (2012) Plant Physiol 160: 178-191

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Identification of Novel Cis-Regulatory Sequences Involved in Plant Innate Immunity

WT-box Type Binding TF CGACTTTT I WRKY70 TGACTTTT I WRKY70 GGACTTTT II ? GGACTTTG II ? GGACTTTC II ?

Kanofsky et al., (2017) Plant Cell Rep. 36: 971-986 Lehmeyer et al.,(2016) Plant Biotechnol J 14: 61–71 Machens et al., (2014) Plant Mol Biol 84: 371-385

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Identification of Novel Cis-Regulatory Sequences Involved in Plant Innate Immunity

CRM1 mut2 mut5

Kanofsky et al., (2017) Plant Cell Rep. 36: 971-986

WRKY70 WRKY26

WT-box W-box W-box

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Identification of Novel Cis-Regulatory Sequences Involved in Plant Innate Immunity

Kanofsky et al., (2017) Plant Cell Rep. 36: 971-986

WRKY26

WT-box W-box W-box

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Identification of Novel Cis-Regulatory Sequences Involved in Plant Innate Immunity

WT-box Type Binding TF CGACTTTT I WRKY70 TGACTTTT I WRKY70 GGACTTTT II ? GGACTTTG II ? GGACTTTC II WRKY26

Kanofsky et al., (2017) Plant Cell Rep. 36: 971-986

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Bioinformatic Prediction of Type II WT-box Interacting Proteins FootprintDB

Contreras-Moreira (2010) Nucleic Acids Res 38: D91-97 Sebastian and Contreras-Moreira (2014) Bioinformatics 30: 258-265

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Bioinformatic Prediction of Type II WT-box Interacting Proteins

Kanofsky et al., unpublished

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Bioinformatic Prediction of Type II WT-box Interacting Proteins

Ausubel (2005) Nat Immunol 6: 973-979, modified

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Bioinformatic Prediction of Type II WT-box Interacting Proteins

Kanofsky et al., unpublished

0 ¡ 20000 ¡ 40000 ¡ 60000 ¡ 80000 ¡ 100000 ¡ 120000 ¡ 140000 ¡ S15 ¡ S15mut1 ¡ S15mut2 ¡ S15mut3 ¡ pmol ¡4-­‑MU/min/mg ¡

pORE ¡ p65-­‑pORE ¡

S15 AGGACTTTTCACCAGTTGGACTTTGAAGCCACCAA S15mut1 --AGTCCCC-------------------------- S15mut2 ---------TCTTCACCA----------------- S15mut3 ------------------AGTCCCA----------

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Bioinformatic Prediction of Type II WT-box Interacting Proteins

Kanofsky et al., unpublished

0 ¡ 20.000 ¡ 40.000 ¡ 60.000 ¡ 80.000 ¡ 100.000 ¡ 120.000 ¡ S24 ¡ S24mut8 ¡ S24mut5 ¡ S24mut9 ¡ S24mut4 ¡ pmol ¡4-­‑MU/min/mg ¡

pORE ¡ p65-­‑pORE ¡

S24 TGGTCAGCATGTTGGACTTTCCAAATTCATTGACC S24mut8 ------ATGCACC---------------------- S24mut5 -------------AAGTCCCTT------------- S24mut9 ----------------------GGGCCTG------ S24mut4 -----------------------------CCAGTT

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Bioinformatic Prediction of Type II WT-box Interacting Proteins

Kanofsky et al., unpublished

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Identification of Novel Cis-Regulatory Sequences Involved in Plant Innate Immunity

WT-box Type Binding TF CGACTTTT I WRKY70 TGACTTTT I WRKY70 GGACTTTT II NF-!B p65 GGACTTTG II ? GGACTTTC II WRKY26 NF-!B p65

Kanofsky et al., (2017) Plant Cell Rep. 36: 971-986 Kanofsky et al., unpublished

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Bioinformatic Prediction of Type II WT-box Interacting Proteins

Ausubel (2005) Nat Immunol 6: 973-979, modified

?

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Edgar‘s Post-Retirement Endeavour in Swakopmund, Namibia

Edgar‘s

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Thank You

SpeI XbaI 4 x cis-sequences TATA uidA