Chlo FOURQUIN Laboratoire RDP-ENS Lyon-France Incoming Agreenskills - PowerPoint PPT Presentation

Chloé FOURQUIN Laboratoire RDP-ENS Lyon-France Incoming Agreenskills fellowship from Spain April 2014-March 2016 Seed Development

Background

Arabidopsis seed development WT zou Seed coat zou Endosperm Embryo Pre-globular Globular Heart Torpedo Mature From Xing et al., 2013 and Denay et al., 2014 http://seedgenenetwork.net/

Characterization of ZOU function in endosperm breakdown Transcriptional analyses WT/ zou Cell wall modification/ cell softening ZOU Identification of high- confidence targets of ZOU Endosperm involved in cell wall breakdown modification and degradation

Multidisciplinary approach 1. Biochemical properties of zou /WT endosperm 2. Mechanical properties of zou /WT endosperm 3. Functional studies of ZOU targets 30 μm indentation 100 μm Hysitron nano 4. Comparative analysis in Maize indenteur 200- 250 μm width

4. Comparative analysis in Maize Endosperm Embryo

Thanks Biophysics team Seeddev team Léna Beauzamy Gwyneth Ingram Audrey Creff Arezki Boudaoud Steven Moussu Andrea Watt Peter Rogowsky Nathalie Depège Thomas Widiez Aurélie Grimault Ghislaine Gendrot