Chlo FOURQUIN Laboratoire RDP-ENS Lyon-France Incoming Agreenskills - - PowerPoint PPT Presentation

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Sep 18, 2023 243 likes •325 views

Chlo FOURQUIN Laboratoire RDP-ENS Lyon-France Incoming Agreenskills fellowship from Spain April 2014-March 2016 Seed Development Background Arabidopsis seed development WT zou Seed coat zou Endosperm Embryo Pre-globular Globular Heart

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Seed Development Chloé FOURQUIN Laboratoire RDP-ENS Lyon-France Incoming Agreenskills fellowship from Spain April 2014-March 2016

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Background

SLIDE 3

zou WT

Arabidopsis seed development

From Xing et al., 2013 and Denay et al., 2014 http://seedgenenetwork.net/

zou

Pre-globular Globular Heart Torpedo Mature Seed coat Embryo Endosperm

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Characterization of ZOU function in endosperm breakdown

ZOU Cell wall modification/ cell softening Endosperm breakdown

Transcriptional analyses WT/zou Identification of high- confidence targets of ZOU involved in cell wall modification and degradation

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1. Biochemical properties of zou/WT endosperm
2. Mechanical properties of zou/WT endosperm

30 μm indentation 200-250 μm width 100 μm Hysitron nano indenteur

3. Functional studies of ZOU targets

Multidisciplinary approach

4. Comparative analysis in Maize

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4. Comparative analysis in Maize

Embryo Endosperm

SLIDE 7

Thanks

Seeddev team Gwyneth Ingram Audrey Creff Steven Moussu Andrea Watt Peter Rogowsky Nathalie Depège Thomas Widiez Aurélie Grimault Ghislaine Gendrot Biophysics team Léna Beauzamy Arezki Boudaoud