By Theo Busschau MSc Supervisor: Prof. Savel Daniels - - PowerPoint PPT Presentation

by theo busschau msc supervisor prof savel daniels co
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By Theo Busschau MSc Supervisor: Prof. Savel Daniels - - PowerPoint PPT Presentation

By Theo Busschau MSc Supervisor: Prof. Savel Daniels Co-supervisor: Werner Conradie Introduction Eastern Cape Forest Project funded by the FBIP The effect of habitat fragmentation on faunal diversity of Eastern Cape forests South


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By Theo Busschau MSc

Supervisor: Prof. Savel Daniels Co-supervisor: Werner Conradie

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Introduction

 Eastern Cape Forest Project funded by the FBIP The effect of habitat fragmentation on faunal diversity of Eastern Cape forests  South African forests are largely fragmented with high diversity  >420 described reptile species in South Africa but forest reptile diversity is

relatively low

 Few studies on forest reptiles in South Africa

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Forest Thread Snake Leptotyphlops sylvicolus Pondo Flat-gecko Afroedura pondolia

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Aims and Objectives

 Are there cryptic lineages within the study taxa?  How are these lineages geographically distributed?  What is the degree of phylogeographic congruence between

these taxa?

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Materials and Methods

Genes Molecular analyses

  • mtDNA:
  • Cytb
  • ND4
  • nuDNA:
  • PRLR
  • RAG1
  • Maximum likelihood – Garli
  • Bayesian inference – MrBayes
  • Divergence time estimation –

BEAUti + BEAST

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Leptotyphlops sylvicolus

88 Samples 21 Localities

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Afroedura pondolia 99 Samples 23 Localities

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Way forward…

  • Haplotype networks
  • AMOVA
  • Clustering analysis on the nuDNA
  • Species delimitation
  • PCA on morphological data
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Acknowledgments

  • NRF-FBIP
  • Bursary and funding
  • Port Elizabeth Museum – Bayworld
  • Hosting and ethical clearance
  • KZN Wildlife and Eastern Cape Parks and T
  • urism
  • Permits