Biological Engineering Education and Computational Methods Effect - - PowerPoint PPT Presentation

Biological Engineering Education and Computational Methods

Effect of the Geometrical Structure

• f Mitochondria on the Synthesis and

Diffusion of Oxidative Metabolites

Rajan Gangadharan1 and Dr.Guigen Zhang1,2,3

1Micro/Nano Bioengineering Laboratory, 2Nanoscale Science and Engineering

Center, 3Faculty of Engineering, The University of Georgia, Athens, GA 30602

Introduction

• Abundant new information on the structure

and dynamics of living cells are currently available

– Aided by advances in Microscopy & Molecular biology.

• Computational approaches are needed to

link all these new data

• Issues in computational approach: Cell

systems are complex

– Interdependent chemical reactions and structural components regulates cell processes

Introduction

• Spatiotemporal computational approaches ‐
• ne way to answer the complexity
• Partial differential equations ‐ defines

concentration changes in space and time.

– ODEs can define reactions, membrane transport and electrical potential – Diffusion of molecules within complex geometry defined by using PDEs

Research background – Mitochondria cristae structure

Research Questions

Previous Work

Model Development

Model Development

Model Geometry

Model Development

Model Development

Example: For complex I the reaction rate is defined as

Results – Effect of the tubular junction on ADP concentration

Results – Effect on membrane potential

Effect of diameter of tubular connection on potential Time (s)

10-12 10-11 10-1010-9 10-8 10-7 10-6 10-5 10-4 10-3 10-2 10-1 100 101 102 103 104

Membrane Potential (mV)

150 160 170 180 190 200 No tubular connection Tubular connection - 40nm dia Tubular connection - 20 nm dia

Potential change at various points on the inner membrane and cristae Time (s)

10-12 10-11 10-1010-9 10-8 10-7 10-6 10-5 10-4 10-3 10-2 10-1 100 101 102 103 104

Membrane Potential (mV)

155 160 165 170 175 180 185 190 195 Point A Point B Point C Point D

Conclusion and Further Work

I thank Dr. Guigen Zhang, My group members and Faculty

• f Engineering ,

University of Georgia for their support

Thank You