BI OREACTORS NEW SOLUTI ONS FOR OLD PROBLEMS Dr. Pavel Lehky - - PowerPoint PPT Presentation

▶
bi oreactors new soluti ons for old problems
SMART_READER_LITE
LIVE PREVIEW

BI OREACTORS NEW SOLUTI ONS FOR OLD PROBLEMS Dr. Pavel Lehky - - PowerPoint PPT Presentation

Aug 01, 2023 162 likes •508 views

BI OREACTORS NEW SOLUTI ONS FOR OLD PROBLEMS Dr. Pavel Lehky LAMBDA Laborat ory I nst rument s www.rhone.ch/ lambda 1 st I nternational Congress on Bioreactor Technology www.rhone.ch/ lambda OUTLI NE St irring mechanism I R-heat

slide-1
SLIDE 1
  • Dr. Pavel Lehky

LAMBDA – Laborat ory I nst rument s

www.rhone.ch/ lambda

BI OREACTORS NEW SOLUTI ONS FOR OLD PROBLEMS

www.rhone.ch/ lambda

1st I nternational Congress on Bioreactor Technology

slide-2
SLIDE 2

OUTLI NE

www.rhone.ch/ lambda

1st I nternational Congress on Bioreactor Technology

  • St irring mechanism
  • I R-heat ing syst em
  • Approach used in t he MI NI FOR
  • Probes
  • Ferment at ion accessories (Pumps and gas

f low cont roller)

  • Conclusions
slide-3
SLIDE 3

TRADI TI ONAL STI RRI NG

a) Mechanical seals

  • No physical separat ion
  • Cont aminat ions
  • Service “sensit ive”

www.rhone.ch/ lambda

1st I nternational Congress on Bioreactor Technology

slide-4
SLIDE 4

TRADI TI ONAL STI RRI NG

b) Magnetic coupling

  • Separat ion St erilit y
  • Dead spaces
  • High cost

www.rhone.ch/ lambda

1st I nternational Congress on Bioreactor Technology

slide-5
SLIDE 5

NEW STI RRI NG SCHEME

Up and down movement

www.rhone.ch/ lambda

1st I nternational Congress on Bioreactor Technology

slide-6
SLIDE 6

NEW STI RRI NG SCHEME

Fermentor head

  • Separat ion St erilit y
  • Elect romagnet Mot or
  • Easy t o mount (one hand)
  • Threaded necks
  • Low cost

www.rhone.ch/ lambda

1st I nternational Congress on Bioreactor Technology

slide-7
SLIDE 7

NEW STI RRI NG SCHEME

www.rhone.ch/ lambda

1st I nternational Congress on Bioreactor Technology

Stirring discs

  • Count er rot at ing f low pat hs
  • Vert ical st ream (t hrough t he

holes)

slide-8
SLIDE 8

NEW STI RRI NG SCHEME

Stirring properties

  • Whole volume st irring
  • Good gas dist ribut ion
  • No vort ex f ormed
  • No baf f les needed
  • Reduced f oaming
  • Gent le t o cells

www.rhone.ch/ lambda

1st I nternational Congress on Bioreactor Technology

slide-9
SLIDE 9

NEW STI RRI NG SCHEME

I n action…

www.rhone.ch/ lambda

1st I nternational Congress on Bioreactor Technology

slide-10
SLIDE 10

a) Capillary

Possible blocking by salt s

AI R SPARGERS

b) Silicone

  • No blocking by salt s
  • Good bot t om circulat ion

and bubble breaking

www.rhone.ch/ lambda

1st I nternational Congress on Bioreactor Technology

slide-11
SLIDE 11

FERMENTATI ON VESSELS

  • Adopt ed f rom cell

cult ure

  • Up t o 8 t hreaded side

necks

  • Good accessibilit y
  • Variabilit y
  • Low cost
  • “j acket able”

www.rhone.ch/ lambda

1st I nternational Congress on Bioreactor Technology

slide-12
SLIDE 12

STOPPER

  • Ef f icient sealing
  • Reusable many t imes

www.rhone.ch/ lambda

1st I nternational Congress on Bioreactor Technology

slide-13
SLIDE 13

“COFFEE CUP” RADI ATOR

  • I R-heat ing gent le sun-like

heat ing

  • Nat ural convect ion
  • No cont act wit h vessel
  • No overheat ing at any volume
  • Up t o 60°C in 3l vessel
  • 98% energy yield
  • Negligible heat capacit y
  • Low volt age

www.rhone.ch/ lambda

1st I nternational Congress on Bioreactor Technology

slide-14
SLIDE 14

THE MI NI FOR APPROACH

Fermentor case

  • I nt egrat ed f ree-st anding

vessel

  • Cascaded design
  • Ergonomic arrangement
  • Excellent accessibilit y
  • Ext remely compact

www.rhone.ch/ lambda

1st I nternational Congress on Bioreactor Technology

slide-15
SLIDE 15

THE MI NI FOR APPROACH

Control panel

  • Closest t o operat or
  • All inf ormat ion at a

glance (no scrolling or menu select ion)

  • Easy operat ion

www.rhone.ch/ lambda

1st I nternational Congress on Bioreactor Technology

slide-16
SLIDE 16

THE MI NI FOR APPROACH

Radiator

  • Sealing vessel holder

spill prot ect ion

  • Vessels above heat

source

  • Shielding of t he hot

coil prot ect ion against burning

www.rhone.ch/ lambda

1st I nternational Congress on Bioreactor Technology

slide-17
SLIDE 17

THE MI NI FOR APPROACH

Front connectors

  • Short est connect ion

bet ween probes and vessel

  • Sealed cables

www.rhone.ch/ lambda

1st I nternational Congress on Bioreactor Technology

slide-18
SLIDE 18

THE MI NI FOR APPROACH

Fermentor platf orm

  • Air out put and st orage

bot t les behind t he vessel

  • Short est connect ion

bet ween pumps and vessel

  • Magnet ic holders

www.rhone.ch/ lambda

1st I nternational Congress on Bioreactor Technology

slide-19
SLIDE 19

THE MI NI FOR APPROACH

Rear side

www.rhone.ch/ lambda

1st I nternational Congress on Bioreactor Technology

slide-20
SLIDE 20

THE MI NI FOR APPROACH

General view

  • Up t o 4 pumps on support s
  • Pump prot ect ion in case of

t ubing rupt ure

  • Good visibilit y of pump

st at us

  • Easy accessibilit y f rom all

sides

www.rhone.ch/ lambda

1st I nternational Congress on Bioreactor Technology

slide-21
SLIDE 21

THE MI NI FOR APPROACH

Serial f ermentation

  • Opt imisat ion in series
  • Can be used separat ely (complet ely independent )

www.rhone.ch/ lambda

1st I nternational Congress on Bioreactor Technology

slide-22
SLIDE 22

PROBES

pH and temperature probe

  • Variopin connect or (f reely st erilisable)
  • Pt 100 int egrat ed in t he glass elect rode

www.rhone.ch/ lambda

1st I nternational Congress on Bioreactor Technology

slide-23
SLIDE 23

PROBES

DO probe

  • PEEK body
  • Freely st erilisable connect or
  • Large cat hode syst em

www.rhone.ch/ lambda

1st I nternational Congress on Bioreactor Technology

slide-24
SLIDE 24

PROBES

DO probe membrane module

  • Glass support ed PTFE

membrane

  • Fast response
  • Membrane prot ect ion

www.rhone.ch/ lambda

1st I nternational Congress on Bioreactor Technology

slide-25
SLIDE 25

I NOCULATI ON & SAMPLI NG

  • St ainless st eel

capillaries

  • Luer-Lock

adapt ers

www.rhone.ch/ lambda

1st I nternational Congress on Bioreactor Technology

slide-26
SLIDE 26

PERI STALTI C PUMPS

www.rhone.ch/ lambda

1st I nternational Congress on Bioreactor Technology

  • Compact
  • Reliable
  • Economy of t ubing
  • Progressive speed cont rol
  • Const ant compression

cont rol

  • Large rollers
  • Asymmet ric head
slide-27
SLIDE 27

PERI STALTI C PUMPS

I n action…

www.rhone.ch/ lambda

1st I nternational Congress on Bioreactor Technology

slide-28
SLIDE 28

MASSFLOW

Gas f low control

  • Cont rolled aut omat ic

addit ion of CO2 or N2 during cell cult ures

  • No need of expensive

gas st at ions

  • Programmable
  • Tot aliser

www.rhone.ch/ lambda

1st I nternational Congress on Bioreactor Technology

slide-29
SLIDE 29

CELL CULTURE ADAPTOR

  • Very gent le st irring
  • Oxygenat ion t hrough moving coil

www.rhone.ch/ lambda

1st I nternational Congress on Bioreactor Technology

slide-30
SLIDE 30

I NTEGRATOR

  • Tot alisat ion of

delivered liquid or gas

  • Access t o growt h

kinet ics

  • Enzyme act ivit y

measurement

www.rhone.ch/ lambda

1st I nternational Congress on Bioreactor Technology

slide-31
SLIDE 31

FERMENTATI ON

www.rhone.ch/ lambda

1st I nternational Congress on Bioreactor Technology

Addit ion of acid or base necessary t o keep t he pH const ant would not appear wit hout PRECI FLOW and I NTEGRATOR !

slide-32
SLIDE 32

FNet – SOFTWARE (Sysmatec)

www.rhone.ch/ lambda

1st I nternational Congress on Bioreactor Technology

  • Dat a saving
  • Graph scaling
  • Prof ile def init ion
  • Alarms
  • Complet e easy t o use sof t ware f or

laborat ory f erment ors

  • Connect ion of up t o 6 f erment ors,

12 int egrat ors and 6 pumps

slide-33
SLIDE 33

CONCLUSI ONS

  • A maj or ef f ort was invest ed t o make

bioreact ors more ef f icient and simple

  • New up and down st irring concept providing

easy st erilit y

  • I R-heat er f or gent le heat ing
  • pH and DO probes
  • Pumps and gas f low cont roller

www.rhone.ch/ lambda

1st I nternational Congress on Bioreactor Technology

slide-34
SLIDE 34

This work would not have been possible without:

Jaroslav Bernášek, Vladimir ermák, Rainer Glöckler, Vlastimil Hanuš, Hans Kulla, Lida Lehká, Jan Marc Lehky, Petr Lehky, Stanislav Tíska, Daniel Venetz, Patrick Yax, Michal Zemek

www.rhone.ch/ lambda

1st I nternational Congress on Bioreactor Technology

THANK YOU !!!