Bacterial Source Tracking April 1, 2016 San Antonio River Basin - - PowerPoint PPT Presentation

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Bacterial Source Tracking April 1, 2016 San Antonio River Basin - - PowerPoint PPT Presentation

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Bacterial Source Tracking April 1, 2016 San Antonio River Basin Basin 19 1905 13 Classified Segments, including 1902 1910 1911 Basin # Segment # 1903 1901 2 San Antonio River Basin Segments divided into Assessment Units

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SLIDE 1

Bacterial Source Tracking

April 1, 2016

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SLIDE 2

San Antonio River Basin

  • Basin 19
  • 13 Classified Segments,

including…

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1901 1902 1903 1905 1910 1911

Basin # Segment #

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SLIDE 3

San Antonio River Basin

  • Segments divided into Assessment Units

(AU’s)

  • Cibolo Creek:

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– 1902_01 – Lower 5 miles of segment – 1902_02 – From 5 mi. upstream of confluence with the SAR to FM 541 – 1902_03 – FM 541 to confluence w/Clifton Branch – 1902_04 – Confluence w/Clifton Branch to confluence w/ Elm Creek – 1902_05 – Upper end of segment

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SLIDE 4

Bacterial Impairments

  • Primary Contact Recreation standard on

the 13 classified segments:

– 126 colony forming units (cfu)/100 mL

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50 colonies in a 10 mL dilution = 500 cfu/100 mL

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SLIDE 5

Bacterial Impairments

  • Segments

w/Impairments: 1901 – Lower SAR* 1902 – Lower Cibolo* 1903 – Lower Medina 1906 – Lower Leon 1908 – Upper Cibolo 1910 – Salado Creek 1911 – Upper SAR*

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  • Segments w/o Impairments:

1904 – Medina Lake 1905 – Upper Medina 1907 – Upper Leon 1909 – Medina Diversion Lake 1912 – Medio Creek 1913 – Mid-Cibolo*

*Denotes segments w/BST sample sites

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SLIDE 6

Bacterial Impairments

  • 83 Assessment Units (Including

Unclassified Segments)

  • 29 (34.9%) w/bacterial impairments
  • Kendall, Bexar, Wilson, Karnes & Goliad

Counties

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SLIDE 7

Why Bacterial Source Tracking (BST)?

  • Working Hypothesis…

– Agricultural impact in urban waterways – Human Impact in rural waterways

  • Shift in thought

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Lower Cibolo Creek

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SLIDE 8

Why BST?

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Lower Cibolo Creek

To try and better understand the sources of bacterial loading contributing to the impairments throughout the San Antonio River basin.

Lower Cibolo Creek

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SLIDE 9

What is BST?

  • Fundamental idea is that some intestinal

bacteria differs from one animal group to another, due to:

– Basic Habitat

  • Body temp., food supply, digestive system, etc.

– Natural Selection

  • Direct competition, prior exposure to agents like

antibiotics

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SLIDE 10

What is BST?

  • Library “Independent” vs. “Dependent”:

– Both depend on ‘sourcing’ bacteria from known organisms – Both use ‘signatures’ to identify sources of bacterial contamination – Dependent methods require a ‘library’ to be constantly updated

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SLIDE 11

Methods

  • Field Observations

– Weather, recreation, flow level, water color, signs of wildlife

  • Field Parameters:

– Flow, DO, pH, specific conductance, temperature, days since last precipitation event

  • Composite Sample

– Grabs 5-minutes apart

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SLIDE 12

BST Methods

  • Library-dependent analysis,

sources identified by AgriLife using ERIC-PCR and RiboPrinting

  • 10 Isolates/sample
  • Results in 3-Way & 7-Way Splits

– High confidence – Less confidence in species classifications

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SLIDE 13

13

  • Phase 1
  • Phase 2
  • Phase 3
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SLIDE 14

Phase 1

9 Sept 2013 – 9 March 2016

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Phase 1 (Rural) - Lower San Antonio River & Tributaries Station ID Site Description Seg/AU County Lat Long

S0036 UNNAMED TRIBUTARY IMMEDIATELY UPSTREAM OF THE CONFLUENCE WITH THE SAN ANTONIO RIVER 750 METERS DOWNSTREAM OF GOLIAD WWTP DISCHARGE 1901_01 Goliad 28.660482

  • 97.392913

S0037 ESCONDIDO CREEK IMMEDIATELY UPSTREAM OF THE CONFLUENCE WITH THE SAN ANTONIO RIVER 1901A_01 Karnes 28.847796

  • 97.749661

17858 SAN ANTONIO RIVER @ US 59 4.6 KM SOUTHWEST OF GOLIAD, TEXAS 1901_01 Goliad 28.651148

  • 97.43291

12797 CIBOLO CREEK @ FM 81 EAST OF PANNA MARIA 1902_01 Karnes 28.972057

  • 97.874924

12879 SAN ANTONIO RIVER @ FM 791 SW OF FALLS CITY, TEXAS 1911_01 Karnes 28.951504

  • 98.064363
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SLIDE 15

Phase 1 Results

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Station Ambient Samples

  • E. coli

Geomean (cfu/100 mL)

  • Avg. Flow

(cfs) High-Flow Samples

  • E. coli

Geomean (cfu/100 mL)

  • Avg. Flow

(cfs) Goliad Trib. 4 320.6 0.2 2* 5099.0 0.2 SAR @ US 59 4 94.4 280.0 2 2683.3 930.5 Escondido Creek 4 452.6 1.3 2 7071.1 18.2 Cibolo @ FM 81 4 137.7 29.2 2 1805.5 548.0 SAR @ FM 791 4 102.6 195.5 2 1578.0 1256.0 *Sample collected March 9, 2016; results yet to be received

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Phase 1: 3-Way Split

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53.1% 29.7% 7.1% 10.1%

Overall 3-Way Split

Wildlife (n=157) Livestock & Domesticated Animals (n=88) Human (n=21) Unidentified (n=30)

Source classification of E. coli isolates (combined n=296) from 29 combined samples

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SLIDE 17

Phase 1: 7-Way Split

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36.8% 16.2% 9.5% 6.1% 9.8% 7.1% 5.1% 9.5%

Overall 7-Way Split

Non-Avian Wildlife (n=109) Avian Wildlife (n=48) Cattle (n=28) Other Livestock, Avian (n=18) Other Livestock, Non-Avian (n=29) Human (n=21) Pets (n=15) Unidentified (n=28)

Source classification of E. coli isolates (combined n=296) from 29 combined samples

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Phase 1: Species Summary

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42 27 25 23 20 17 17 16 15 15 15

5 10 15 20 25 30 35 40 45

10 Most Common Isolate Origins

Source classification of E. coli isolates (combined n=296) from 29 combined samples

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SLIDE 19

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Phase 2

15 Dec 2014 – 26 Oct 2015

Phase 2 (Rural) - Lower Cibolo Creek & Tributaries Station ID Site Description

Seg/AU County Lat Long 14211 CIBOLO CREEK @ CR 389 NEAR CESTOHOWA, TEXAS 1902_02 Karnes 29.01700 -97.91900 S0060 CLIFTON BRANCH IMMEDIATELY UPSTREAM OF ITS CONFLUENCE WITH CIBOLO CREEK 1902C_01 Wilson 29.20291 -97.99999 S0061 UNNAMED TRIBUTARY OF CIBOLO CREEK 30 METERS UPSTREAM OF CR 401 1902 Wilson 29.21996 -98.00966 12805 CIBOLO CREEK @ FM 539 1902_03 Wilson 29.27977 -98.05329 12741 MARTINEZ CREEK ON NORTH GABLE ROAD SOUTH OF ZUEHL 1902A_01 Bexar 29.44415 -98.16889 12919 CIBOLO CREEK 40 METERS DOWNSTREAM FROM IH 10/US 90 ON EAST BANK 1913_01 Bexar 29.50055 -98.18639

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SLIDE 20

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Station Ambient Samples

  • E. coli

Geomean (cfu/100 mL) Avg. Flow (cfs) High-Flow Samples

  • E. coli

Geomean (cfu/100 mL) Avg. Flow (cfs) Cibolo @ CR 389 3 126.0 54.3 2 9695.4 1020.0 Clifton Branch Confluence 3 470.9 0.17 2 1553.1 0.9 Unnamed Cibolo Tributary 3 551.8 0.2 2 3435.1 0.2 Cibolo @ FM 539 3 249.2 36.3 2 3566.5 423.0 Martinez Creek @ N Gable 3 308.7 12.3 2 3203.1 101.0 Cibolo Down IH-10 3 73.1 21.3 2 358.9 37.0

Phase 2

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SLIDE 21

Cibolo Creek @ CR 389

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July 30, 2015 - 60 cfsApril 13, 2015 – 1020 cfs

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SLIDE 22

Phase 2: 3-Way Split

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52.7% 26.4% 3.9% 17.0%

Overall 3-Way Split

Wildlife (n=164) Livestock & Domesticated Animals (n=82) Human (n=12) Unidentified (n=53)

Source classification of E. coli isolates (combined n=311) from 30 samples

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SLIDE 23

Phase 2: 7-Way Split

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39.9% 12.9% 6.1% 5.8% 7.4% 3.9% 7.1% 17.0%

Overall 7-Way Split

Non-Avian Wildlife (n=124) Avian Wildlife (n=40) Cattle (n=19) Other Livestock, Avian (n=18) Other Livestock, Non-Avian (n=23) Human (n=12) Pets (n=22)

Source classification of E. coli isolates (combined n=311) from 30 samples

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SLIDE 24

Phase 2: Species Summary

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31 30 30 24 20 19 17 16 13 13

5 10 15 20 25 30 35

Feral Hog Cattle Opossum Human Coyote Chicken Goat Duck Fox Dog

10 Most Common Isolate Origins

Source classification of E. coli isolates (combined n=311) from 30 samples

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SLIDE 25

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Basin Wide: 3-Way Summary

53% 28% 5% 14%

Overall 3-Way Split

Wildlife (n=321) Livestock & Domesticated Animals (n=170) Human (n=33) Unidentified (n=83)

Source classification of E. coli isolates (combined n=607) from 59 samples

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SLIDE 26

Basin Wide: 7-Way Summary

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38.4% 14.5% 7.7% 5.9% 8.7% 5.4% 6.1% 13.3%

Overall 7-Way Split

Non-Avian Wildlife (n=233) Avian Wildlife (n=88) Cattle (n=47) Other Livestock, Avian (n=36) Other Livestock, Non-Avian (n=52) Human (n=33) Pets (n=37) Unidentified (n=81)

Source classification of E. coli isolates (combined n=607) from 59 samples

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SLIDE 27

Basin Wide: Species Summary

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72 57 56 42 39 37 32 31 29 28

10 20 30 40 50 60 70 80

10 Most Common Isolate Origins

Source classification of E. coli isolates (combined n=607) from 59 samples

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SLIDE 28

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Phase 3

29 Feb 2016 – 9 March 2016

Phase 3 (Urban) - Upper San Antonio River & Tributaries Station ID Site Description

Seg/AU County Lat Long 12909 SAN ANTONIO RIVER AT MULBERRY STREET 1911_09 Bexar 29.45618

  • 98.47589

12906 SAN ANTONIO RIVER AT PECAN STREET 1911_09 Bexar 29.42864

  • 98.49173

12904 SAN ANTONIO RIVER AT ALAMO STREET 1911_09 Bexar 29.41034

  • 98.49525

12707 SAN PEDRO CREEK AT FURNISH STREET 1911D_01 Bexar 29.40626

  • 98.51049

12897 SAN ANTONIO RIVER AT IH 410 LOW WATER CROSSING CAMINO COAHUILATECHAN 0.25 KM BELOW THE BRIDGE IN SAN ANTONIO 1911_07 Bexar 29.31953

  • 98.44889
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SLIDE 29

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Station Ambient Samples

  • E. coli

(cfu/100 mL) Flow (cfs) High-Flow Samples

  • E. coli

(cfu/100 mL) Flow (cfs) SAR @ Mulberry 1 650 8.7 1 >20000 231 SAR @ Pecan 1 180 8.8 1 12000 <0.01 SAR @ Alamo 1 280 27 1 14000 25 San Pedro Creek @ Furnish 1 140 2.3 1 >20000 135 SAR @ Loop 410 1 240 21 1 >20000 704

Phase 3

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Future of Effort

  • Three additional

Phase 3 samples

  • Pending results will

determine high-flow

  • r ambient collection

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SAR @ Mulberry

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SLIDE 31

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Questions???