Author'Academy:'' Effec0vely$Communica0ng$Your$Research ' - - PowerPoint PPT Presentation

Jeff$Robens,$PhD$ Senior'Editor'

• Author'Academy:''

Effec0vely$Communica0ng$Your$Research'

February'2014'

Download'at:'edanzedi9ng.com/India_2014'

Be an effective communicator

S'

Your'goal'is'not'only'to'be'published,' but'also'to'be'widely'read/cited

! Write$effec0vely$ ! Logically$present$your$research$in$your$manuscript$ ! Choose$the$best$journal$to$reach$your$target$audience$ ! Convey$the$significance$of$your$work$to$journal$editors$ ! Properly$revise$your$manuscript$aFer$peer$review$ ! Promote$your$research$aFer$publica0on

Effec<ve'wri<ng'

Section 1

Download'at:'edanzedi9ng.com/India_2014'

Effec9ve'wri9ng'

Active voice

Sentences$wriHen$in$the$ac0ve$voice$are:$

simple' direct' clear' easy'to'read' The$mechanisms$regula0ng$tumor$growth$were$inves0gated.$

Passive'

We$inves0gated$the$mechanisms$regula0ng$tumor$growth.$

Ac<ve'

Effec9ve'wri9ng'

Active voice

70' 75' 80' 85' 90' 95' 100' Ac9ve' Passive'

Accuracy$(%)$

1500' 1600' 1700' 1800' 1900' 2000' 2100' Ac9ve' Passive'

Decision$0me$(ms)$

Ferreira'F.'Cogni9ve'Psychology'2003;'47:'164–203.'

• 48'na9ve'EnglishPspeaking'university'students'
• 36'ac9ve'voice'sentences'(‘the'dog'bit'the'man’)'
• 36'passive'voice'sentences'(‘the'man'was'biWen'by'the'dog’)

Effec9ve'wri9ng'

Active voice is preferred

“Use'the'ac<ve'voice'when'it'is'less'wordy'and'more' direct'than'the'passive”.'(3rd'ed.,'pg.'42)' “Use'the'ac<ve'voice'rather'than'the'passive'voice…”.''

www.apastyle.org/learn/faqs/effec9vePverbPuse.aspx'

“As'a'maWer'of'style,'passive'voice'is'typically,'but'not' always,'inferior'to'ac<ve'voice”.'(15th'ed.,'pg.'177)' “In'general,'authors'should'use'the'ac<ve'voice…”.'

(10th'ed.,'pg.'320)'

ACS'Style'Guide' APA'Style'' Chicago'Style' Guide' AMA'Manual'of' Style'

Effec9ve'wri9ng'

Stress position

Readers'focus'at'the'end'of'the'sentence'to' determine'what'is'important.'

• 1. You$deserve$a$raise,$but$the$budget$is$0ght.$

Which$sentence$suggests$that$you$$ will$get$a$raise?$

• 2. The$budget$is$0ght,$but$you$deserve$a$raise.$

hWp://wri9ngcenter.unc.edu/handouts/flow/'

Effec9ve'wri9ng'

The$budget$is$0ght,$but$you$deserve$a$raise.$Your$salary$ will$increase$at$the$beginning$of$next$year.$

hWp://wri9ngcenter.unc.edu/handouts/flow/'

Stress$posi0on$ Topic$posi0on$

The'topic'posi<on'introduces'the'idea'of'the'current'sentence'

The$stress$posi0on$also$introduces$$ the$topic$of$the$next$sentence$

Stress position

Effec9ve'wri9ng'

Topic position

The' pa<ent' went$ to$ the$ hospital$ to$ see$ a$ gastroenterologist.$ The' doctor' then$ performed$ a$ series$ of$ diagnos<c' tests.$ The' results' showed$ the$ pa0ent$suffered$from$a$bacterial'infec<on.'An<bio<cs' were$ prescribed$ to$ treat$ the$ infec0on$ before$ the$ pa0ent$developed$an$ulcer.$$

idea idea idea idea Topic'link sentence

Effec9ve'wri9ng'

Busch'et'al.'BMC!Cancer.'2012;'13:'211.'

Linking your ideas in your manuscript

Lung' cancer' is' the' leading' cause' of' cancer' mortality' for' men' and' women.' Despite' smoking' preven9on' and' cessa9on' programs' and' advances' in' early' detec9on,' the' 5Pyear' survival' rate' for' lung' cancer' is' only' 16%' with' current' therapies.'Although'lung'cancer'incidence'rates'have'recently'declined'in'the' United'States,'more'lung'cancer'is'now'diagnosed'when'considered'together'in' formerP' and' neverPsmokers' than' in' current' smokers.' Thus,' even' if' all' of' the' na9onal'an9Psmoking'campaign'goals'are'met,'lung'cancer'will'remain'a'major' public'health'problem'for'decades.'New'ways'to'treat'or'prevent'lung'cancer' are'therefore'needed.' ' ' One'poten9al'therapeu9c'target'for'lung'cancer'is'the'Wnt'signaling'pathway.' The' canonical' Wnt' signaling' pathway' in' mammals' consists' of' a' family' of' secreted' lipidPmodified' Wnt' protein' ligands' that' bind' to' a' family' of' 7Ppass' transmembrane'Frizzled'(Fzd)'receptors,'as'reviewed…'

Effec9ve'wri9ng'

Busch'et'al.'BMC!Cancer.'2012;'13:'211.'

Lung' cancer' is' the' leading' cause' of' cancer' mortality' for' men' and' women.' Despite' smoking' preven9on' and' cessa9on' programs' and' advances' in' early' detec9on,' the' 5Pyear' survival' rate' for' lung' cancer' is' only' 16%' with' current' therapies.'Although'lung'cancer'incidence'rates'have'recently'declined'in'the' United'States,'more'lung'cancer'is'now'diagnosed'when'considered'together'in' formerP' and' neverPsmokers' than' in' current' smokers.' Thus,' even' if' all' of' the' na9onal'an9Psmoking'campaign'goals'are'met,'lung'cancer'will'remain'a'major' public'health'problem'for'decades.'New'ways'to'treat'or'prevent'lung'cancer' are'therefore'needed.' ' ' One'poten9al'therapeu9c'target'for'lung'cancer'is'the'Wnt'signaling'pathway.' The' canonical' Wnt' signaling' pathway' in' mammals' consists' of' a' family' of' secreted' lipidPmodified' Wnt' protein' ligands' that' bind' to' a' family' of' 7Ppass' transmembrane'Frizzled'(Fzd)'receptors,'as'reviewed…'

Topic$sentence$

Linking your ideas in your manuscript

Effec9ve'wri9ng'

Busch'et'al.'BMC!Cancer.'2012;'13:'211.'

Lung' cancer' is' the' leading' cause' of' cancer' mortality' for' men' and' women.' Despite' smoking' preven9on' and' cessa9on' programs' and' advances' in' early' detec9on,' the' 5Pyear' survival' rate' for' lung' cancer' is' only' 16%' with' current' therapies.'Although'lung'cancer'incidence'rates'have'recently'declined'in'the' United'States,'more'lung'cancer'is'now'diagnosed'when'considered'together'in' formerP' and' neverPsmokers' than' in' current' smokers.' Thus,' even' if' all' of' the' na9onal'an9Psmoking'campaign'goals'are'met,'lung'cancer'will'remain'a'major' public'health'problem'for'decades.'New'ways'to'treat'or'prevent'lung'cancer' are'therefore'needed.' ' ' One'poten9al'therapeu9c'target'for'lung'cancer'is'the'Wnt'signaling'pathway.' The' canonical' Wnt' signaling' pathway' in' mammals' consists' of' a' family' of' secreted' lipidPmodified' Wnt' protein' ligands' that' bind' to' a' family' of' 7Ppass' transmembrane'Frizzled'(Fzd)'receptors,'as'reviewed…'

Topic$sentence$ Stress$sentence$

Linking your ideas in your manuscript

Effec9ve'wri9ng'

Busch'et'al.'BMC!Cancer.'2012;'13:'211.'

Lung' cancer' is' the' leading' cause' of' cancer' mortality' for' men' and' women.' Despite' smoking' preven9on' and' cessa9on' programs' and' advances' in' early' detec9on,' the' 5Pyear' survival' rate' for' lung' cancer' is' only' 16%' with' current' therapies.'Although'lung'cancer'incidence'rates'have'recently'declined'in'the' United'States,'more'lung'cancer'is'now'diagnosed'when'considered'together'in' formerP' and' neverPsmokers' than' in' current' smokers.' Thus,' even' if' all' of' the' na9onal'an9Psmoking'campaign'goals'are'met,'lung'cancer'will'remain'a'major' public'health'problem'for'decades.'New'ways'to'treat'or'prevent'lung'cancer' are'therefore'needed.' ' ' One'poten9al'therapeu9c'target'for'lung'cancer'is'the'Wnt'signaling'pathway.' The' canonical' Wnt' signaling' pathway' in' mammals' consists' of' a' family' of' secreted' lipidPmodified' Wnt' protein' ligands' that' bind' to' a' family' of' 7Ppass' transmembrane'Frizzled'(Fzd)'receptors,'as'reviewed…'

Topic$sentence$ Stress$sentence$ Topic$sentence$

Use'the'topic'and'stress'posi<ons'to'logically' present'your'ideas'within'your'manuscript'

Linking your ideas in your manuscript

Manuscript'structure'

Section 2

Download'at:'edanzedi9ng.com/India_2014'

Coverage'and' Staffing'Plan' Manuscript' structure'

Introduction

General$introduc0on$ Specific$aims$ Aims' Current$state$of$the$field$ Problem$in$the$field$

Coverage'and' Staffing'Plan' Manuscript' structure'

Introduction – flow of information

Lung'cancer'is'the'leading'cause'of'cancer'mortality'for'men'and'women.'Despite'smoking'preven9on'and'cessa9on'programs'and'advances'in'early' detec9on,'the'5Pyear'survival'rate'for'lung'cancer'is'only'16%'with'current'therapies.'Although'lung'cancer'incidence'rates'have'recently'declined'in'the' United'States,'more'lung'cancer'is'now'diagnosed'when'considered'together'in'formerP'and'neverPsmokers'than'in'current'smokers.'Thus,'even'if'all'of'the' na9onal'an9Psmoking'campaign'goals'are'met,'lung'cancer'will'remain'a'major'public'health'problem'for'decades.'New'ways'to'treat'or'prevent'lung'cancer' are'therefore'needed.' ' One'poten9al'therapeu9c'target'for'lung'cancer'is'the'Wnt'signaling'pathway.'The'canonical'Wnt'signaling'pathway'in'mammals'consists'of'a'family'of' secreted'lipidPmodified'Wnt'protein'ligands'that'bind'to'a'family'of'7Ppass'transmembrane'Frizzled'(Fzd)'receptors,'as'reviewed.'In'brief,'in'the'absence'of' ligand,'glycogen'synthase'kinaseP3'(GSK3),'in'complex'with'axin'and'adenomatous'polyposis'coli'(APC),'cons9tu9vely'phosphorylates'βPcatenin,'the'primary' Wnt' signaling' effector,' targe9ng' it' for' ubiqui9na9on' and' proteasomal' destruc9on.' Ligand' binding' engages' a' pathway' involving' Dishevelled' (Dvl)' that' inhibits'GSK3,'allowing'βPcatenin'to'accumulate'in'a'hypophosphorylated'form.'This'stabilized'form'of'βPcatenin'can'translocate'to'the'nucleus,'where'it' ac9vates'target'gene'transcrip9on'by'complexing'with'T'cell'factor'(TCF)'and'lymphoid'enhancerPbinding'factor'(LEF).'In'addi9on'to'key'mediators'of' embryonic'development,'these'target'genes'include'cri9cal'growthPregulators'such'as'myc'and'cyclin!D1.'' ' Aberrant'Wnt'signaling'due'to'muta9ons'in'β+catenin'or'APC'drives'deregulated'growth'in'both'familial'and'nonPhereditary'colorectal'cancers.'However,' nonPsmall'cell'lung'cancers'(NSCLC),'the'most'common'type'of'lung'cancer,'rarely'harbor'APC'or'β+catenin'muta9ons.'Rather,'aberrant'Wnt'ac9vity'in'lung' cancer'is'linked'to'increased'expression'of'upstream'Wnt'signaling'effectors'such'as'Dvl'or'decreased'expression'of'Wnt'antagonists'such'as'WntPinhibitory' factor'1'(WifP1).'' ' Effec9ve'pharmacological'inhibitors'of'the'Wnt'pathway'have'only'recently'become'available.'Screens'for'smallPmolecule'antagonists'of'the'Wnt'pathway' found'two'enzymes'to'be'key'mediators'of'Wnt'signaling.'These'are'polyPADPPribose'polymerase'(PARP)'enzymes,'tankyrase'(TNKS)'1'and'TNKS2,'which' aWach'polyPADPPribose'(PAR)'onto'substrate'proteins.'Their'roles'in'regula9ng'telomerase'func9on'and'mito9c'spindle'forma9on'are'known,'but'their'role' in'PARsyla9ng'axin'so'as'to'maintain'the'op9mal'level'for'canonical'Wnt'signaling'has'only'recently'been'recognized.'The'compounds'iden9fied'in'these' screens,'XAV939,'IWRP1'exo,'and'IWRP1'endo,'act'by'specifically'inhibi9ng'the'PARP'ac9vity'of'TNKS1'and'TNKS2.'IWRPexo'is'a'stereoisomer'of'IWRP1'endo' with' ~14Pfold' lower' EC50.' PARP' inhibi9on' is' a' tractable' pharmacological' target' in! vivo,' as' antagonists' of' other' PARP' homologs' exert' an9neoplas9c' responses'in'breast'and'ovarian'cancer,'as'reviewed.'' ' This'study'explored'the'hypothesis'that'inhibi9on'of'TNKS'by'pharmacological'or'gene9c'means'would'inhibit'lung'cancer'growth$in!vitro'and'in!vivo'in' clinicallyPrelevant'transgenic'mouse'models'of'lung'cancer'that'were'previously'developed,'as'reviewed.' '

Busch'et'al.'BMC!Cancer.'2012;'13:'211.'

General$introduc0on$ Secondary$introduc0on$ Objec0ves$

Coverage'and' Staffing'Plan' Manuscript' structure'

Writing the Introduction

Beginning'should'demonstrate'' relevance/interest'

Lung' cancer' is' the' leading' cause' of' cancer' mortality' for' men' and' women.' Despite' smoking'preven9on'and'cessa9on'programs'and'advances'in'early'detec9on,'the'5Pyear' survival'rate'for'lung'cancer'is'only'16%'with'current'therapies.'Although'lung'cancer' incidence'rates'have'recently'declined'in'the'United'States,'more'lung'cancer'is'now' diagnosed' when' considered' together' in' formerP' and' neverPsmokers' than' in' current' smokers.'Thus,'even'if'all'of'the'na9onal'an9Psmoking'campaign'goals'are'met,'lung' cancer'will'remain'a'major'public'health'problem'for'decades.'New'ways'to'treat'or' prevent'lung'cancer'are'therefore'needed.'

Interest$

Important'problem'in'the'field'

Busch'et'al.'BMC!Cancer.'2012;'13:'211.'

Coverage'and' Staffing'Plan' Manuscript' structure'

Your'aims'should'directly'' address'this'problem'

This' study' explored' the' hypothesis' that' inhibi9on' of' TNKS' by' pharmacological'or'gene9c'means'would'inhibit'lung'cancer'growth$in!vitro' and'in!vivo…'

Writing the Introduction

New$ways$to$treat$or$prevent$lung$cancer$are$therefore$needed.$

Busch'et'al.'BMC!Cancer.'2012;'13:'211.'

Coverage'and' Staffing'Plan' Manuscript' structure'

Methods

How$it$was$ done$

General$methods$ Specific$techniques$ (discuss$controls)$ Data$analysis$ Quan0fica0on$methods$ Sta0s0cal$tests$

What$was$ used$

Samples$or$par0cipants$ Materials$

How$it$was$ analyzed$

Experimental'Design'

Coverage'and' Staffing'Plan' Manuscript' structure'

Results

• 1. Ini0al$observa0on$
• 2. Characteriza0on$
• 3. Applica0on$

Logical$presenta0on$

Example:'

'

• 1. New$gene$expressed$in$the$heart$
• 2. Regula0on$of$gene$expression,$when$it$is$

expressed,$func0on$of$the$produced$protein$

• 3. Role$of$the$gene$in$heart$development$

Coverage'and' Staffing'Plan' Manuscript' structure'

Results

• 1. Ini0al$observa0on$
• 2. Characteriza0on$
• 3. Applica0on$

Each$subsec0on$ corresponds$to$$

• ne'figure$

What$you$found,$not$ what$it$means'

Logical$presenta0on$ Subsec0ons$ Factual$descrip0on$

Coverage'and' Staffing'Plan' Manuscript' structure'

Display items

Present$large$amount$

• f$data$quickly$and$

efficiently' Keep$it$simple:$use$ separate$panels$if$ necessary$ Must$be$able$to$stand' alone:$clear$labels$ and$figure$legends$ Usually$the$first$thing$ readers$will$look$at$

Figures,$graphs$ &$tables'

Coverage'and' Staffing'Plan' Manuscript' structure'

Figures

Clear$figure$legend$

Kindlinb2$knockdown$and$focal$adhesion$localiza0on.' Confocal' immunofluorescent' microscopy' with' an9Pβ1' integrin' and' an9Ppaxillin' on' C2C12' cells' transfected' with' RNAi' and' then' changed' to' differen9a9on' media' for'2'days.'Control'cells'show'linear'staining'consistent' with' localiza9on' to' costameres' (arrows),' as' well' as' punctate' focal' contact' staining' (arrowheads).' Focal' contact'proteins'in'the'kindlinP2'RNAi'cells'fail'to'form' linear' structures' and' instead' are' concentrated' in' unusual'appearing'puncta'(*).'(Scale'bar'='20'μM).''

Dowling'et'al.'(2008)'BMC'Cell'Biol'9:36.'

Clear$indicators$

Title$of$the$experiment$ Brief$methodology$ Key$findings$

Coverage'and' Staffing'Plan' Manuscript' structure'

Discussion

Summary$of$findings$ Relevance$of$ findings$ Implica0ons$for$$ the$field$

Coverage'and' Staffing'Plan' Manuscript' structure'

GPER'is'an'E2'binding,'GPprotein'coupled'membrane'receptor'that'was'reported'to'be'overexpressed'in'breast'endometrial,'ovarian''and'thyroid'cancers.'The' results'presented'here'extend'these'observa9ons'to'show'that'different'types'of'lung'cancers'including'adenocarcinomas,'squamous'cell'carcinoma'and'large' cell'carcinomas'express'higher'GPER'than'normal'lung'9ssue.' 'Here,'we'demonstrate'for'the'first'9me'that'GPER'is'overexpressed'in'lung'tumors'and'lung' adenocarcinoma'cell'lines'rela9ve'to'normal'lung'and'immortalized'normal'lung'cell'lines,'although'the'expression'of'GPER'transcript'in'HPL1D'cells'is'higher' than'HBECs.'' ' GPER'has'been'postulated'to'be'involved'in'E2Pac9va9on'of'EGFR.'Filardo’s'group'showed'a'link'between'GPER'expression'and'tumor'progression'and'increased' tumor' size' in' breast' cancer' pa9ents.' Recently,' GPER' overexpression' was' reported' to' be' independent' of' ERα' expression' in' breast' cancer' pa9ent' samples,' indica9ng'the'importance'of'GPER'in'ERα'nega9ve'tumors.'GPER'and'EGFR'expression'were'correlated'in'endometrial'adenocarcinoma.'Further,'overexpression'

• f' GPER' in' advanced' stage' endometrial' adenocarcinoma' correlated' with' poor' survival.' Other' studies' also' suggest' increased' GPER' in' breast,' ovarian' and'

endometrial' cancers' correlates' with' disease' severity' and' reduced' survival.' These' results' are' in' agreement' with' studies' demonstra9ng' associa9on' of' GPER'

• verexpression'in'other'cancers,'although'the'scoring'paWerns'and'correla9on'of'expression'levels'to'disease'state'may'vary'among'these'studies.''

' A' limita9on' of' our' study' is' that' the' average' GPER' staining' scores' among' different' lung' cancer' grades' (I' (10' cases),' II' (30' cases),' III' (16' cases))' were' not' significantly'different.'One'other'limita9on'of'the'current'study'is'that'we'cannot'conclude'at'this'9me'whether'GPER'overexpression'is'cause'or'consequence'of' cancer.'It'is'also'possible'that'overexpression'of'GPER'in'lung'cancers'may'reflect'a'defense'mechanism'to'counteract'excessive'prolifera9on.'Indeed,'a'recent' report'by'Krakstad'et!al.'showed'that'loss'of'GPER'in'ERαPposi9ve'endometrial'cancers'is'associated'with'poor'prognosis.'Another'study'showed'that'the'GPER' agonist' GP1' inhibited' E2Pinduced' uterine' epithelial' cell' prolifera9on' in' mice' by' repressing' MAPK' ac9va9on,' indica9ng' that' GPER' effects' are' 9ssue' specific.' Because'our'studies'were'performed'on'commercial'TMAs,'the'results'cannot'be'extrapolated'to'correlate'GPER'expression'levels'to'disease'outcomes.'Clearly,' this'is'a'next'logical'step'in'light'of'the'novel'findings.'' ' We'observed'no'differences'in'GPER'expression'between'adenocarcinoma'cell'lines'or'tumors'from'male'and'female'pa9ents,'similar'to'the'previous'findings'of' no'difference'in'ERα'or'ERβ'expression'in'NSCLC'cells'and'tumors'based'on'gender.'In'Western'blots,'rather'than'rely'on'one'GPER'an9body'in'our'study,'we' used'3'different'commercial'an9bodies'to'determine'the'correla9on'between'mRNA'and'protein'levels.'It'is'indeed'evident'from'our'Western'blot'data'that' GPER'appears'to'have'different'MW'forms,'likely'due'to'glycosyla9on,'dimeriza9on,'and'interac9on'with'other'membrane'proteins,'and'levels'in'the'lung' adenocarcinoma'cell'lines.'More'trivial'explana9ons'for'the'different'staining'paWerns'of'GPER'in'Western'blots'may'be'due'to'differen9al'purity/affinity'of'the' three'GPER'an9bodies'as'well'as'their'capacity'to'bind'to'secondary'an9bodies.'It'will'be'important'to'determine'the'nature'of'these'forms'by'proteomic'analysis' and'gene'sequencing'to'evaluate'their'biological'significance.'' ' MechanismPbased'studies'showed'that'GPER'transac9vates'EGFR'in'breast'cancer'cells''as'well'as'in'thyroid,'endometrial'and'ovarian'cancer'cell'lines.'Inhibitors'

• f'EGFR'tyrosine'kinase'(gefi9nib)'and'ER'(fulvestrant,'ICI'182,780)'were'reported'to'synergize'their'an9Pprolifera9ve'effects'in'NSCLC'.'Given'the'importance'of'

EGFR'signaling'as'a'therapeu9c'target'in'lung'cancer,'further'examina9on'of'the'effect'of'EGF,'heregulin,'and'amphiregulin'on'GPER'expression'and'func9on'in' lung'cancer'may'provide'new'insights'into'resistance'to'EGFR'inhibitors'and'or'how'estrogens's9mulate'lung'cancer.'' ' In'conclusion,'the'data'presented'in'this'manuscript'demonstrate'that'GPER'expression'is'higher'in'lung'tumors'compared'to'normal'lung'9ssue.'While'it'is'not' yet'clear'that'elevated'GPER'expression'is'a'cause'of'or'consequence'from'lung'cancer'progression.'Func9onal'analysis'of'the'effect'of'GPER'expression'will' facilitate'further'delinea9on'of'the'role'of'GPER'in'lung'cancer.'' '

Rao'Jala'et'al.'BMC!Cancer!2012;12:624.'

Discussion – flow of information

Summary'

Major$conclusion$

Relevance'

Previous$studies$ Limita0ons$ Unexpected$results$

Conclusions'

Implica0ons$ Major$conclusion$

Coverage'and' Staffing'Plan' Manuscript' structure'

GPER'is'an'E2'binding,'GPprotein'coupled'membrane'receptor'that'was'reported'to' be'overexpressed'in'breast,'endometrial,'ovarian''and'thyroid'cancers.'The'results' presented' here' extend' these' observa9ons' to' show' that' different' types' of' lung' cancers' including' adenocarcinomas,' squamous' cell' carcinoma' and' large' cell' carcinomas'express'higher'GPER'than'normal'lung'9ssue.' 'Here,'we'demonstrate' for' the' first' 9me' that' GPER' is' overexpressed' in' lung' tumors' and' lung' adenocarcinoma'cell'lines'rela9ve'to'normal'lung'and'immortalized'normal'lung' cell'lines,'although'the'expression'of'GPER'transcript'in'HPL1D'cells'is'higher'than' HBECs.''

Rao'Jala'et'al.'BMC!Cancer!2012;12:624.'

Discussion – flow of information

Beginning'should'state'the'major' conclusion'of'the'study'

RePintroduc9on' Conclusion'

Coverage'and' Staffing'Plan' Manuscript' structure'

In' conclusion,' the' data' presented' in' this' manuscript' demonstrate' that' GPER' expression'is'higher'in'lung'tumors'compared'to'normal'lung'9ssue.'While'it'is'not' yet'clear'that'elevated'GPER'expression'is'a'cause'of'or'consequence'from'lung' cancer' progression.' Func9onal' analysis' of' the' effect' of' GPER' expression' will' facilitate'further'delinea9on'of'the'role'of'GPER'in'lung'cancer.''

Rao'Jala'et'al.'BMC!Cancer!2012;12:624.'

Discussion – flow of information

End'should'summarize'your'conclusion' and'clearly'state'the'implica9ons'

RePstate'conclusion' Implica9ons' Future'direc9ons'

Coverage'and' Staffing'Plan' Manuscript' structure'

Linking your ideas

General$background$ Objec0ves$ Methodology$ Results$and$figures$ Summary$of$findings$ Implica0ons$for$the$field$ Relevance$of$findings$ Problems$in$the$field$

Logically'link'your'ideas'throughout'your'manuscript'

Current$state$of$the$field$

Introduc0on$ Methods$ Results$ Discussion$

Coverage'and' Staffing'Plan' Manuscript' structure'

Linking your ideas

New$ways$to$treat$or$prevent$lung$cancer$ $are$therefore$needed.$ This$study$explored$the$hypothesis$that$inhibi0on$

• f$TNKS…would$inhibit$lung$cancer$growth…$

Pharmacological$or$gene0c$inhibi0on$of$TNKS1$ and$TNKS2…reduces$lung$cancer$prolifera0on...$$ Problem$ Objec0ves$ Conclusion$

Discussion' Introduc<on'

Busch'et'al.'BMC!Cancer.'2012;13:211.'

Titles'and'abstracts'

Section 3

Download'at:'edanzedi9ng.com/India_2014'

Customer'Service' Titles'and'abstracts'

Important$points$

! Summarize$key$finding$ ! Contains$keywords$ ! Less$than$20$words$

Avoid$

Effective titles

Your'<tle'should'be'a'concise'summary'of'' your'most'important'finding'

" Ques0ons$ " Abbrevia0ons$ " “New”$or$“novel”$

Customer'Service' Titles'and'abstracts'

Abstract

First$impression$

• f$your$paper$

Importance$of$ your$results$ Validity$of$your$ conclusions$ Relevance$of$$ your$aims$ Judge$your$ wri0ng$style$ Probably$only$part$ that$will$be$read$

Customer'Service' Titles'and'abstracts'

Sections of an abstract

Aims$ Background$ Methods$ Results$ Conclusion$ Why$the$study$was$done$(20%)$ Your$hypothesis$(10%)$ Techniques$(10%)$ Most$important$findings$(40%)$ Conclusion/implica0ons$(20%)$

Concise'summary'of'your'research'

Customer'Service' Titles'and'abstracts'

Unstructured abstract

Our'understanding'of'the'mechanisms'by'which'ducts'and'lobules'develop'is'derived'from' model' organisms' and' threePdimensional' (3D)' cell' culture' models' wherein' mammalian' epithelial'cells'undergo'morphogenesis'to'form'mul9cellular'spheres'with'a'hollow'central' lumen.'However,'the'mechanophysical'proper9es'associated'with'epithelial'morphogenesis' are'poorly'understood.'We'performed'mul9dimensional'livePcell'imaging'analysis'to'track'the' morphogene9c' process' star9ng' from' a' single' cell' to' the' development' of' a' mul9cellular,' spherical' structure' composed' of' polarized' epithelial' cells' surrounding' a' hollow' lumen.' We' report'that'in'addi9on'to'ac9vely'maintaining'apicobasal'polarity,'the'structures'underwent' rota9onal'mo9ons'at'rates'of'15–20'μm/h'and'the'structures'rotated'360°'every'4'h'during' the'early'phase'of'morphogenesis.'Rota9onal'mo9on'was'independent'of'the'cell'cycle,'but' was'blocked'by'loss'of'the'epithelial'polarity'proteins'Scribble'or'Pard3,'or'by'inhibi9on'of' dyneinPbased'microtubule'motors.'Interes9ngly,'none'of'the'structures'derived'from'human' cancer' underwent' rota9onal' mo9on.' We' found' a' direct' rela9onship' between' rota9onal' mo9on'and'assembly'of'endogenous'basement'membrane'matrix'around'the'3D'structures,' and'that'structures'that'failed'to'rotate'were'defec9ve'in'weaving'exogenous'laminin'matrix.' Dissolu9on' of' basement' membrane' around' mature,' nonrota9ng' acini' restored' rota9onal' movement' and' the' ability' to' assemble' exogenous' laminin.' Thus,' coordinated' rota9onal' movement'is'a'unique'mechanophysical'process'observed'during'normal'3D'morphogenesis' that'regulates'laminin'matrix'assembly'and'is'lost'in'cancerPderived'epithelial'cells.'

Wang'et'al.'PNAS.'2013;'110:'163‒168.'

Customer'Service' Titles'and'abstracts'

Unstructured abstract

Conclusion$

Thus,'coordinated'rota9onal'movement'is'a'unique'mechanophysical'process'observed'during'normal' 3D'morphogenesis'that'regulates'laminin'matrix'assembly'and'is'lost'in'cancerPderived'epithelial'cells.'

Results$

We' report' that' in' addi9on' to' ac9vely' maintaining' apicobasal' polarity,' the' structures' underwent' rota9onal'mo9ons'at'rates'of'15–20'μm/h'and'the'structures'rotated'360°'every'4'h'during'the'early' phase'of'morphogenesis.'Rota9onal'mo9on'was'independent'of'the'cell'cycle,'but'was'blocked'by'loss'

• f' the' epithelial' polarity' proteins' Scribble' or' Pard3,' or' by' inhibi9on' of' dyneinPbased' microtubule'

motors.'Interes9ngly,'none'of'the'structures'derived'from'human'cancer'underwent'rota9onal'mo9on.' We' found' a' direct' rela9onship' between' rota9onal' mo9on' and' assembly' of' endogenous' basement' membrane'matrix'around'the'3D'structures,'and'that'structures'that'failed'to'rotate'were'defec9ve'in' weaving' exogenous' laminin' matrix.' Dissolu9on' of' basement' membrane' around' mature,' nonrota9ng' acini'restored'rota9onal'movement'and'the'ability'to'assemble'exogenous'laminin.'

Methods$

We'performed'mul9dimensional'livePcell'imaging'analysis'to'track'the'morphogene9c'process'star9ng' from' a' single' cell' to' the' development' of' a' mul9cellular,' spherical' structure' composed' of' polarized' epithelial'cells'surrounding'a'hollow'lumen.''

Background$

Our' understanding' of' the' mechanisms' by' which' ducts' and' lobules' develop' is' derived' from' model'

• rganisms'and'threePdimensional'(3D)'cell'culture'models'wherein'mammalian'epithelial'cells'undergo'

morphogenesis' to' form' mul9cellular' spheres' with' a' hollow' central' lumen.' However,' the' mechanophysical'proper9es'associated'with'epithelial'morphogenesis'are'poorly'understood.''

Wang'et'al.'PNAS.'2013;'110:'163‒168.'

Customer'Service' Titles'and'abstracts'

Writing your abstract

Our'understanding'of'the'mechanisms'by'which'ducts'and'lobules'develop'is'derived'from'model'

• rganisms' and' threePdimensional' (3D)' cell' culture' models' wherein' mammalian' epithelial' cells'

undergo'morphogenesis'to'form'mul9cellular'spheres'with'a'hollow'central'lumen.'However,'the' mechanophysical'proper9es'associated'with'epithelial'morphogenesis'are'poorly'understood.'We' performed'mul9dimensional'livePcell'imaging'analysis'to'track'the'morphogene9c'process'star9ng' from'a'single'cell'to'the'development'of'a'mul9cellular,'spherical'structure'composed'of'polarized' epithelial' cells' surrounding' a' hollow' lumen.' We' report' that' in' addi9on' to' ac9vely' maintaining' apicobasal'polarity,'the'structures'underwent'rota9onal'mo9ons'at'rates'of'15–20'μm/h'and'the' structures'rotated'360°'every'4'h'during'the'early'phase'of'morphogenesis.'Rota9onal'mo9on'was' independent'of'the'cell'cycle,'but'was'blocked'by'loss'of'the'epithelial'polarity'proteins'Scribble'or' Pard3,'or'by'inhibi9on'of'dyneinPbased'microtubule'motors.'Interes9ngly,'none'of'the'structures' derived'from'human'cancer'underwent'rota9onal'mo9on.'We'found'a'direct'rela9onship'between' rota9onal' mo9on' and' assembly' of' endogenous' basement' membrane' matrix' around' the' 3D' structures,'and'that'structures'that'failed'to'rotate'were'defec9ve'in'weaving'exogenous'laminin' matrix.' Dissolu9on' of' basement' membrane' around' mature,' nonrota9ng' acini' restored' rota9onal' movement'and'the'ability'to'assemble'exogenous'laminin.'Thus,'coordinated'rota9onal'movement' is' a' unique' mechanophysical' process' observed' during' normal' 3D' morphogenesis' that' regulates' laminin'matrix'assembly'and'is'lost'in'cancerPderived'epithelial'cells.' Wang'et'al.'PNAS.'2013;'110:'163‒168.'

Customer'Service' Titles'and'abstracts'

Writing your abstract

Our'understanding'of'the'mechanisms'by'which'ducts'and'lobules'develop'is'derived'from'model'

• rganisms' and' threePdimensional' (3D)' cell' culture' models' wherein' mammalian' epithelial' cells'

undergo'morphogenesis'to'form'mul9cellular'spheres'with'a'hollow'central'lumen.'However,'the' mechanophysical'proper9es'associated'with'epithelial'morphogenesis'are'poorly'understood.'We' performed'mul9dimensional'livePcell'imaging'analysis'to'track'the'morphogene9c'process'star9ng' from'a'single'cell'to'the'development'of'a'mul9cellular,'spherical'structure'composed'of'polarized' epithelial' cells' surrounding' a' hollow' lumen.' We' report' that' in' addi9on' to' ac9vely' maintaining' apicobasal'polarity,'the'structures'underwent'rota9onal'mo9ons'at'rates'of'15–20'μm/h'and'the' structures'rotated'360°'every'4'h'during'the'early'phase'of'morphogenesis.'Rota9onal'mo9on'was' independent'of'the'cell'cycle,'but'was'blocked'by'loss'of'the'epithelial'polarity'proteins'Scribble'or' Pard3,'or'by'inhibi9on'of'dyneinPbased'microtubule'motors.'Interes9ngly,'none'of'the'structures' derived'from'human'cancer'underwent'rota9onal'mo9on.'We'found'a'direct'rela9onship'between' rota9onal' mo9on' and' assembly' of' endogenous' basement' membrane' matrix' around' the' 3D' structures,'and'that'structures'that'failed'to'rotate'were'defec9ve'in'weaving'exogenous'laminin' matrix.' Dissolu9on' of' basement' membrane' around' mature,' nonrota9ng' acini' restored' rota9onal' movement'and'the'ability'to'assemble'exogenous'laminin.'Thus,'coordinated'rota9onal'movement' is' a' unique' mechanophysical' process' observed' during' normal' 3D' morphogenesis' that' regulates' laminin'matrix'assembly'and'is'lost'in'cancerPderived'epithelial'cells.'

Conclusions$ Results$ Methods$ Background$

Wang'et'al.'PNAS.'2013;'110:'163‒168.'

Customer'Service' Titles'and'abstracts'

Link ideas in your abstract

Conclusion$

Thus,'coordinated'rota9onal'movement'is'a'unique'mechanophysical'process'observed'during'normal' 3D'morphogenesis'that'regulates'laminin'matrix'assembly'and'is'lost'in'cancerPderived'epithelial'cells.'

Background$

Our' understanding' of' the' mechanisms' by' which' ducts' and' lobules' develop' is' derived' from' model'

• rganisms'and'threePdimensional'(3D)'cell'culture'models'wherein'mammalian'epithelial'cells'undergo'

morphogenesis' to' form' mul9cellular' spheres' with' a' hollow' central' lumen.' However,' the' mechanophysical'proper9es'associated'with'epithelial'morphogenesis'are'poorly'understood.''

Wang'et'al.'PNAS'2013;''110:163‒168.'

However,$the$mechanophysical$proper0es$associated$ with$epithelial$morphogenesis$are$poorly$undersood.$ Thus,$coordinated$rota0onal$movement$is$a$unique$ mechanophysical$process…$ Problem$ Answer$

Thank you!

Any questions?

Follow$us$on$TwiHer$

@JournalAdvisor'

Like$us$on$Facebook$

facebook.com/JournalAdvisor'

Download$and$further$reading$

edanzedi0ng.com/India_2014$

Jeffrey$Robens:$jrobens@edanzgroup.com$

www.edanzedi0ng.com$

Journal'selec<on'

Section 4

Download'at:'edanzedi9ng.com/India_2014'

Journal'selec9on'

Timing

Choose$your$target$journal:'

– A9er'you'have'decided'you'have'enough' results'for'a'publica9on'' – A9er'you'have'decided'on'how'high'to'aim— high,'medium'or'low'impact' – A9er'you'have'decided'how'broadly'relevant' your'findings'are' – Before'wri9ng'the'Title,'Abstract,'Introduc9on'

• r'Discussion'sec9ons'

Journal'selec9on'

Factors to consider when choosing a journal

Aims$&$scope$ Readership$ Open$access$

Which'factor'is'most'important'to'you?'

Impact$factor$

Varies'by'field'

Journal'selec9on' Choosing a target journal

Significance$ Aims$and$Scope$ Impact$

'Journal'selec9on'must'be'based'on'an'' honest'evalua9on'of'your'findings'

Journal'selec9on'

Evaluating significance

How$new$are$your$findings?$ Novelty$ How$broadly$relevant$are$your$findings?$ Relevance$ What$are$the$important$realbworld$ applica0ons?$ Appeal$

Journal'selec9on' Insert$your$proposed$abstract$

Journal Selector –

www.edanzediting.com/journal_selector

Journal'selec9on' Recommended$journals$ Filter$by:$ Impact$factor$ Publishing$frequency$ Open$access$

Journal Selector –

www.edanzediting.com/journal_selector

Journal'selec9on' Seman0c$matching$terms$ Journals$IF,$Aims$&$Scope,$$ and$Frequency$ Similar$published$ar0cles$

Have$they$published$similar$ar0cles$recently?$ Have$you$cited$some$of$these$ar0cles?$

Journal Selector –

www.edanzediting.com/journal_selector

Journal'selec9on' Visit journal websites

Journal'selec9on'

Tips to identify the most suitable journal

S'

Iden0fy$the$ interests$of$the$ journal'editor' Iden0fy$the$ interests$of$the$ readers'

• Editorials$
• Review$ar0cles$
• Special$issues$
• Most$viewed$
• Most$cited$

Cover'leVers'

Section 5

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Coverage'and' Staffing'Plan' Cover'leWers' Abstract:$ First$impression$for$readers$

Cover$leHers$are$the$first$impression$for$ the$journal$editor$

Significance$ Relevance$ Level$of$English$ Interes0ng$to$ their$readers?$ Is$your$work$ important?$

Coverage'and' Staffing'Plan' Cover'leWers'

Dear'Dr'Graeber,' Please' find' enclosed' our' manuscript' en9tled' “AmyloidPlike' inclusions' in' the' brains' of' Hun9ngton’s' disease' pa9ents”,' by ' McGowan'et'al.,'which'we'would'like'to'submit'for'publica9on'as'a'Research'Paper'in'Neurogene>cs.'' Recent'immunohistochemical'studies'have'revealed'the'presence'of'neuronal'inclusions'containing'an'NPterminal'por9on'of ' the'mutant'hun9ng9n'protein'and'ubiqui9n'in'the'brain'9ssues'of'Hun9ngton’s'disease'(HD)'pa9ents;'however,'the'role'of ' these' inclusions' in' the' disease' process' has' remained' unclear.' One' suspected' diseasePcausing' mechanism' in' Hun9ngton’s ' disease'and'other'polyglutamine'disorders'is'the'poten9al'for'the'mutant'protein'to'undergo'a'conforma9onal'change'to'a ' more'stable'an9Pparallel'βPsheet'structure…' To'confirm'if'the'immunohistochemically'observed'hun9ng9nP'and'ubiqui9nPcontaining'inclusions'display'amyloid'features,'we ' performed'Congo'red'staining'and'both'polarizing'and'confocal'microscopy'on'postPmortem'human'brain'9ssues'obtained ' from'five'HD'pa9ents,'two'AD'pa9ents,'and'two'normal'controls.'Congo'red'staining'revealed'a'small'number'of'amyloidPlike ' inclusions' showing' green' birefringence' by' polarized' microscopy,' in' a' variety' of' cor9cal' regions....' ….detected' inclusions '

• bserved' in' parallel' sec9ons,' sugges9ng' that' only' a' rela9vely' small' propor9on' of' inclusions' in' HD' adopt' an' amyloidPlike

' structure.' ' We' believe' our' findings' will' be' of' par9cular' interest' to' the' readership' of' Neurogene>cs,! which' includes' researchers' and ' clinicians'studying'the'gene9c'and'molecular'mechanisms'underlying'neurodegenera9ve'diseases.'Therefore,'we'feel'that'your ' journal'provides'the'most'suitable'pla}orm'for'the'dissemina9on'of'our'work'to'the'research'community.'

Give$the$ background$to$ the$research$ What$was$ done$and$what$ was$$found$

Interest$to$ journal’s$readers$

A good cover letter

We'would'also'like'to'suggest'the'following'reviewers'for'our'manuscript…

• Editor’s$name

Manuscript$0tle Publica0on$type Recommend$reviewers

“MustXhave”'statements'

Coverage'and' Staffing'Plan' Cover'leWers'

“Mustbhave”$ statements$

Not$submiHed$to$

• ther$journals$

Source$of$ funding$ Authors$agree$on$ paper/journal$ Original$and$ unpublished$ No$conflicts$of$ interest$ $Authorship$ contribu0ons$

Disclaimers about publication ethics

Coverage'and' Staffing'Plan' Cover'leWers'

Recommending reviewers

“When' submi~ng' your' paper,' you' must' provide' the' names,'affilia9ons,'and'valid'ePmail'addresses'of'five'(5)' reviewers.' If' you' do' not' do' so,' your' paper' will' be' returned,'unreviewed.”' “Authors' are' requested' to' provide' the' names' and' full' addresses'(including'ePmail'address)'of'up'to'four'poten9al' referees…”'

Coverage'and' Staffing'Plan' Cover'leWers'

Recommending reviewers

Where$to$find$ them?$ From$your$reading/references,$ networking$at$conferences$ How$senior?$ Aim$for$midblevel$researchers$ Who$to$avoid?$ Collaborators$(past$5$years),$ researchers$from$same$ins0tu0on$ Interna<onal'list:$$

1$or$2$from$Asia,$1$or$2$from$Europe,$and$1$or$2$from$North$America$

Peer'Review'

Section 6

Peer'review'

What reviewers are looking for

The$science$ The$manuscript$

! Relevant$hypothesis$ ! Good$experimental$design$ ! Appropriate$methodology$ ! Good$data$analysis$ ! Valid$conclusions$ ! Logical$flow$of$informa0on$ ! Manuscript$structure$and$formapng$ ! Appropriate$references$ ! High$readability$

Abstract'and'Introduc<on' Methods' Results'and'Figures' Discussion'

Peer'review'

Response$ leHer$

Respond$to$every$reviewer$comment$

Easy$to$see$ changes$ Refer$to$line$and$page$numbers$ Use$a$different$color$font$ Highlight$the$text$

Revision

Peer'review'

Writing a response letter

Marc'Lippman,'MD' EditorPinPChief' Breast!Cancer!Research!and!Treatment' ' 3'September'2013' ' Dear'Dr'Lippman,' ' Re:'Resubmission'of'manuscript'reference'No.'WJSP07P5739' ' Please'find'aWached'a'revised'version'of'our'manuscript'originally'en9tled'“Evalua9on'of'the'Glasgow'prognos9c' score'in'pa9ents'undergoing'cura9ve'resec9on'for'breast'cancer'liver'metastases,”'which'we'would'like'to' resubmit'for'considera9on'for'publica9on'in'the'Breast!Cancer!Research!and!Treatment.' ' The'reviewer’s'comments'were'highly'insigh}ul'and'enabled'us'to'greatly'improve'the'quality'of'our'manuscript.'In' the'following'pages'are'our'pointPbyPpoint'responses'to'each'of'the'comments.' ' Revisions'in'the'manuscript'are'shown'as'underlined'text.'In'accordance'with'the'first'comment,'the'9tle'has'been' revised'and'the'en9re'manuscript'has'undergone'substan9al'English'edi9ng.'' We'hope'that'the'revisions'in'the'manuscript'and'our'accompanying'responses'will'be'sufficient'to'make'our' manuscript'suitable'for'publica9on'in'the'Breast!Cancer!Research!and!Treatment.'

Address$editor$personally$ Manuscript$ID$number$ Thank$reviewers$ Highlight$major$changes$

Peer'review'

Agreeing with reviewers

Reviewer'Comment:'In!your!analysis!of!the!data!you!have!chosen! to! use! a! somewhat! obscure! fiIng! func>on! (regression).! ! In! my!

• pinion,!a!simple!Gaussian!func>on!would!have!sufficed.!Moreover,!

the! results! would! be! more! instruc>ve! and! easier! to! compare! to! previous!results.' Response:' We' agree' with' the' reviewer’s' assessment' of' the' analysis.'Our'tailored'func9on,'in'its'current'form,'makes'it'difficult' to' tell' that' this' measurement' cons9tutes' a' significant' improvement' over' previously' reported' values.' We' describe' our' new'analysis'using'a'Gaussian'fi~ng'func9on'in'our'revised'Results' sec9on'(Page'6,'Lines'12–18).'

Peer'review' Reviewer'Comment:'In!your!analysis!of!the!data!you!have!chosen! to! use! a! somewhat! obscure! fiIng! func>on! (regression).! ! In! my!

• pinion,!a!simple!Gaussian!func>on!would!have!sufficed.!Moreover,!

the! results! would! be! more! instruc>ve! and! easier! to! compare! to! previous!results.' Response:' We' agree' with' the' reviewer’s' assessment' of' the' analysis.'Our'tailored'func9on,'in'its'current'form,'makes'it'difficult' to' tell' that' this' measurement' cons9tutes' a' significant' improvement' over' previously' reported' values.' We' describe' our' new'analysis'using'a'Gaussian'fi~ng'func9on'in'our'revised'Results' sec9on'(Page'6,'Lines'12–18).'

Agreeing with reviewers

Agreement$ Revisions$ Loca0on$

Peer'review'

Disagreeing with reviewers

Reviewer'Comment:'In!your!analysis!of!the!data!you!have!chosen! to! use! a! somewhat! obscure! fiIng! func>on! (regression).! ! In! my!

• pinion,! a! simple! Gaussian! func>on! would! have! sufficed.!

Moreover,! the! results! would! be! more! instruc>ve! and! easier! to! compare!to!previous!results.' Response:' Although' a' simple' Gaussian' fit' would' facilitate' comparison'with'the'results'of'other'studies,'our'tailored'func9on' allows' for' the' analysis' of' the' data' in' terms' of' the' Smith' model' [Smith' et' al.,' 1998].' We' have' now' explained' the' use' of' this' func9on' and' the' Smith' model' in' our' revised' Discussion' sec9on' (Page'12,'Lines'2–6).'

Peer'review' Reviewer'Comment:'In!your!analysis!of!the!data!you!have!chosen! to! use! a! somewhat! obscure! fiIng! func>on! (regression).! ! In! my!

• pinion,! a! simple! Gaussian! func>on! would! have! sufficed.!

Moreover,! the! results! would! be! more! instruc>ve! and! easier! to! compare!to!previous!results.' Response:' Although' a' simple' Gaussian' fit' would' facilitate' comparison'with'the'results'of'other'studies,'our'tailored'func9on' allows' for' the' analysis' of' the' data' in' terms' of' the' Smith' model' [Smith' et' al.,' 1998].' We' have' now' explained' the' use' of' this' func9on' and' the' Smith' model' in' our' revised' Discussion' sec9on' (Page'12,'Lines'2–6).' Revisions$ Loca0on$ Evidence$

Disagreeing with reviewers

Peer'review'

“Unfair” reviewer comments

Reviewer'comment:!Currently,!the!authors’!conclusion!that!this! gene!is!involved!in!heart!development!is!not!completely!validated! by! their! in! vitro! analyses.! They! should! do! addi>onal! in! vivo! experiments! using! a! gene>c! mouse! model! to! show! that! heart! development!is!regulated!by!this!gene.!

Reasons'why'reviewers'might'make'these'comments'

'

" Current$results$are$not$appropriate$for$the$impact$factor$of$ the$journal$ " Reviewer$is$being$“unfair”$

Peer'review'

“Unfair” reviewer comments

What'you'should'do'

" Do$the$experiments,$revise,$and$resubmit$to$the$same$ journal$ " Withdraw$submission$and$resubmit$current$ manuscript$to$a$lower$impact$factor$journal$ " Contact$the$journal$editor$if$you$feel$reviewer$is$being$ unfair$

Peer'review'

If rejected, what should you do?

Op0on$1:$New$submission$to$the$same'journal$

'

" Fully$revise$manuscript$ " Prepare$pointbbybpoint$responses$ " Include$the$original$manuscript$ID$number$

Op0on$2:$New$submission$to$a$different'journal$

'

" Revise$manuscript$ " Reformat$according$to$the$author$guidelines$

If accepted, what’s next?

" $Promote$your$work$on$social$networks$$

• 'TwiWer,'LinkedIn,'Research'Gate'

" $Respond$to$postbpublica0on$comments$ " $Present$your$work$at$conferences$

• Promote'your'publica9on'
• Allows'you'to'discuss'your'work'personally'with'your'peers'
• Get'feedback'about'your'work'and'future'direc9ons'

Be an effective communicator

S'

Your'goal'is'not'only'to'be'published,' but'also'to'be'widely'read/cited

! Write$effec0vely$ ! Logically$present$your$research$in$your$manuscript$ ! Choose$the$best$journal$to$reach$your$target$audience$ ! Convey$the$significance$of$your$work$to$journal$editors$ ! Properly$revise$your$manuscript$aFer$peer$review$ ! Promote$your$research$aFer$publica0on

Thank you!

Any questions?

Follow$us$on$TwiHer$

@JournalAdvisor'

Like$us$on$Facebook$

facebook.com/JournalAdvisor'

Download$and$further$reading$

edanzedi0ng.com/India_2014$

Jeffrey$Robens:$jrobens@edanzgroup.com$

www.edanzedi0ng.com$