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METHODS FOR MEASURING THE ANTIMICROBIAL, MECHANICAL, AND PERMEABILITY PROPERTIES OF A BIO-BASED MULTILAYER ANTIMICROBIAL FILM Hunter Gartner April 8 th , 2014 INTRODUCTION Current Research Methods Antimicrobial Mechanical

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  1. METHODS FOR MEASURING THE ANTIMICROBIAL, MECHANICAL, AND PERMEABILITY PROPERTIES OF A BIO-BASED MULTILAYER ANTIMICROBIAL FILM Hunter Gartner April 8 th , 2014

  2. INTRODUCTION  Current Research  Methods  Antimicrobial  Mechanical  Permeability  Conclusions

  3. CURRENT RESEARCH  Development of a completely bio-based and biodegradable antimicrobial package for minimally processed food products  Polylactic acid (PLA)  Chitosan (CS)  Grapefruit Seed Extract (GSE)

  4. PLA/CS COATING

  5. CS COATING

  6. PACKAGING APPLICATION Fres esh Dic ice e Tomatoes oes Filling ng Sealing ing Storage age

  7. METHODS  Antimicrobial  Dynamic shake flasks (ASTM E2149)  Unsuitable for ASTM E2149 because of zone inhibition  Not representative of actual use conditions  No available nutrients for microorganisms to grow  Minimum inhibitory concentration (MIC)  Agar diffusion  Inoculation of 10 4 CFU/mL in tomato juice

  8. METHODS  Mechanical  ASTM D882 Standard test method for tensile properties of thin plastic sheeting  Equipment  Instron Universal Testing Machine

  9. METHODS  Mechanical  ISO 4624 Pull-off test for adhesion  ASTM D4541 Standard test method for pull-off strength of coatings using portable adhesion testers  Equipment not available

  10. PULL-OFF TEST Direction ection of force ce Top Doll lly Adhes esive Coating ing Substrate Adhes esive Bottom om Doll lly http://www.defelsko.com

  11. METHODS  Permeability  ASTM F1249 Standard test method for water vapor transmission rate through plastic film and sheeting using modulated infrared sensor  ASTM D3985 Standard test method for oxygen gas transmission rate through plastic film and sheeting using a coulometric sensor

  12. METHODS  Permeability  Equipment  Mocon Oxtran (oxygen)  Permatran (water vapor)

  13. CONCLUSIONS  Standard methods are useful for validated testing in research  Testing can be done more reliable and quicker with validated methods  Developed methods are used when the standard methods do not apply to the research conditions

  14. THANK YOU!!!

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