Ms. P. Vijaya Lakshmi Senior Research Fellow Department of Plant - - PowerPoint PPT Presentation

Presentation by

• Ms. P. Vijaya Lakshmi

Senior Research Fellow Department of Plant Physiology

• Dr. S. R. Voleti Dr. D. Subrahmanayam

Principal Scientist & Head Principal Scientist

Screening of Parental lines for Nitrogen Use Efficiency

 Around 78 Aromatic rice varieties were collected from Plant Breeding department, DRR, Hyderabad

Physiological Parameters

 Chlorophyll estimation by (SPAD  Leaf Thickness (Model 06-664-16, Fisher Scientifics, USA)  Leaf area ( Leaf area meter, LI-COR Environmental, USA)  Leaf Temperature ( Infra red thermometer, Made, ,model)  Leaf Rolling ( DRR method : in vivo)

Yield Parameters

 Panicle weight  Panicle Number  Filled grain weight  Unfilled grain weight  Total grain weight  Dry weight  Total dry weight &  Harvest index

Morphological Parameters

 Plant height  Tillers  Effective booting tillers

 Low Nitrogen Use efficiently rice varieties

• 5. Kola Joha 3
• 6. Ratnasundari

Genotypes Selected Based on overall physiological & yield traits

 High Nitrogen Use efficiently rice varieties

• 1. Basmathi 370
• 2. Ranbir Basmathi
• 3. Dehradun Basmati
• 4. Krishna Joha

N-0 field at vegetative stage N-100 field at vegetative stage

Breeding and Molecular work

 The selected genotypes were crossed reciprocally in the year of Kharif 2012 and collected the F1 seed.  The parental polymorphism was done by using SSR primers. Selected Polymorphic primers were used for F1 conformation.  In Rabi 2014, 4 cross were selected out of 15 crosses. The selected cross of f1 seed material were sown in field condition above said 4 population will be used for mapping.

Polymorphism studies between different selected parents

P1 F1 P2

 F2 populations of the selected crosses were planted in N-100 and N-0 plots in field, number of F2 plants in each cross in N-100 and N-0 are listed below. CROSS NAME No of F2's in N-100 No of F2's in N-0 (A1) Basmati 370 X BPT 5204 167 120 (A3) Basmati 370 X IR64 188 200 (A4) Dehradun basmati X BPT 5204 135 160 (A6) Dehradun basmati X IR64 168 112

F1 Conformation by using SSR primers

P2 P1 F1

 The leaf material was collected from selected genotypes at different N levels during different growth stages like vegetative and Reproductive.  The collected leaf was grinded with liquid nitrogen and stored at -20 º c. The Glutamine synthetase activity was measured in 120 genotypes at vegetative stage, remaining GOGAT and GDH activity is processing.  The quantity measurement of photorespiration was standardised and done in fifty field samples at vegetative stage and also standardised nitroprusside method for N estimation.

 Below Listed Parameters were Measured in High and Low Nue genotypes

 GS, NR  Chlorophyll Florescence  Photosynthetic Pigment  Photosynthesis  SLA , SLW, Thickness and SPAD

Biochemical Work

Mean of High and Low NUE genotypes at Different N levels

Agronomic efficiency Physiological efficiency

Nitrogen Use Efficiencies

 AE= Agronomic Efficiency (Kg/Kg)  PE= Physiological Efficiency  APE= Agro Physiological Efficiency  ARE=Apparent Recovery Efficiency  RE= Recovery Efficiency

Agro physiological efficiency Apparent recovery Efficiency Utilization Efficiency

Salient Findings

Publications:

 P. Vijayalakshmi • T. Vishnu Kiran • Y. Venkateswara Rao • B. Srikanth • I. Subhakara Rao •

• B. Sailaja • K. Surekha • P. Raghuveer Rao • D. Subrahmanyam • C. N. Neeraja • S. R. Voleti

“The Physiological approaches for increasing nitrogen use efficiency in rice crop” and was published in Indian journal of Plant Physiology. Ind J Plant Physiol. (July–September 2013) 18(3):208–222.  T. Vishnu Kiran, N. Sravan Raju, P. Senguttuvel, P. Vijayalakshmi, Y. Venkateswara Rao, K. Surekha, C.N. Neeraja, and S.R. Voleti. "Screening of Hybrids and parental lines for association

• f physiological traits to identify heat tolerant and nitrogen use efficient genotypes in rice

(Oryza sativa L)" was submitted for proceedings of 6th international hybrid rice symposium. (In press).  P. Vijayalakshmi • T. Vishnu Kiran • Y. Venkateswara Rao • B. Srikanth • I. Subhakara Rao •

• P. Raghuveer Rao • D. Subrahmanyam • C. N. Neeraja • S. R. Voleti “Screening of aromatic

rice for NUE and influence of Different Nitrogen levels on scavenging enzyme activity in rice (Oryza sativa L.), was submitted to Indian journal of Plant Physiology. (In Communicated).  A written a part of biochemical work in Bulletin entitled on Nitrogen Use Efficiency in irrigated rice for climate change – a case study.

Salient Findings

Abstracts and Poster Presentation:

 C.N. Neeraja, S.R. Voleti, K. Surekha, D. Subrahmanyam, I. Subhakararao, B. Srikanth, P. Vijayalakshmi, N. Sailaja, P. Revathi, B.C. Viraktamath (India). P0024 Identification of Genotypes, Genomic Regions And Genes for Nitrogen Use Efficiency in Rice. Presented at 7th International Rice Genetics Symposium. 5 - 8 November 2013 Dusit Thani Hotel, Manila, Philippines

Training programs:

 I was actively participated in one day work shop entitled on Nitrogen use efficiency in Rice at Directorate of Rice Research, Hyderabad. (23rd September).  I was attended training program entitled Awareness – cum-training programme on protection of plant varieties and farmers Rights Act 2001 and its Provisions at Directorate

• f Rice Research, Hyderabad (21st December 2013).

Future plan of work

 Leaf collection from F2 populations and DNA isolations.  Phenotypic observation will be recorded in population and parents, as mentioned below  Heading date (50% flowering),  Plant height at maturity, no of tillers, no of productive tillers,  SPAD of flag leaf,  Yield parameters,  Biomass, N content in grains.

Acknowledgement

My sincere thanks to Dr. B. C. Viraktamath, Project Director, DRR for

• verall supervision and also gratitude to

Dr. S. R. Voleti

for his great encouragement and valuable guidance. Acknowledge the NICRA (ICAR) Delhi, financial support for doing an innovative research work.