Modeling Wetlab Medical Engineering Human practices Male patient - - PowerPoint PPT Presentation

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Modeling Wetlab Medical Engineering Human practices Male patient - - PowerPoint PPT Presentation

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Modeling Wetlab Medical Engineering Human practices Male patient 11 years old Diagnosis ? GGBP Vyas N, Vyas M, Quiocho F (1988) Sugar and signal-transducer binding sites of the Escherichia coli galactose chemoreceptor protein. Science. 242,

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Wetlab Human practices Engineering Modeling Medical

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Male patient 11 years old

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Diagnosis ?

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Vyas N, Vyas M, Quiocho F (1988) Sugar and signal-transducer binding sites of the Escherichia coli galactose chemoreceptor protein. Science. 242, (4883) pp. 1290 - 1295

GGBP

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Baumgartner, et al. "Transmembrane signalling by a hybrid protein: communication from the domain of chemoreceptor Trg that recognizes sugar-binding proteins to the kinase/phosphatase domain of osmosensor EnvZ." J

  • Bactriol. (1994)

Trg two component system

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Feng X, Oropeza R, Walthers D, Kenney L (2003). OmpR Phosphorylation and Its Role in Signaling and

  • Pathogenesis. ASM News, 69, (8). pp. 390-395

EnvZ two component system

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Baumgartner, et al. "Transmembrane signalling by a hybrid protein: communication from the domain of chemoreceptor Trg that recognizes sugar-binding proteins to the kinase/phosphatase domain of osmosensor EnvZ." J

  • Bactriol. (1994)

TrgZ chimera

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Modeling

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Negative feedback system

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Bacterial Growth

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Glucose-insulin Dynamics

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Engineering

  • Great value.
  • Functionality.
  • Easy of use.
  • Great design.
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SAFETY OF THE USER

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Modular arrangment

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Contention Module

Polymer such as birth control subdermal implant

  • r biocompatible PET/PE
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Communication Module

Material: stainless steel- X15Cr13 and polymer of EPP-PEEK Ketron LSG

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Iris mechanism simulation

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External Module

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Finite Element Analysis

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Five bars with eccentric gear mechanism

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Mechanism simulation

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External module simulation

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Three E. coli strains were used: EnvZ knock-in, Rosettagami*

and SHuffle* ✓Strains’ growth curves characterization. ✓Measurement of the response to different glucose levels

  • f EnvZ knock-in strain with a reporter gene.

✓Assembly of a codon optimized TrgZ chimera for E. coli as well as a proinsulin lispro part with a TAT secretion signal. ✓Constitutive expression of proinsulin lispro in SHuffle and Rosettagami strains.

Results

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✓Strains’ growth curves characterization. ✓Measurement of the response to different glucose levels of EnvZ knock-in strain with a reporter gene. ✓Assembly of a codon optimized TrgZ chimera for E. coli as well as a proinsulin lispro part with a TAT secretion signal. ✓Constitutive expression of proinsulin lispro in SHuffle and Rosettagami strains.

Results

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✓Strains’ growth curves characterization. ✓Measurement of the response to different glucose levels of EnvZ knock-in strain with a reporter gene. ✓Assembly of a codon optimized TrgZ chimera for E. coli as well as a proinsulin lispro part with a TAT secretion signal. ✓Constitutive expression of proinsulin lispro in SHuffle and Rosettagami strains.

Reporter BBa_M30011

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Biobrick Design gBlock synthesis Gibson assembly Restriction analysis Transformation Western Blot

✓Strains’ growth curves characterization. ✓Measurement of the response to different glucose levels of EnvZ knock-in strain with a reporter gene. ✓Assembly of a codon optimized TrgZ chimera for E. coli as well as a proinsulin lispro part with a TAT secretion signal. ✓Constitutive expression of proinsulin lispro in SHuffle and Rosettagami strains.

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✓Strains’ growth curves characterization. ✓Measurement of the response to different glucose levels of EnvZ knock-in strain with a reporter gene. ✓Assembly of a codon optimized TrgZ chimera for E. coli as well as a proinsulin lispro part with a TAT secretion signal. ✓Constitutive expression of proinsulin lispro in SHuffle and Rosettagami strains.

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Collaboration with UNAH

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Collaboration with UNAH

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Collaboration with UNAH

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UNAH-UNAM

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Human Practices

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Talks about the model at RIIEME

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Wetlab Human practices Engineering Modeling Medical

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Credits

Team Members

Alejandro Guzmán - Storyteller Frida Reyes - Physician Lizbeth Bolaños - Researcher Alfredo López - Mathematical biologist Diego Galavíz - Engineer Xrysw Hernández - Accomplishments mentioning Alejandro Rodríguez Carlos Gil César Carreón

Advisors Pablo Padilla Marco Herrera Eduardo Rodríguez José Alonso Roberto Arreguín Acknowledgments Gloria Soberón Roberto Arreguín Javier Mancilla Joanna Hernández

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u[1] "Getting Syrian children back to school in Lebanon (11174110673)" by DFID - UK

Department for International Development - Getting Syrian children back to school in LebanonUploaded by russavia. Licensed under CC BY 2.0 via Wikimedia Commons - https://commons.wikimedia.org/wiki/File:Getting_Syrian_children_back_to_school_in_Leban

  • n_(11174110673).jpg#/media/File:Getting_Syrian_children_back_to_school_in_Lebanon_(11

174110673).jpg

  • [2] "Hospital room ubt" by Tomasz Sienicki [user: tsca, mail: tomasz.sienicki at gmail.com] - Own work. Licensed under Public Domain via Wikime

Commons - https://commons.wikimedia.org/wiki/File:Hospital_room_ubt.jpeg#/media/File:Hospital_room_ubt.jpeg [3]"Insulin". Licensed under CC BY-SA 3.0 via Wikimedia Commons - https://commons.wikimedia.org/wiki/File:Insulin.jpg#/media/File:Insulin.jpg [4]"Alpha-D-Glucopyranose" by NEUROtiker - Own work. Licensed under Public Domain via Wikimedia Commons - https://commons.wikimedia.org/wiki/File:Alpha-D-Glucopyranose.svg#/media/File:Alpha-D-Glucopyranose.svg