Listening to your Biosafety Cabinet Alarms, Response and Training - - PowerPoint PPT Presentation

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Listening to your Biosafety Cabinet Alarms, Response and Training - - PowerPoint PPT Presentation

Listening to your Biosafety Cabinet Alarms, Response and Training David S. Phillips, DM The world leader in serving science Early BSC Controls and Display BLOWER CABINET OUTLETS CIRCUIT CIRCUIT BREAKER BREAKER LIGHTS 2 Pressure


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The world leader in serving science

David S. Phillips, DM

Listening to your Biosafety Cabinet – Alarms, Response and Training

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Early BSC Controls and Display

BLOWER LIGHTS

CABINET

CIRCUIT BREAKER

OUTLETS

CIRCUIT BREAKER

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Pressure Gauges HEPA Filter Blower

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Pressure Gauges – A question 0.37” 0.37” 0.37” 0.40” 0.40” 0.50” 0.55” 0.65” 0.66”

In a survey of the same model cabinet at a facility

9 readings 0.37” to 0.66” w.g.

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Pressure Gauges – Manufacturer Guidance

  • “When the reading increases by 50% from original measurement, cabinet

airflow should be checked with a thermoanemometer. Replace the filters if proper airflow cannot be obtained.” ThermoForma 1284

  • “The initial pressure reading will be approximately 0.5" w.g. + 0.1" w.g.

depending on altitude from sea level. At each 0.1" w.g. increment increase, the cabinet airflow should be checked by a qualified technician, unless certified on a yearly (or sooner) basis.” NuAire NU425-400 Series 30

  • “. . . check the reading on the analog pressure gauge; the displayed value

should remain consistent with the recorded value in the most recent certification report. A significant change in pressure should be cause for

  • investigation. This device is not intended to be used for air flow set-point

verification.” Baker SG404

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Example of current BSC interface Blower ON Blower pending Blower Alarm Light ON UV Light ON Outlets ON Any alarm condition Alarms

New Firmware Loaded Internal Board Failure Power on Reset Airflow Pressure Alarm Blower RPM Failure Window High Window Low Replace UV Light

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Example of current BSC interface

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  • Sliding Window Alarm
  • Audible and Visual - 1 inch (25 mm) above 1 inch (25 mm) below

NSF/ANSI 49 Alarm and Interlock Requirements

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  • External Exhaust alarms

NSF/ANSI 49 Alarm and Interlock Requirements

  • For B1 and B2 - Audible and Visual

alarm and cabinet interlock shut- down within 15 seconds of exhaust volume loss exceeding 20%.

  • For Canopied A1 and A2 - Audible

and Visual alarm within 15 seconds

  • f a loss of capture of room air at the

canopy air intake(s). Cabinet continues to operate.

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  • External Exhaust alarms

NSF/ANSI 49 Alarm and Interlock Requirements

  • For B1 and B2 - Audible and Visual

alarm and cabinet interlock shut- down within 15 seconds of exhaust volume loss exceeding 20%.

  • For Canopied A1 and A2 - Audible

and Visual alarm within 15 seconds

  • f a loss of capture of room air at the

canopy air intake(s). Cabinet continues to operate.

Canopy Gap

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  • External Exhaust alarms

NSF/ANSI 49 Alarm and Interlock Requirements

  • For B1 and B2 - Audible and Visual

alarm and cabinet interlock shut- down within 15 seconds of exhaust volume loss exceeding 20%.

  • For Canopied A1 and A2 - Audible

and Visual alarm within 15 seconds

  • f a loss of capture of room air at the

canopy air intake(s). Cabinet continues to operate.

Canopy Gap

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Canopy Alarms Canopy Gap

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BSC Alarm Overview

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BSC Alarm Overview

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BSC Alarm Procedures Do not make rapid movements Cover exposed materials Secure work area Close window Wash hands and clean up

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BSC Alarm Procedures If not working with volatile toxic chemicals, you can continue

  • working. Probably you will want to

finish up because of the noise.

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BSC Alarm Procedures If not working with hazardous materials, you should finish up within 5 minutes. Do not make rapid movements Cover exposed materials Secure work area Close window Wash hands and clean up If working with hazardous materials,

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Suggestions Window Position Blower (normal) Blower (Stand-by) External Exhaust (for fumes)

  • Quick Guide and Table of Responses
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Class II BSCs are great!

Dave Phillips david.phillips@thermofisher.com

“Why don’t I get a respirator?”