Forensic Toxicology & Drug Katherine Sato Office of the Ohio - - PowerPoint PPT Presentation

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Forensic Toxicology & Drug Katherine Sato Office of the Ohio - - PowerPoint PPT Presentation

Forensic Toxicology & Drug Katherine Sato Office of the Ohio Public Chemistry for Defense Attorneys Defender Introduction Forensic Toxicology Outline of Alcohol/OVI Testing Presentation Drug Field Testing Drug Chemistry for the


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Forensic Toxicology & Drug Chemistry for Defense Attorneys

Katherine Sato Office of the Ohio Public Defender

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Outline of Presentation

Introduction Forensic Toxicology Alcohol/OVI Testing Drug Field Testing Drug Chemistry for the Defense

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Various Fields May Apply Forensic Chemistry Forensic Toxicology Pharmacology Pharmacogenetics and Pharmacokinetics

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Forensic Chemistry

The application of analytical techniques in a legal setting for the purpose of identification of unknown chemical substances

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Forensic Toxicology

Employs the analytical techniques of chemistry for the detection of drugs, toxins, and/or pharmaceuticals in biological samples

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Pharmacology

Both the overt effects of biologically active chemicals and the mechanism by which these chemicals interact with each other and biological targets

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Pharmacokinetics

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Pharmacogenetics

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Expert Testimony

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Who Can Testify?

Right to confrontation May need to circumscribe an expert’s testimony Cross-discipline fields → may need multiple experts

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Melendez-Diaz v. Massachusetts (2009)

SCOTUS: defendants have a right to confront laboratory analysts Held: 1. Analysts’ certificates of analysis are testimonial statements; 2. Analysts are not removed from coverage

  • f Confrontation Clause

3. Defendant’s ability to subpoena analyst did not obviate State’s obligation to produce analyst for cross-examination

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Melendez-Diaz v. Massachusetts (2009)

“Nor is it evident that what respondent calls ‘neutral scientific testing’ is as neutral or as reliable as respondent suggests. Forensic evidence is not uniquely immune from the risk of manipulation. According to a recent study conducted under the auspices of the National Academy of Sciences, ‘[t]he majority of [laboratories producing forensic evidence] are administered by law enforcement agencies, such as police departments, where the laboratory administrator reports to the head of the agency.’ * * * And ‘[b]ecause forensic scientists often are driven in their work by a need to answer a particular question related to the issues of a particular case, they sometimes face pressure to sacrifice appropriate methodology for the sake of expediency.’ A forensic analyst responding to a request from a law enforcement official may feel pressure—or have an incentive—to alter the evidence in a manner favorable to the prosecution.”

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R.C. 2925.51

In any criminal prosecution, a laboratory report from BCI (or other accredited lab) stating:

  • 1. That the substance has been weighed and

analyzed;

  • 2. The findings as to the content, weight, and

identity of the substance;

  • 3. That it contains any amount of a controlled

substance; and

  • 4. The number and description of unit dosages

Is “prima facie evidence” of the content, identity, and weight of the substance.

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State v. Pasqualone (OSC 2009)

When the state has complied with its

  • bligations under R.C. 2925.51, a

defendant’s failure to use the procedures of R.C. 2925.51(C) to demand that a laboratory analyst testify constitutes a waiver of the

  • pportunity to cross-examine the

analyst at trial and allows the analyst’s report to be admitted as prima facie evidence of the test results.

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Bullcoming v. New Mexico (2011)

“We hold that surrogate testimony * * * does not meet the constitutional requirement. The accused’s right is to be confronted with the analyst who made the certification, unless that analyst is unavailable at trial, and the accused had an opportunity, pretrial, to cross-examine that particular scientist.” “SLD analysts use gas chromatograph machines to determine BAC levels. Operation of the machines requires specialized knowledge and training. Several steps are involved in the gas chromatograph process, and human error can occur at each step.”

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“Drug Recognition Experts”

  • Made up field/ mostly

nonscientists

  • One week-long training and they

claim to identify whether a person was on PCP vs. heroin

  • Challenge on scientific basis for

their observations

  • Do not have knowledge of how

drugs affect the human body

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Forensic Toxicology

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Metabolism

  • The whole range of biochemical processes

that occur within a living organism

  • Consists of anabolism (the buildup of

substances) and catabolism (the breakdown

  • f substances)
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Metabolism

  • Enzymes convert drugs into metabolites
  • Metabolites may be therapeutic, harmful, or

inactive

  • Cannot use metabolites as indicator of

impairment (not psychoactive)

  • May need expert in pharmacokinetics (to

determine how quickly individual metabolizes specific drug) if when the person took the drug is important

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Half-Life

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Metabolism of Cocaine

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Blood vs. Urine Detection

Blood

Can determine impairment Varies less by individuals More invasive

Urine

Cannot determine impairment Individual variation Less invasive, more common

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Post-Collection Changes

  • If a toxin is in the urine, it will stay there if

properly stored

  • Not going to continue breaking down
  • Some exceptions (e.g., if someone has a

lot of sugar and yeast in the urine, could change to alcohol after it leaves the body while stored)

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AG’s Guidelines

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Hair Testing

  • As someone is exposed to drugs, the

actively growing hair will sequester some of the drug

  • Once drugs deposited in hair shaft, remains

part of hair indefinitely

  • Drugs are deposited in the root and then

continue down the hair as it grows

  • Normally collect about 3 cm
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Alcohol/OVI Testing

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Types of OVI Field Tests

Standardized field sobriety tests Intoxilyzers

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Standardized Field Sobriety Tests

Three tests used in combination to determine intoxication: 1. Horizontal Gaze Nystagmus (HGN) – involuntary eye movement 2. Walk-and-Turn (WAT) – divided attention activity 3. One-Legged Stand – divided attention activity Should only be used for alcohol, not proper for marijuana or any other drugs

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Reliability of Standardized Field Sobriety Tests

National Highway Traffic Safety Administration (NHTSA) found that, in determining whether a subject’s BAC was .10 or above:

  • The HGN by itself was 77% accurate
  • The Walk-and-Turn by itself was 68%

accurate

  • The One-Leg Stand by itself was 65%

accurate

  • The HGN and WAT in combination

were 80% accurate One study found 24% false positive results

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Intoxilyzers

  • Device that measures ethanol expelled from

the lungs

  • Used alone or with SFSTs
  • Blood alcohol concentration (BAC) is then

estimated from breath alcohol concentration (BrAC) using a generalized ratio of BAC:BrAC = 2100:1

  • Can vary by individuals
  • No evidence this specific equation applies

to your client

  • Range in calculation could be the

difference between BAC of .079 and .08

  • Requires 15- to 20-minute waiting period
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Intoxilyzers: Potential Errors

One breath sample Calibration Temperature Non-selective Sample destroyed Assumes partition ratio of 2100:1

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Discovery Request for Intoxilyzers

Calibration information All solutions used to calibrate the machine Margin of error of all solutions used to calibrate the machine Maintenance of the instrument

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R.C. 4511.19(D)(1)(b)

  • “In any criminal prosecution or juvenile court proceeding for

[OVI], the court may admit evidence on the concentration of alcohol * * * in the defendant’s * * * breath * * * at the time

  • f the alleged violation as shown by chemical analysis of the

substance withdrawn within three hours of the time of the alleged violation.”

  • “The bodily substance withdrawn under division (D)(1)(b) of

this section shall be analyzed in accordance with methods approved by the director of health * * *”

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State v. Vega (OSC 1984)

Because the legislature provided for the admissibility of intoxilyzer tests if analyzed in accordance with methods approved by the director of ODH, an accused may not present expert testimony attacking the general scientific reliability of approved test instruments.

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Cincinnati v. Ilg (OSC 2014)

The approval of a breath-analyzer machine by the director of the Ohio Department of Health does not preclude an accused from challenging the accuracy, competence, admissibility, relevance, authenticity, or credibility of a specific test result or whether the specific machine used to test the accused operated properly at the time of the test.

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Retrograde Extrapolation

Can someone reliably estimate the amount of alcohol ingested from a single measurement of a person’s blood alcohol concentration (BAC)?

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Blood-Alcohol Curve

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Widmark Formula

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People v. Floyd (IL 2014)

“A retrograde extrapolation calculation based on a single breath test, and when many of the factors necessary to determine whether the defendant was in the elimination phase are unknown, is insufficient to provide a reliable calculation and invites the jury to determine guilt on an improper basis. Based on the specific circumstances presented in this case, we believe that the prejudicial effect of the retrograde extrapolation calculation substantially outweighed its probative value and that the trial court abused its discretion in admitting it.”

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State v. Armstrong (NV 2011)

The following factors are relevant to achieving a sufficiently reliable retrograde extrapolation calculation: (1) gender, (2) weight, (3) age, (4) height, (5) mental state, (6) the type and amount of food in the stomach, (7) type and amount of alcohol consumed, (8) when the last alcoholic drink was consumed, (9) drinking pattern at the relevant time, (10) elapsed time between the first and last drink consumed, (11) time elapsed between the last drink consumed and the blood draw, (12) the number of samples taken, (13) the length of time between the

  • ffense and the blood draws, (14) the average alcohol

absorption rate, and (15) the average elimination rate.

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Drug Field Tests

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Video

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Issues?

Reliability of field tests Differential treatment Plea bargain process

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Drug Color Test Kits

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Drug Color Test Kits in the News

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Best Practices in Positive Drug Color Field Test Cases

  • Give notice to preserve evidence and subsequently move for

confirmatory testing

  • Assist clients to mitigate the consequences of jail time while

they wait

  • Consult with client on the lasting consequences of a felony

conviction (compared to the immediate reward of pleading and getting out of jail)

  • Keep track of all cases where confirmatory tests in jurisdiction

show no controlled substances present

  • FOIA requests or subpoenas to get data on what is known

about false positive rates

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Drug Chemistry for the Defense

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Drug Chemistry for the Defense

Types of Laboratory Testing Potential Errors in Testing SWGDRUG Recommendations Accreditation Process Documents to Request

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Types of Laboratory Testing

Gas Chromatography/ Mass Spectrometry (GC/MS) Liquid Chromatography/ Mass Spectrometry (LS/MS) Thin Layer Chromatography (TLC) Color Tests

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Gas Chromatography/ Mass Spectrometry (GC/MS)

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Liquid Chromatography/ Mass Spectrometry (LS/MS)

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Thin Layer Chromatography (TLC)

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NIST Mass Spectral Library

  • One of the world’s largest and most

widely used database of organic compounds

  • Provides mass of more the 265,000

compounds

  • “When you line up the fragments in
  • rder of their mass-to-charge ratio, you

get the molecule’s distinctive ‘mass spectra,’ which looks like a barcode and functions like a fingerprint.”

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NIST Mass Spectral Library

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Potential Errors in Laboratory Testing of Controlled Substances

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2009 NAS Report

“Some laboratories might mention the tests that were conducted, but in most cases the spectra, chromatograms, and other evidence of the analysis and the chemist’s notes are not submitted. Likewise, possible sources of error and statistical data are not commonly

  • included. From a scientific perspective, this style of

reporting is often inadequate, because it may not provide enough detail to enable a peer or other courtroom participant to understand and, if needed, question the sampling scheme, process(es) of analysis, or interpretation.” (135)

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PCAST Report

“One analysis estimated that at least fifty major laboratories reported fraud by analysts, evidence destruction, failed proficiency tests, misrepresenting findings in testimony, or tampering with drugs between 2005 and 2011. Twenty-eight of these labs were nationally accredited.” (33, fn60)

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Massachusetts State Drug Lab Scandal

  • http://www.nbcnews.com/nightly-news/video/20-000-convictions-

dropped-after-massive-drug-lab-scandal-923957827606

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Potential Errors in Laboratory Chemical Analysis

  • Sample contamination
  • Erroneous calculations
  • Failure to calibrate instruments
  • Lack of proper education and training

Incompetence/Negligence

  • “Drylabbing”
  • Destruction of records to cover-up negligence

Fabrication/Deception

  • Receiving extraneous information about samples →
  • bserver bias
  • Pressure to produce findings favorable to law

enforcement Bias

  • Failure to state the testing performed and

procedures used Incomplete/Misleading Reports

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Carryover

  • If the concentration of the analyte is too great, it will

carry over from previous samples

  • Cocaine gives a very large response with minimal

amount of sample

  • NSAIDS (ibuprofen, naproxen, etc.) and THC carryover

into several samples

  • Need to run these tests last
  • Run several blanks after them
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SWGDRUG Recommendations

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Specific Provisions of SWGDRUG

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Accreditation Process

  • Inspectors come into lab, spread out

into different sections, and take notes (basically an audit)

  • The lab is on notice of the inspection
  • Inspectors ask perm

rmis ission from the lab technicians to give them files

  • Head of inspection team writes report

using individual inspectors’ notes

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Accreditation Requirements

  • Accrediting agencies not looking at science; rather, looking at

whether written procedures in place, whether there are quality control procedures

  • Any new accreditation must be based on ISO 17025

(international organization)

  • There are some legacy accreditations prior to ISO standard

(suspect)

  • ANAB National Accreditation Board
  • ASCLD/LAB American Society of Crime Laboratory Directors/

Laboratory Accreditation Board

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Documents to Request from Accredited Lab

1. Preliminary Assessment Report

  • What inspectors found when they went in the lab
  • What rules or regulations were violated (e.g., failure to fully explain

procedures so others can replicate process, failure to record key information, failure to perform tests, failure to follow lab manuals and procedures)

  • Request to correct violations

2. Corrective Action Request (CAR)

  • Lab’s response to preliminary assessment report
  • Admission to violating specific rules or regulations (usually in

memo form signed and submitted by section chief)

  • Explains how the lab will make changes

3. Final Assessment Report

  • Acknowledge changes to known violations
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Documents to Request from Accredited Lab

4. Laboratory Information Management System (LIMS) Printout

  • Computerized system to track cases
  • Computer printout will reveal whether anyone changed the

conclusion from the technician assigned to the case

  • Computer printout will also reveal whether evidence was

misplaced for a while 5. Quality Manual

  • Any laboratory accredited by certain organizations must have a

quality manual (if not, in violation of accreditation)

  • Ask for quality manual to determine what policies, procedures

the lab has prescribed for themselves

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Documents to Request from Accredited Lab

6. Nonconformity Reports

  • A document stating when two technicians/analysts in a lab

disagree (e.g., technician A finds a match, technician B finds it inconclusive, supervisor rules it a match)

  • Discoverable under Brady, but State usually doesn’t understand

meaning of the report

  • Can request all nonconformity reports for a specific technician
  • ver the past several years
  • Must be made even if final report never issued
  • Would also be referenced in LIMS

7. Root Cause Analysis (RCA)

  • Whenever a nonconformity occurs, lab must look into it further

to make a recommendation as to how to resolve the problem in the future

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Documents for Specific Instrument/Analyst

  • Standard Operating Procedures for specific instrument
  • Instruction manual for instrument
  • Instrument calibration data
  • Quality control data
  • Precision studies of the procedure and instrument
  • Interference studies of the procedure and instrument
  • Validation studies of the procedure and instrument
  • Results of all samples analyzed with this instrument and procedure on the date of testing
  • Training of all lab analysts on this procedure and instrument
  • Inspection/maintenance reports
  • Margin of error of this analysis with supporting documentation/studies
  • Any false positives
  • Discrepancy reports
  • Personnel records of individuals that performed testing
  • Proficiency testing of analyst
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Questions? Comments?

Katherine Sato Assistant State Public Defender, Juvenile Department katherine.sato@opd.ohio.gov