5/3/16 May 2, 2016 Channel Ecology Assignment for Weds, May 4 (Mon - PDF document

5/3/16 May 2, 2016 Channel Ecology Assignment for Weds, May 4 (Mon lab)/11 (Tues Lab) Pen your poison: http://www.amnh.org/exhibitions/current-exhibitions/the- power-of-poison Power of Poison: an enchanted book- Interactive botanical book Coming Due Dates: Extra credit helping a freshman physiology lab Monday May 2- Snail: Diversity of neuronal (BioNB 1445) excitability lab Monday, May 2- 7:30-8:30PM Friday, May 6, Snail: Ionic basis of excitability lab Tuesday, May 3- 9:05-10:05AM Weds, May 4, Monday lab-Pen your Poison people and 7:30-8:30PM Thursday, May 5, Fly results Monday, May 9- Fly report Weds, May 11, Tuesday lab- Pen your Poison Problems : Problems : Washout of cell metabolites Sometimes need clean membranes Activation of histamine release from mast cells Subconductance states- ACh receptors) Technology can determine results and interpretation- Anesthetic example Subconductance states Perforated patch recordings maintain intracellular integrity Chapman et al 1997 1

5/3/16 Examples of calcium APs Channel Ecology Complex Activity of Neurons Driven by Tailored Cha nnel Ecosystems Na AP vs. Heart AP Cardiac AP currents 2

5/3/16 Real neurons have many types of currents Common Inward and Outward Currents I K(V) I A Block sodium and potassium currents I K(Ca) Current I Ca I Na Voltage 4 I A 3 2 I M (nA) I K(V) I Ca 1 I K(Ca) 0 I Na -1 -2 -6 0 -4 0 -2 0 0 E M (mV) Interesting firing properties of Activation of different currents produces varied neuron activity neurons Snail bursterneurons Voltage 20 mV Current 10 s Mouse spinal cord burster neurons 3

5/3/16 Sleep Wake Thalamic relay neurons Sleep Wake B Switch from bursting to tonic spiking controlled by leak and I h voltage range Neuromodulation induces plasticity in neural networks Rhythmic Netw ork Flexibility: Period Network Members Firing Phases Firing Intensity Kloppenburg, Levini, and H arris-Wa rrick, 19 9 9 4

5/3/16 Currents can be modified by neuromodulators to change firing properties Currents modified by neuromodulators in the pyloric network Ionic Targets of Dopamine in Pyloric N etwork PY rebound firing is timed by its intrinsic properties, also modulated by DA I A , I h , I Ca 5