5/3/16 May 2, 2016 Channel Ecology Assignment for Weds, May 4 (Mon - - PDF document

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May 2, 2016

Coming Due Dates: Monday May 2- Snail: Diversity of neuronal excitability lab Friday, May 6, Snail: Ionic basis of excitability lab Weds, May 4, Monday lab-Pen your Poison people Thursday, May 5, Fly results Monday, May 9- Fly report Weds, May 11, Tuesday lab- Pen your Poison Extra credit helping a freshman physiology lab (BioNB 1445) Monday, May 2- 7:30-8:30PM Tuesday, May 3- 9:05-10:05AM and 7:30-8:30PM

Channel Ecology

Assignment for Weds, May 4 (Mon lab)/11 (Tues Lab) Pen your poison: http://www.amnh.org/exhibitions/current-exhibitions/the- power-of-poison Power of Poison: an enchanted book- Interactive botanical book Sometimes need clean membranes Subconductance states- ACh receptors) Technology can determine results and interpretation- Anesthetic example Problems:

Chapman et al 1997

Subconductance states Perforated patch recordings maintain intracellular integrity

Problems: Washout of cell metabolites

Activation of histamine release from mast cells

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Channel Ecology

Complex Activity of Neurons Driven by Tailored Cha nnel Ecosystems

Examples of calcium APs Na AP vs. Heart AP Cardiac AP currents

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INa IA IK(V)

4 3 2 1

• 1
• 2
• 6 0
• 4 0
• 2 0

EM(mV) IM(nA)

IK(Ca) IA IK(V) IK(Ca) INa Voltage Current Block sodium and potassium currents Real neurons have many types of currents ICa ICa Common Inward and Outward Currents

Voltage Current

Activation of different currents produces varied neuron activity Interesting firing properties of neurons

10 s 20 mV

Snail bursterneurons Mouse spinal cord burster neurons

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Thalamic relay neurons Sleep Wake Sleep Wake

B Switch from bursting to tonic spiking controlled by leak and Ih voltage range Rhythmic Netw

• rk Flexibility:

Period Network Members Firing Phases Firing Intensity

Kloppenburg, Levini, and H arris-Wa rrick, 19 9 9

Neuromodulation induces plasticity in neural networks

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PY rebound firing is timed by its intrinsic properties, also modulated by DA IA , Ih , ICa

Currents can be modified by neuromodulators to change firing properties Ionic Targets of Dopamine in Pyloric N etwork

Currents modified by neuromodulators in the pyloric network